We narrowed to 13,333 results for: Mpl;

1. pMaCTag-P33

   Plasmid

   #120018

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with FLAG

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   FLAG
   

   Use

   Pcr template

   Tags

   FLAG

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDGE6

   Plasmid

   #79476

   Purpose

   sgRNA shuttle vector; module 1E

   Depositor

   Johannes Stuttmann

   Article

   Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

   Insert

   pAtU6-ccdB-sgRNA (ccdB(letD) )
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   BsaI site eliminated

   Available Since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-EGFP-C-IFT70B

   Plasmid

   #218729

   Purpose

   Expresses N-terminally EGFP-tagged IFT70B in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Takei et al Biol Open. 2018 Apr 30;7(5). pii: bio.03

   Insert

   IFT70B (TTC30B Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDF0386 pcmv-hudiscas7-11-s1006-gggs-d1221

   Plasmid

   #186983

   Purpose

   mammalian expression of cas7-11-s1006-gggs-d1221

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

   Insert

   cas7-11-s1006-gggs-d122
   

   Expression

   Mammalian

   Mutation

   s1006-gggs-d1221 deletion

   Available Since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCI-neo VPS51-13Myc

   Plasmid

   #175094

   Purpose

   13Myc tagged VPS51 is expressed in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Gershlick et al Hum Mol Genet. 2019 May 1;28(9):1548-156

   Insert

   VPS51 (VPS51 Human)
   

   Tags

   13Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-5X1 Flag RbBP5

   Plasmid

   #15551

   Depositor

   Kai Ge

   Article

   Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

   Insert

   RbBP5 (RBBP5 Human)
   

   Tags

   Flag and GST

   Expression

   Bacterial

   Available Since

   Aug. 16, 2007

   Availability

   Academic Institutions and Nonprofits only

7. modRNAc0-Cas9

   Plasmid

   #170180

   Purpose

   IVT template plasmid for making modified Cas9 RNA

   Depositor

   Xiaojun Lian

   Article

   Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290

   Insert

   Cas9
   

   Use

   Ivt template plasmid

   Promoter

   T7 promoter

   Available Since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. LAMP1-ABKAR

   Plasmid

   #65068

   Purpose

   Expression of ABKAR biosensor targeted to the lysozome

   Depositor

   Jin Zhang, Takanari Inoue

   Article

   Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

   Insert

   ABKAR biosensor
   

   Tags

   Lamp1

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pBMN mCherry-TAX1BP1(ΔUBD)

   Plasmid

   #119689

   Purpose

   For mammalian expression of TAX1BP1 without its UBD

   Depositor

   Michael Lazarou

   Article

   Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

   Insert

   TAX1BP1 (TAX1BP1 Human)
   

   Use

   Retroviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   UBD deletion (1-755)

   Available Since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. PX429 SpCas9 N863A nickase

    Plasmid

    #68706

    Purpose

    SpCas9 N863A nickase

    Depositor

    Feng Zhang

    Article

    Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10

    Insert

    SpCas9 N863A nicking mutant
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. Nuc-ABKAR

    Plasmid

    #61511

    Purpose

    Expression of nuclear AMPK biosensor

    Depositor

    Jin Zhang, Takanari Inoue

    Article

    Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

    Insert

    AMPK Biosensor
    

    Tags

    NLS (see comments)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

12. CCR5-donor-MCS

    Plasmid

    #192269

    Purpose

    Donor template for CRISPR Genome Editing

    Depositor

    Shantanu Chowdhury

    Article

    Sharma et al Cell Rep. 2021 May 18;35(7):109154. doi:

    Insert

    MCS within donor template
    

    Use

    CRISPR

    Available Since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTS103 pHAGE2 CMVtetO2 MCS-22xGS-GFP

    Plasmid

    #199356

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally GFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pXWJ109Hg-gfp

    Plasmid

    #123148

    Purpose

    A mercury sensor with optimized intracellular receptor density. pSB3K3 carrying J109-32merR-t-PmerT-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J109-32merR-t-PmerT-30gfp-t
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J109, PmerT

    Available Since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMaCTag-Z22

    Plasmid

    #120065

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with SNAP

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    SNAP
    

    Use

    Pcr template

    Tags

    SNAP

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSPlit-OC-hDib1

    Plasmid

    #58789

    Purpose

    Express hDib1-LucC fusion protein in mammalian cells

    Depositor

    Hiroyoshi Ariga

    Article

    Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

    Insert

    hDib1 (TXNL4A Human)
    

    Tags

    Flag and Luciferase (394-550aa)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

17. Integrin beta1(1-455)-tev-BHCys

    Plasmid

    #27315

    Depositor

    Timothy Springer

    Article

    Takagi et al EMBO J. 2003 Sep 15. 22(18):4607-15.

    Insert

    integrin beta1 (ITGB1 Human)
    

    Expression

    Mammalian

    Mutation

    beta1(1-455)+GG+TEV+GGKN+BHCys+TG+His6

    Available Since

    April 8, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pMaCTag-Z34

    Plasmid

    #120062

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 3xFLAG

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    3xFLAG
    

    Use

    Pcr template

    Tags

    3xFLAG

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMaCTag-P27

    Plasmid

    #120038

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 1xHA

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    1xHA
    

    Use

    Pcr template

    Tags

    1xHA

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAW-AlphaLobe

    Plasmid

    #113255

    Purpose

    E. coli expression vector for His-MBP-Cas9 Alpha-Helical Lobe

    Depositor

    Jennifer Doudna

    Article

    Wright et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    S. pyogenes Cas9 alpha-helical lobe
    

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    residues 56–714; See paper for description

    Promoter

    T7

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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