We narrowed to 6,371 results for: siae
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Plasmid#121405PurposeExpressing Atg17DepositorInsertCUP1p-ATG17 ORF-CYC1 terminator
ExpressionYeastMutationLeucine 105 to Aspartic acidPromoterCUP1 promoterAvailable SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCu-Atg17(416) (F317D)
Plasmid#121406PurposeExpressing Atg17DepositorInsertCUP1p-ATG17 ORF-CYC1 terminator
ExpressionYeastMutationPhenylalanine 317 to Aspartic acidPromoterCUP1 promoterAvailable SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCu-Atg17(416) (I325D)
Plasmid#121407PurposeExpressing Atg17DepositorInsertCUP1p-ATG17 ORF-CYC1 terminator
ExpressionYeastMutationIsoleucine 325 to Aspartic acidPromoterCUP1 promoterAvailable SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRPL28exon1-(Nhe/Mfe) SpHIS5 (pNTI619)
Plasmid#115432PurposeVector for expressing RPL28 with mutagenized exon2DepositorInsertsExpressionYeastMutationexon 2 deleted, with MfeI / NheI cloning sitePromoterAshbya gospii TEF1 and endogenousAvailable SinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNIA-CEN-FLAG-LINXa4-Bre1
Plasmid#73617PurposeControl of the E3 ligase Bre1 with LINXa in yeast.DepositorInsertLINXa4-Bre1dNLS
UseSynthetic BiologyTagsFLAGExpressionYeastMutationBre1 point mutations K8A, K9A and K11APromoterADH1Available SinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2 - human TUBB1 Cterminal tail
Plasmid#60400PurposeExpression of chimeric yeast beta tubulin (TUB2) - human beta6 tubulin Cterminal tail (TUBB1) using a GAL promoterDepositorInsertTUB2
ExpressionYeastMutationTUB2 amino acids 429 - end, replaced with human T…PromoterGALAvailable SinceMay 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2 - human TUBB3 Cterminal tail
Plasmid#60397PurposeExpression of chimeric yeast beta tubulin (TUB2) - human beta3 tubulin Cterminal tail (TUBB3) using a GAL promoterDepositorInsertTUB2
ExpressionYeastMutationC127S; TUB2 amino acids 429 - end, replaced with …PromoterGALAvailable SinceMay 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRevLCav2FeS
Plasmid#60808Purposeto disrupt iron sulfur cluster in yeast Rev3DepositorInsertREV3 (REV3 Budding Yeast)
ExpressionYeastMutationcontains 647 C-terminal amino acids and 335 bp of…Available SinceMarch 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2 - human TUBB7 Cterminal tail
Plasmid#60399PurposeExpression of chimeric yeast beta tubulin (TUB2) - human beta7 tubulin Cterminal tail (TUBB7) using a GAL promoterDepositorInsertTUB2
ExpressionYeastMutationTUB2 amino acids 429 - end, replaced with human T…PromoterGALAvailable SinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2 - human TUBB8 Cterminal tail
Plasmid#60401PurposeExpression of chimeric yeast beta tubulin (TUB2) - human beta8 tubulin Cterminal tail (TUBB8) using a GAL promoterDepositorInsertTUB2
ExpressionYeastMutationTUB2 amino acids 429 - end, replaced with human T…PromoterGALAvailable SinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2 - human TUBB4a Cterminal tail
Plasmid#60402PurposeExpression of chimeric yeast beta tubulin (TUB2) - human beta4a tubulin Cterminal tail (TUBB4a) using a GAL promoterDepositorInsertTUB2
ExpressionYeastMutationTUB2 amino acids 429 - end, replaced with human T…PromoterGALAvailable SinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAV10.K3.loxP
Plasmid#63202PurposepCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI).DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailabilityAcademic Institutions and Nonprofits only -
pAV10.K6.loxP
Plasmid#63203PurposepCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailabilityAcademic Institutions and Nonprofits only -
pAV10.K5.loxP
Plasmid#63204PurposepCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailabilityAcademic Institutions and Nonprofits only -
pAV10.HO3.loxP
Plasmid#63205PurposepCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI).DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailabilityAcademic Institutions and Nonprofits only -
pAV10.HO6
Plasmid#63211PurposepCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into HO locus (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailabilityAcademic Institutions and Nonprofits only -
