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Addgene

We narrowed to 22,264 results for: fus

Showing: 5821 - 5850 of 22264 results
  1. GCN5 core-dCas9-CBP core

    Plasmid
    #179555
    Purpose
    encodes S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter
    Depositor
    Insert
    S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human CBP core (aa 1084-1701) (CREBBP, KAT2A Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
  2. CBP core-dCas9-p300 core

    Plasmid
    #179558
    Purpose
    encodes S. pyogenes dCas9 with n-terminal fusion of human CBP core (aa 1084-1701) and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoter
    Depositor
    Insert
    S. pyogenes dCas9 with n-terminal fusion of human CBP core (aa 1084-1701) & c-terminal fusion of human p300 core (aa 1048-1664) (CREBBP, EP300 Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
  3. GCN5 FL-dCas9-p300 core

    Plasmid
    #179554
    Purpose
    encodes S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoter
    Depositor
    Insert
    S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human p300 core (aa 1048-1664) (EP300, KAT2A Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
  4. pLenti-CD74-ROS1 G2032R/L2086F

    Plasmid
    #183820
    Purpose
    Express CD74-ROS1 fusion (C6;R34) harboring ROS1 G2032R/L2086F mutation in mammalian cells
    Depositor
    Inserts
    ROS Proto-Oncogene 1, Receptor Tyrosine Kinase (ROS1 Human)
    CD74 molecule (CD74 Human)
    Use
    Lentiviral
    Tags
    The fusion protein of CD74 exon 1-6 and ROS1 exon…
    Expression
    Mammalian
    Mutation
    G2032R/L2086F
    Promoter
    CMV
    Available since
    April 28, 2022
    Availability
    Academic Institutions and Nonprofits only
  5. pLenti-CD74-ROS1 S1986F/G2032R/L2086F

    Plasmid
    #183822
    Purpose
    Express CD74-ROS1 fusion (C6;R34) harboring ROS1 S1986F/G2032R/L2086F mutation in mammalian cells
    Depositor
    Inserts
    ROS Proto-Oncogene 1, Receptor Tyrosine Kinase (ROS1 Human)
    CD74 molecule (CD74 Human)
    Use
    Lentiviral
    Tags
    The fusion protein of CD74 exon 1-6 and ROS1 exon…
    Expression
    Mammalian
    Mutation
    S1986F/G2032R/L2086F
    Promoter
    CMV
    Available since
    April 28, 2022
    Availability
    Academic Institutions and Nonprofits only
  6. pLenti-CD74-ROS1 S1986F/G2032R

    Plasmid
    #183821
    Purpose
    Express CD74-ROS1 fusion (C6;R34) harboring ROS1 S1986F/G2032R mutation in mammalian cells
    Depositor
    Inserts
    ROS Proto-Oncogene 1, Receptor Tyrosine Kinase (ROS1 Human)
    CD74 molecule (CD74 Human)
    Use
    Lentiviral
    Tags
    The fusion protein of CD74 exon 1-6 and ROS1 exon…
    Expression
    Mammalian
    Mutation
    S1986F/G2032R
    Promoter
    CMV
    Available since
    April 28, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. pLenti-CD74-ROS1 S1986F/L2000V

    Plasmid
    #183818
    Purpose
    Express CD74-ROS1 fusion (C6;R34) harboring ROS1 S1986F/L2000V mutation in mammalian cells
    Depositor
    Inserts
    ROS Proto-Oncogene 1, Receptor Tyrosine Kinase (ROS1 Human)
    CD74 molecule (CD74 Human)
    Use
    Lentiviral
    Tags
    The fusion protein of CD74 exon 1-6 and ROS1 exon…
    Expression
    Mammalian
    Mutation
    S1986F/L2000V
    Promoter
    CMV
    Available since
    April 28, 2022
    Availability
    Academic Institutions and Nonprofits only
  8. pLenti-CD74-ROS1 S1986F/L2086F

    Plasmid
    #183819
    Purpose
    Express CD74-ROS1 fusion (C6;R34) harboring ROS1 S1986F/L2086F mutation in mammalian cells
    Depositor
    Inserts
    ROS Proto-Oncogene 1, Receptor Tyrosine Kinase (ROS1 Human)
    CD74 molecule (CD74 Human)
    Use
    Lentiviral
    Tags
    The fusion protein of CD74 exon 1-6 and ROS1 exon…
    Expression
    Mammalian
    Mutation
    S1986F/L2086F
    Promoter
    CMV
    Available since
    April 28, 2022
    Availability
    Academic Institutions and Nonprofits only
  9. tetO-NR2F1

