We narrowed to 22,070 results for: his

1. pETSUMO-TtAgo

   Plasmid

   #210191

   Purpose

   Bacterial expression of the His- and SUMO-tagged Argonaute protein of Thermus thermophilus

   Depositor

   Phillip Zamore

   Article

   Smith et al Nat Commun. 2019 Jan 17;10(1):272. doi:

   Insert

   TtAgo (TT_P0026 Thermus thermophilus)
   

   Use

   Tags

   6x His tag and SUMO tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQE30-mBFP

   Plasmid

   #112682

   Purpose

   Expression of NADPH-dependent blue fluorescent protein

   Depositor

   Geun-Joong Kim

   Article

   Hwang et al Biochem Biophys Res Commun. 2012 Mar 23;

   Insert

   His-mBFP
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-cMSP1E3D1

   Plasmid

   #199772

   Purpose

   Expresses split-intein fused MSP1E3D1 for circularization during expression (external His-Tag)

   Depositor

   Franz Hagn

   Article

   Daniilidis et al J Mol Biol. 2022 Dec 30;434(24):167861.

   Insert

   cMSP1E3D1
   

   Use

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   Extended cMSP1D1; external His-Tags flanking the …

   Promoter

   Available since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPD304 VPR-ZF43

   Plasmid

   #138840

   Purpose

   Constitutive expression of VPR-ZF43 under the CMV promoter.

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   VPR-ZF43
   

   Use

   Synthetic Biology

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPD100 VP16-ZF43

   Plasmid

   #138761

   Purpose

   Constitutive expression of VP16-ZF43 under the CMV promoter.

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   VP16-ZF43
   

   Use

   Synthetic Biology

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pPD536 VP16-ZF150

   Plasmid

   #138769

   Purpose

   Constitutive expression of VP16-ZF150 under the CMV promoter.

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   VP16-ZF150
   

   Use

   Synthetic Biology

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPD538 VP16-ZF172

   Plasmid

   #138772

   Purpose

   Constitutive expression of VP16-ZF172 under the CMV promoter.

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   VP16-ZF172
   

   Use

   Synthetic Biology

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPD539 VP16-ZF173

   Plasmid

   #138773

   Purpose

   Constitutive expression of VP16-ZF173 under the CMV promoter.

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   VP16-ZF173
   

   Use

   Synthetic Biology

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPD526 VP16-ZF13

   Plasmid

   #138755

   Purpose

   Constitutive expression of VP16-ZF13 under the CMV promoter.

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   VP16-ZF13
   

   Use

   Synthetic Biology

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Parp14-c011

    Plasmid

    #173098

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    PARP14 (PARP14 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    K791-F978

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-cMSP1D1

    Plasmid

    #199768

    Purpose

    Expresses split-intein fused MSP1D1 for circularization during expression (external His-Tag)

    Depositor

    Franz Hagn

    Article

    Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

    Insert

    cMSP1D1
    

    Use

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    external His-Tags flanking the C and N termini

    Promoter

    Available since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1 Orai2

    Plasmid

    #16369

    Depositor

    Anjana Rao

    Article

    Gwack et al J Biol Chem. 2007 Jun 1. 282(22):16232-4

    Insert

    Orai2 (ORAI2 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJCC_083 Cas12i1

    Plasmid

    #171670

    Purpose

    For bacterial expression of Cas12i1 with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

    Insert

    Cas12i1
    

    Use

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJCC_039 FnCas12a

    Plasmid

    #171666

    Purpose

    For bacterial expression of FnCas12a with a C-terminal sorting motif and His tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

    Insert

    FnCas12a
    

    Use

    Tags

    6X His and sortase A sorting motif

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBN154

    Plasmid

    #86718

    Purpose

    FLP/Frt dual color reporter for C. elegans

    Depositor

    Peter Askjaer

    Article

    Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

    Insert

    Phsp16.41::FRT::mCh::his-58::FRT::gfp::his-58
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET32-sLatherin

    Plasmid

    #61685

    Purpose

    bacterial expression of the horse sweat/saliva protein Latherin as a His-tagged thioredoxin fusion

    Depositor

    Brian Smith

    Article

    Vance et al J R Soc Interface. 2013 Jun 19;10(85):20

    Insert

    Latherin (LATH horse)
    

    Use

    Tags

    HIS and Trx-Tag thioredoxin protein

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    T7

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pWaldo-GFPd_PepTSt

    Plasmid

    #58333

    Purpose

    Expresses the POT family transporter from Streptococcus thermophilus in E. coli as a C-terminally tagged GFP his tagged protein

