We narrowed to 162,676 results for: addgene

1. N-terminal Halo-Ku70

   Plasmid

   #207547

   Purpose

   Homologous recombination donor to insert halo tag at the N terminal of the endogenous Ku70 locus.

   Depositor

   Jens Schmidt

   Article

   Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

   Insert

   HaloTag with flanked by human Ku70 locus sequences
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. aTY171_pPGK1_iRFP_WPRE

   Plasmid

   #225351

   Purpose

   Lentiviral vector for iRFP expression

   Depositor

   Wendell Lim

   Article

   Yamada et al Cell. 2024 Dec 11:S0092-8674(24)01323-0.

   Insert

   iRFP670
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. aTY173_pPGK1_mTagBFP2_WPRE

   Plasmid

   #225352

   Purpose

   Lentiviral vector for BFP expression

   Depositor

   Wendell Lim

   Article

   Yamada et al Cell. 2024 Dec 11:S0092-8674(24)01323-0.

   Insert

   BFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Talin-1 WT

   Plasmid

   #201798

   Purpose

   Expresses Talin-1 protein in MAC-Tag-C backbone

   Depositor

   Vesa Hytönen

   Article

   Turkki et al Sci Rep. 2024 Aug 27;14(1):19862. doi: 1

   Insert

   Talin-1 (Tln1 Mouse)
   

   Use

   Tags

   BirA, HA, strepIII

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. CROPseq-Puro-2xMS2

   Plasmid

   #212627

   Purpose

   MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles

   Depositor

   Paul Blainey

   Article

   Najia et al bioRxiv 2024.10.01.616098

   Insert

   EF1a-PuroR-2xMS2-WPRE-hU6-sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. CROPseq-Puro-12xMS2

   Plasmid

   #212628

   Purpose

   MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles

   Depositor

   Paul Blainey

   Article

   Najia et al bioRxiv 2024.10.01.616098

   Insert

   EF1a-PuroR-12xMS2-WPRE-hU6-sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. CROPseq-Puro-4xMS2

   Plasmid

   #212626

   Purpose

   MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles

   Depositor

   Paul Blainey

   Article

   Najia et al bioRxiv 2024.10.01.616098

   Insert

   EF1a-PuroR-4xMS2-WPRE-hU6-sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pZB573_pB1H1_T7-RNAP_LARP-I5CHO_1

   Plasmid

   #228508

   Purpose

   Bacterial Hybrid Driver Plasmid

   Depositor

   Timothy Whitehead

   Article

   Baumer et al ACS Synth Biol. 2024 Dec 20;13(12):4165-

   Insert

   T7 RNAP, LARP-I5CHO (#1)
   

   Use

   Synthetic Biology

   Tags

   Umu Degron, GTG-start codon

   Expression

   Mutation

   R378K; S430P, N433T, S633P, F849I, F880Y; W727G, …

   Promoter

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pZB578_pB1H1_T7-RNAP_LARP-I

   Plasmid

   #228507

   Purpose

   Bacterial Hybrid Driver Plasmid

   Depositor

   Timothy Whitehead

   Article

   Baumer et al ACS Synth Biol. 2024 Dec 20;13(12):4165-

   Insert

   T7 RNAP, LARP-I
   

   Use

   Synthetic Biology

   Tags

   Umu Degron, GTG-start codon

   Expression

   Mutation

   R378K; S430P, N433T, S633P, F849I, F880Y; W727G, …

   Promoter

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pX458-PLK4

    Plasmid

    #227310

    Purpose

    Expresses SpCas9 and a sgRNA targeting the C-terminus of PLK4 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of PLK4 (PLK4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p8270 LentiCRISPR v2 hygro sgSIGIRR-3

    Plasmid

    #193979

    Purpose

    Expression of spCas9 and sgRNA targeting SIGIRR

    Depositor

    Elizabeth White

    Article

    Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

    Insert

    spCas9 and sgRNA targeting SIGIRR (SIGIRR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pU6-tevopreq1-FXR1-G266E

    Plasmid

    #225482

    Purpose

    Express epegRNA for FXR1

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    epegRNA for editing FXR1-G266E (FXR1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LsgRNA-FXR1-KH1-gRNA

    Plasmid

    #225483

    Purpose

    Express gRNA for FXR1

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    gRNA for editing FXR1-KH1 (FXR1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. BPK1520-FXR1-N202S-gRNA

    Plasmid

    #225481

    Purpose

    Express gRNA for FXR1

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    gRNA for editing FXR1-N202S (FXR1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLVUT-eC3GFP

    Plasmid

    #225485

    Purpose

    Tet-on lentiviral vector for controlled expression of C. botulinum exoenzyme C3 transferase and EGFP

    Depositor

    Robert Gross

    Article

    Gutekunst et al Neuroscience. 2016 Dec 17;339:308-318. d

    Insert

    Exoenzyme C3 transferase
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Amino acids 30-251, See Depositor Comments

    Promoter

    Human ubiquitin C

    Available since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pST39-Mega'

    Plasmid

    #216877

    Purpose

    Derived from the plasmid for Mega GVs, pST39-pNL29 (Addgene #91696), but the overlap between GvpS and GvpK has been resolved.

    Depositor

    George Lu

    Article

    Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

    Insert

    Derived from Mega, and GvpS and GvpK overlap has been resolved
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p8391 LentiCRISPRv2 Neo sgPTPN14-1

    Plasmid

    #221651

    Purpose

    Expression of sgRNA targeting the locus of human PTPN14

    Depositor

    Elizabeth White

    Article

    Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

    Insert

    sgPTPN14-1 (PTPN14 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p8392 LentiCRISPRv2 Neo sgPTPN14-3

    Plasmid

    #221652

    Purpose

    Expression of sgRNA targeting the locus of human PTPN14

    Depositor

    Elizabeth White

    Article

    Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

    Insert

    sgPTPN14-3 (PTPN14 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pNLS-VP16-NIR-Fb(718)

    Plasmid

    #220745

    Purpose

    NLS-VP16-NIR-Fb(718) consrtuct expression in mammalian cells.

    Depositor

    Vladislav Verkhusha

    Article

    Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

    Insert

    NIR-Fb(718)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    NLS-VP16

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-CAG-NIR-FbLAG16

    Plasmid

    #220749

    Purpose

    NIR-FbLAG16 expression in mammalian cells. pAAV vector.

    Depositor

    Vladislav Verkhusha

    Article

    Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

    Insert

    NIR-FbLAG16
    

    Use

    AAV and Affinity Reagent/ Antibody

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge