We narrowed to 6,023 results for: cat.2

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pDisplay-ZIBG2

Plasmid

#169458

Purpose

cell surface localized zinc sensor with ~ 300 nM Kd

Depositor

Huiwang Ai

Article

Chen et al Anal Chem. 2019 Oct 1;91(19):12212-12219

Insert

ZIBG2

Tags

pDisplay backbone elements for export and membran…

Expression

Mammalian

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pmCherry-imp α

Plasmid

#119719

Purpose

Expresses mCherry-tagged human importinα in mammalian cells

Depositor

Kenji Sugimoto

Article

Nakagawa et al Biosci Biotechnol Biochem. 2012;76(2):38

Insert

importinα (KPNA2 Human)

Tags

mCherry

Expression

Mammalian

Mutation

contains amino acids 251-529

Promoter

CMV

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pDe-CAS9-D10A

Plasmid

#61477

Purpose

based on pDe-CAS9, encodes Cas9 D10A nickase variant

Depositor

Holger Puchta

Article

Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

Insert

Cas9-D10A

Use

CRISPR

Expression

Plant

Mutation

catalytic Asp10 changed to Ala

Promoter

PcUbi4-2

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 hML23Cm6

Plasmid

#109368

Purpose

Human melanopsin chimera with intracellular loops 2-3 and C-terminus from mGluR6

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

human Melanopsin-mGluR6 chimera (OPN4, GRM6 Human)

Tags

1D4

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCCL-GKL1RC-Short-LEUg1mKateg2GKL2ORF10UCS

Plasmid

#52345

Purpose

Integration cassette to create p1-Short-Leu-mKate with p2 ORF10 UCS>mKate

Depositor

Chang Liu

Article

Ravikumar et al Nat Chem Biol. 2014 Mar;10(3):175-7. doi

Insert

mKate2

Use

P1 integration vector

Expression

Bacterial

Mutation

changed V2H

Promoter

pGKL2 ORF10 UCS

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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hsp70:Arl13b-mKate2

Plasmid

#105964

Purpose

heat shock inducible transgenesis, primary cillium marker

Depositor

Caren Norden

Article

Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

Insert

Arl13b (Arl13b Mouse)

Tags

mKate2

Expression

Bacterial

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pAP05

Plasmid

#186261

Purpose

sfGFP under light light responsive PEL222 promoter and EL222 under constitutively active BBa_J2305 promoter

Depositor

Yongku Cho

Article

Pirhanov et al ACS Synth Biol. 2021 Feb 19;10(2):345-35

Inserts

sfGFP
EL222 (ELI_04755 Erythrobacter litoralis)

Expression

Bacterial

Promoter

BBa_23105 (GGCTAGCTCAGTCCTAGGTACTATGCTAGC) and PE…

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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Cas12f-GE ver4.0

Plasmid

#176543

Purpose

Expresses Cas12f-GE in mammalian cells

Depositor

Yong-Sam Kim

Article

Kim et al Nat Biotechnol. 2021 Sep 2. pii: 10.1038

Inserts

Cas12f-GE ver4.0
Cas12f-GE ver4.0

Expression

Mammalian

Promoter

U6 and chicken β-actin

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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Yellow Cameleon-Nano15/pBig

Plasmid

#51962

Purpose

Expression of Yellow Cameleon-Nano15, an ultrasensitive Ca2+ indicator with Kd = 15 nM, in Dictyostelium

Depositor

Takeharu Nagai

Article

Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

Insert

Yellow Cameleon-Nano15

Expression

Bacterial

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nsp13

Plasmid

#157712

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp13 (ORF1ab SARS-CoV-2)

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-Nsp10

Plasmid

#157706

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp10 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp10 (ORF1ab SARS-CoV-2)

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDA093

Plasmid

#131390

Purpose

generation of Tet-On RPE

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

ORFeus

Expression

Mammalian

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pDA095

Plasmid

#131391

Purpose

generation of Tet-On RPE

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

ORFeus

Expression

Mammalian

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pCCL-ReDNAP (Y427A)

Plasmid

#52346

Purpose

CEN6/ARS4 plasmid, REV1 promoter>Recoded TP-DNAP1 (Y427A)

Depositor

Chang Liu

Article

Ravikumar et al Nat Chem Biol. 2014 Mar;10(3):175-7. doi

Insert

Recoded TP-DNAP

Expression

Bacterial and Yeast

Promoter

REV1

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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FASTHDR-Cterm-mRuby3-Zeo

Plasmid

#167206

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mRuby3 and selection with Zeocin

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

mRuby3

Expression

Mammalian

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD-K-GECO1

Plasmid

#105864

Purpose

Bacterial expression plasmid for red fluorescent Ca2+ indicator K-GECO1

Depositor

Robert Campbell

Article

Shen et al BMC Biol. 2018 Jan 16;16(1):9. doi: 10.1

Insert

K-GECO1

Tags

6-His Tag

Expression

Bacterial

Available Since

April 27, 2018

Availability

Academic Institutions and Nonprofits only

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ACE2 g3

Plasmid

#153013

Purpose

A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing mCherry

Depositor

Jason Sheltzer

Insert

ACE2 (ACE2 Human)

Use

CRISPR and Lentiviral

Tags

mCherry

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nsp10-HF

Plasmid

#157708

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp10 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp10-HF (ORF1ab SARS-CoV-2)

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-FH-Nsp10

Plasmid

#157707

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp10 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp10 (ORF1ab SARS-CoV-2)

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-FH-Nsp9

Plasmid

#157704

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp9 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp9 (ORF1ab SARS-CoV-2)

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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