pDSM-de-Ppgk1-PYC-Ttpi1
Plasmid#127735PurposeYeast pathway position 5. PYC transcription unit with the PGK1 promoter and TPI1 terminator.DepositorInsertpyruvate carboxylase (PYC1 Budding Yeast, Synthetic)
UseSynthetic BiologyExpressionYeastPromoterPpgk1AvailabilityAcademic Institutions and Nonprofits only -
pDSM-de-Ppxr1-PYC-Tnat1
Plasmid#127736PurposeYeast pathway position 5. PYC transcription unit with the PXR1 promoter and NAT1 terminator.DepositorInsertpyruvate carboxylase (PYC1 Budding Yeast, Synthetic)
UseSynthetic BiologyExpressionYeastPromoterPpxr1AvailabilityAcademic Institutions and Nonprofits only -
Yiplac211 proGAL1-TsCC(A)-RGG-GFP-RGG-RGG
Plasmid#176395PurposeExpresses GFP tagged triple RGG scaffold with N-terminal TsCC(A) coiled coil. Expression induced in media containing galactose.DepositorInsertGAL1 promoter, TsCC(A)-RGG-GFP-RGG-RGG
ExpressionYeastPromoterGAL1Available SinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
HDAC4 Flag
Plasmid#13821PurposeMammalian expression of human histone deacetylase 4 with flag tagDepositorAvailable SinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDisCoTune
Plasmid#173713PurposePromotes expression of disulfide-rich protein in E. coli T7 expression system. The plasmid encodes expression of the folding factors Erv1p and hPDI controlled by the Ptac promoter.DepositorInsertsExpressionBacterialAvailable SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGTM_YR375_SUMO_Protease_001
Plasmid#190063PurposeExpresses the yeast Ulp1 Sumo protease in active form as a TEV protease-cleavable MBP fusionDepositorInsertULP1_YEAST (ULP1 Budding Yeast)
Tags8X His-MBP (Maltose Binding Protein)ExpressionBacterialMutationConstruct contains residues 347 – 621PromoterT7 lacAvailable SinceJan. 30, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSPIH6
Plasmid#190676PurposeE. coli plasmid for single step cloning of SUMO-peptide-intein (SPI) fusion proteins (contains SUMO and His-tagged intein)DepositorInsertsSmall ubiquitin-like modifier
Mxe GyrA intein with C-terminal chitin binding domain and His tag
TagsChitin binding domain, His tag, and N-terminal Hi…ExpressionBacterialMutationAdded C-terminal his tag and NonePromoterT7Available SinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJBEI-6409
Plasmid#47048PurposeBglBrick plasmid (=pBbA5c-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coliDepositorInsertCodon-optimized sequences for MEV pathway expression in E. coli to produce limonene
UseSynthetic Biology; BglbrickExpressionBacterialPromoterPlacUV5Available SinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
Pseudojanin (PJ)
Plasmid#37999DepositorAvailable SinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTol2CG2-hsp70I:QFGal4-SV40pA
Plasmid#155120PurposeTol2 vector for heat-shock inducible expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis markerDepositorInserthsp70l:QFGal4 (hsp70l Zebrafish, Budding Yeast, Neurospora crassa)
UseZebrafish expressionPromoterhsp70lAvailable SinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cas9-NAT
Plasmid#64329PurposeCas9 expression cassette carried by a yeast single-copy episome plasmidDepositorInsertHuman-optimized S. pyogenes Cas9 with Clonnat marker gene expression cassette
UseCRISPRExpressionYeastAvailable SinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNESx2-EGFP-C1-PABDx2-Spo20
Plasmid#220082PurposePA biosensor with tandem Spo20-PABDs and two NES domains to aid in nuclear exportDepositorInsertSpo20 (SPO20 Budding Yeast)
TagsX. leavis map2k1.L(32-44)-GGSG-X. leavis map2k1.L…ExpressionMammalianMutationamino acids 51-91, tandem dimerPromoterCMVAvailable SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-4591NES
Plasmid#138377PurposeBooster-ROCK, a Red-Shifted Genetically Encoded FRET Biosensor for ROCK activity.DepositorInsertBooster-ROCK
ExpressionMammalianPromoterCAGAvailable SinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-4511NES
Plasmid#138375PurposeBooster-ERK, a Red-Shifted Genetically Encoded FRET Biosensor for ERK activity.DepositorInsertBooster-ERK
ExpressionMammalianPromoterCAGAvailable SinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only