    Depositor

    Simon Newstead

    Article

    Solcan et al EMBO J. 2012 Aug 15;31(16):3411-21. doi:

    Insert

    PepTSt
    

    Use

    Tags

    GFP-His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pTD-C_eYFPTwinStrep

    Plasmid

    #45942

    Purpose

    expresses E.coli codon optimized EYFP-Twin-Strep fusion protein

    Depositor

    Thorben Dammeyer

    Article

    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

    Insert

    synthetic eYFP (codon usage adapted to E.coli K12)
    

    Use

    Tags

    Twin-Strep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    lacIq/Ptrc

    Available since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPD1028 ZF43x6-Compact(SV40Min)_EYFP

    Plasmid

    #138912

    Purpose

    EYFP reporter vector for the ZF43x6-Compact(overlapping ZF37) promoter with a SV40 minimal promoter

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF43x6-Compact(SV40min)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    ZF43x6-Compact(SV40min)

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-cMSPdH5

    Plasmid

    #199771

    Purpose

    Expresses split-intein fused MSPdH5 for circularization during expression (external His-Tag)

    Depositor

    Franz Hagn

    Article

    Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

    Insert

    cMSPdH5
    

    Use

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    external His-Tags flanking the C and N termini

    Promoter

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28-Rsn2

    Plasmid

    #61686

    Purpose

    bacterial expression of ranaspumin 2 as a His-tagged fusion protein

    Depositor

    Brian Smith

    Article

    Mackenzie et al Biophys J. 2009 Jun 17;96(12):4984-92. d

    Insert

    Ranaspumin-2
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. VV001: WT Arch-3 in pET28b

    Plasmid

    #58487

    Purpose

    Expresses WT Archaerhodopsin-3 (with 6x C-terminal His tag) in E. coli

    Depositor

    Adam Cohen

    Article

    Maclaurin et al Proc Natl Acad Sci U S A. 2013 Apr 9;110

    Insert

    Archaerhodopsin-3 (with C-terminal His tag)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pPD005 pcDNA Golden Gate

    Plasmid

    #138749

    Purpose

    Vector used to express most COMET components, modified from pcDNA3.1/HygroR(+) for BsaI/BpiI Golden Gate compatibility and to lack HygroR.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. PCRT7 tetR pLtetO MBP-MEK1 XX Amp

    Plasmid

    #53225

    Purpose

    human MEK1 with MBP and HIS tags, with Ser218 and Ser222 changed to amber codons, for use in phosphoprotein synthesis

    Depositor

    Jesse Rinehart

    Insert

    MEK1 (MAP2K1 Human)
    

    Use

    Phosphoprotein synthesis

    Tags

    HIS and MBP

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli, both codons for Ser21…

    Promoter

    pLtetO

    Available since

    May 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEQ1007

    Plasmid

    #83938

    Purpose

    express human cytomegalovirus IRS1 with a C-terminal 6X his tag in eukaryotic cells

    Depositor

    Adam Geballe

    Article

    Hakki et al J Virol. 2006 Dec;80(23):11817-26. Epub

    Insert

    IRS1
    

    Use

    Tags

    6xHIS

    Expression

    Mammalian

    Mutation

    R833L in IRS1 (please see depositor comment)

    Promoter

    CMV

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pPD1029 ZF43x6-Compact(CMVMin)_EYFP

    Plasmid

    #138913

    Purpose

    EYFP reporter vector for the ZF43x6-Compact(overlapping ZF37) promoter with a CMV minimal promoter

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF43x6-Compact(CMVmin)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    ZF43x6-Compact(CMVmin)

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pPD057 VP64-ZF37

    Plasmid

    #138834

    Purpose

    Constitutive expression of VP64-ZF37 under the CMV promoter.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    VP64-ZF37
    

    Use

    Synthetic Biology

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCoofy48

    Plasmid

    #55186

    Purpose

    Bacterial expression vector for parallel SLIC cloning containing N-terminal 6x His and GST tags. C-terminal tags can be added during SLIC.

    Depositor

    Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Use

    Tags

    6x His, GST, His10-Stop-OneStrep-Stop-S-tag-Stop-…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pPD391 VPR-ZF37

    Plasmid

    #138839

    Purpose

    Constitutive expression of VPR-ZF37 under the CMV promoter.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    VPR-ZF37
    

    Use

    Synthetic Biology

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pPD159 dsRedExpress2-ZFi43

    Plasmid

    #138799

    Purpose

    Constitutive expression of dsRedExpress2-ZFi43 under the CMV promoter.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    dsRedExpress2-ZFi43
    

    Use

    Synthetic Biology

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge