We narrowed to 9,006 results for: Pol;

1. RPO41 deltaN296

   Plasmid

   #205075

   Purpose

   RPO41 protein expression

   Depositor

   Smita Patel

   Article

   Paratkar et al J Biol Chem. 2011 May 6;286(18):16109-20

   Insert

   RPO41 (RPO41 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Lacks N Terminal 296 amino acids

   Promoter

   Available since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-Blast-Dest-LifeAct-mRuby

   Plasmid

   #194907

   Purpose

   To generate LifeAct-mRuby expressing stable cell lines with piggybac transposase co-expression and blastocidin selections.

   Depositor

   Clare Buckley

   Article

   Liang et al EMBO J. 2022 Aug 22:e111021. doi: 10.152

   Insert

   LifeAct
   

   Use

   Mouse Targeting

   Tags

   mRuby

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDT2

   Plasmid

   #199119

   Purpose

   circular transcription template

   Depositor

   Jeff Gelles

   Article

   Inlow et al Proc Natl Acad Sci U S A. 2023 Jul 11;12

   Insert

   lambda PR' - repeat cassette - E. coli rpoB - lambda TR'
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   mutated 5′-CTGGAGTGCG-3′ to 5′-CTGGAGACCG-3′ to …

   Promoter

   Available since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBetaActin-dCer3-C2-FRB

   Plasmid

   #191327

   Purpose

   Expresses the fluorescently labeled membrane anchor C2 fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRB

   Depositor

   Frank Bradke

   Article

   Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

   Insert

   FRB-C2-dCer3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMpGWB302-er-mCherry

   Plasmid

   #186287

   Purpose

   Binary vector for the expression of er-mCherry (SP-mCherry-HDEL) in plants (for Agrobacterium-mediated genetic transformation and visualization of endoplasmic reticulum)

   Depositor

   Yutaka Kodama

   Article

   Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

   Insert

   er-mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCI-neo-μ3A-(HA)3

   Plasmid

   #198179

   Purpose

   Expression of HA-tagged AP-3 μ3A in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Mattera et al PLoS Pathog. 2014 May 15;10(5):e1004107.

   Insert

   AP-3 μ3A
   

   Use

   Tags

   triple HA tag

   Expression

   Mammalian

   Mutation

   silent substitution in codon 225

   Promoter

   CMV

   Available since

   April 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBetaActin-FRB-dCer3-CAAX

   Plasmid

   #191326

   Purpose

   Expresses the fluorescently labeled membrane anchor CAAX fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRB

   Depositor

   Frank Bradke

   Article

   Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

   Insert

   FRB-dCer-CAAX
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBetaActin-TUBB2a-mEos3.2

   Plasmid

   #191320

   Purpose

   Expresses fuorescently labeled beta tubulin to allow photoconversion of microtubule patches from green to red fluorescent with UV light

   Depositor

   Frank Bradke

   Article

   Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

   Insert

   TUBB2a-mEos3.2 (Tubb2a Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBetaActin-TUBB2a-Dronpa

   Plasmid

   #191321

   Purpose

   Expresses fuorescently labeled beta tubulin to allow photoactivation of microtubule patches with very little UV light

   Depositor

   Frank Bradke

   Article

   Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

   Insert

   TUBB2a-Dronpa (Tubb2a Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMpGE_En03-sgRNA-Target1 (MpRTN1)

    Plasmid

    #186291

    Purpose

    Gateway entry vector for sgRNA (target 1: MpRTN1). Transient expression of sgRNA (Target 1: MpRTN1) for MpRTN1 in plant cells

    Depositor

    Yutaka Kodama

    Article

    Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

    Insert

    sgRNA-Target1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYTK001_E2At(2nd)

    Plasmid

    #183085

    Purpose

    2A peptide E2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in tri-cistronic and third 2A peptide in quad-cistronic construct assembly

    Depositor

    Zhen Wang

    Article

    Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

    Insert

    E2A
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pYTK001_O2At(2nd)

    Plasmid

    #183086

    Purpose

    2A peptide O2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in tri-cistronic and third 2A peptide in quad-cistronic construct assembly

    Depositor

    Zhen Wang

    Article

    Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

    Insert

    O2A
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pYTK001_P2At(2nd)

    Plasmid

    #183087

    Purpose

    2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in tri-cistronic and third 2A peptide in quad-cistronic construct assembly

    Depositor

    Zhen Wang

    Article

    Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

    Insert

    P2A
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMpGE010-sgRNA-Target1 (MpRTN1)

    Plasmid

    #186295

    Purpose

    Binary vector for CRISPR/Cas9 (target 1: MpRTN1) in plants (for Agrobacterium-mediated genetic transformation)

    Depositor

    Yutaka Kodama

    Article

    Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

    Insert

    sgRNA-Target1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET-11-LECT2-V40

    Plasmid

    #183031

    Purpose

    Expression of LECT2 V40 variant protein from T7 promoter

    Depositor

    Gareth Morgan

    Article

    Belonogov et al bioRxiv 2022.03.01.482540

    Insert

    LECT2 (LECT2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    V40 variant

    Promoter

    T7

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pZS181

    Plasmid

    #177637

    Purpose

    In vitro transcription of Patiria miniata Dishevelled

    Depositor

    Iain Cheeseman

    Article

    Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

    Insert

    Dishevelled
    

    Use

    In vitro transcription

    Tags

    HaloTag

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pZS212

    Plasmid

    #177639

    Purpose

    In vitro transcription of Patiria miniata Lamp1

    Depositor

    Iain Cheeseman

    Article

    Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

    Insert

    Lamp1
    

    Use

    In vitro transcription

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pZS217

    Plasmid

    #177640

    Purpose

    In vitro transcription of Patiria miniata Dishevelled

    Depositor

    Iain Cheeseman

    Article

    Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

    Insert

    Dishevelled
    

    Use

    In vitro transcription

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pZS221

    Plasmid

    #177643

    Purpose

    In vitro transcription of Patiria miniata Dishevelled

    Depositor

    Iain Cheeseman

    Article

    Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

    Insert

    Dishevelled
    

    Use

    In vitro transcription

    Tags

    GFP

    Expression

    Mutation

    K57A, K58A

    Promoter

    Available since

    Dec. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pZS222

    Plasmid

    #177644

    Purpose

    In vitro transcription of Patiria miniata Lamp1

    Depositor

    Iain Cheeseman

    Article

    Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

    Insert

    Lamp1
    

    Use

    In vitro transcription

    Tags

    3xGFP

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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