    Plasmid
    #170698
    Purpose
    doxycycline-inducible overexpression of NR2F1
    Depositor
    Insert
    nuclear receptor subfamily 2 group F member 1 (NR2F1 Human)
    Use
    Lentiviral; Doxycycline inducible
    Tags
    Expression
    Mammalian
    Mutation
    deletion of 36G- please see depositor comments
    Promoter
    TRE promoter, Tet-ON
    Available since
    July 12, 2023
    Availability
    Academic Institutions and Nonprofits only
  10. Flag-miniTurbo

    Plasmid
    #124647
    Purpose
    Expresses Flag-miniTurbo fusion for proximity labeling
    Type
    Empty backbone
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 22, 2019
    Availability
    Academic Institutions and Nonprofits only
  11. Flag-TurboID

    Plasmid
    #124646
    Purpose
    Expresses Flag-TurboID fusion for proximity labeling
    Type
    Empty backbone
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 22, 2019
    Availability
    Academic Institutions and Nonprofits only
  12. pCS2+StayGold-C Cloning Vector

    Plasmid
    #208337
    Purpose
    To express a protein of interest fused to the C-terminus of StayGold in mammalian cells
    Depositor
    Type
    Empty backbone
    Use
    Tags
    StayGold
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
  13. pET28-iLOVf

    Plasmid
    #63723
    Purpose
    E coli expression plasmid to produce Thrombin cleavable His tagged iLOV fusion proteins
    Depositor
    Type
    Empty backbone
    Use
    Tags
    TEE, iLOV, 6xHis, thrombin cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 28, 2015
    Availability
    Academic Institutions and Nonprofits only
  14. pET29b-H6_Streptavidin_sfGFP

    Plasmid
    #124296
    Purpose
    His6-tagged Streptavidin GFP fusion protein
    Depositor
    Insert
    His6 tag - TEV protease site - T7 tag - Streptavidin (15-159) - superfolder GFP
    Use
    Tags
    His6 and T7 tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
  15. CRY2olig-TDP-43

    Plasmid
    #218920
    Purpose
    Expression of CRY2olig-mCherry fused to TDP-43
    Insert
    CRY2olig-mCherry tagged TDP-43
    Use
    Tags
    Expression
    Mammalian
    Mutation
    wild-type
    Promoter
    CMV
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
  16. CRY2olig-NONO

    Plasmid
    #218922
    Purpose
    Expression of CRY2olig-mCherry fused to NONO
    Insert
    CRY2olig-mCherry tagged NONO
    Use
    Tags
    Expression
    Mammalian
    Mutation
    wild-type
    Promoter
    CMV
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
  17. pMK33-GW-TurboID-V5

    Plasmid
    #196281
    Purpose
    Gateway destination vector to express C-terminal TurboID fusion under metallothionein promoter, for making stable cell lines
    Depositor
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 21, 2024
    Availability
    Academic Institutions and Nonprofits only
  18. pMK33-V5-TurboID-GW

    Plasmid
    #196282
    Purpose
    Gateway destination vector to express N-terminal TurboID fusion under metallothionein promoter, for making stable cell lines
    Depositor
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 21, 2024
    Availability
    Academic Institutions and Nonprofits only
  19. CRY2olig-PR

    Plasmid
    #218917
    Purpose
    Expression of CRY2olig-mCherry fused to a codon-optimised poly-PR sequence
    Insert
    CRY2olig-mCherry tagged poly-PR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    wild-type
    Promoter
    CMV
    Available since
    Aug. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
  20. CRY2olig-GP

    Plasmid
    #218918
    Purpose
    Expression of CRY2olig-mCherry fused to a codon-optimised poly-GP sequence
    Insert
    CRY2olig-mCherry tagged poly-GP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    wild-type
    Promoter
    CMV
    Available since
    Aug. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
  21. CRY2olig-GR

    Plasmid
    #218919
    Purpose
    Expression of CRY2olig-mCherry fused to a codon-optimised poly-GR sequence
    Insert
    CRY2olig-mCherry tagged poly-GR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    wild-type
    Promoter
    CMV
    Available since
    Aug. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
  22. mOrange in pBSDONR P4r-P2

    Plasmid
    #121529
    Purpose
    For making C-terminal mOrange fusions using multisite Gateway cloning
    Depositor
    Insert
    mOrange
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 12, 2024
    Availability
    Academic Institutions and Nonprofits only
  23. pNIC28-Bsa4-iLOVf

    Plasmid
    #63722
    Purpose
    LIC E coli expression plasmid to produce TEV cleavable His tagged iLOV fusion proteins
    Depositor
    Type
    Empty backbone
    Use
    Tags
    TEE, iLOV, 6xHis, TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 7, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 5821 - 5850 of 22264 results