We narrowed to 9,007 results for: mel

1. pPB-cT3G-cERP2-PRDM1

   Plasmid

   #222924

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of PRDM1

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2022.07.11.499564

   Insert

   PRDM1 (PRDM1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pINDUCER20_mStrawberry

   Plasmid

   #161734

   Purpose

   Doxycycline-inducible expression of mStrawberry

   Depositor

   Alec Kimmelman

   Article

   Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:

   Insert

   mStrawberry
   

   Use

   Lentiviral; Destinatioin vector for gateway cloni…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. HA-USPL1-pcDNA3.1

   Plasmid

   #85760

   Purpose

   mammalian expression of full length HA tagged USPL1

   Depositor

   Frauke Melchior

   Article

   Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

   Insert

   USPL1, full length (USPL1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKD001 pME minprom_EGFP

   Plasmid

   #195955

   Purpose

   Gateway pME fluorophore

   Depositor

   Christian Mosimann

   Article

   Prummel et al Nat Commun. 2019 Aug 26;10(1):3857. doi:

   Insert

   mouse beta-globin minimal promoter EGFP
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28b-hUba2-His

   Plasmid

   #53117

   Purpose

   expression of full length human Uba2 with Cterminal His-tag in E.coli

   Depositor

   Frauke Melchior

   Article

   Werner et al Methods Mol Biol. 2009;497:187-99. doi:

   Insert

   UBA2 (UBA2 Human)
   

   Use

   Tags

   His and T7 tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28 MBP NAAEF-AMPKa1 (13-476,529-550)-b2(76-272)-g1(24-327)

   Plasmid

   #177850

   Purpose

   Bacterial Expression of AMPK

   Depositor

   Karsten Melcher

   Article

   Yan et al Science. 2021 Jul 23;373(6553):413-419.

   Insert

   AMPK (a1b2g1)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28C-mCherry-DDX4LCD

   Plasmid

   #204408

   Purpose

   E. coli expression of DDX4 low-complexity domain (1-236) with an N-terminal mCherry tag

   Depositor

   Samie Jaffrey

   Article

   Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

   Insert

   DDX4(1-236) (DDX4 Human)
   

   Use

   Tags

   6x-His; mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28C-mCherry-53BP1LCD

   Plasmid

   #204407

   Purpose

   E. coli expression of the 53BP1 low-complexity domain (1208-1977) with an N-terminal mCherry tag

   Depositor

   Samie Jaffrey

   Article

   Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

   Insert

   53BP1(1208-1977) (TP53BP1 Human)
   

   Use

   Tags

   6x-His; mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPB-cT3G-cERH-ZNF281

   Plasmid

   #222923

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of ZNF281

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2022.07.11.499564

   Insert

   ZNF281 (ZNF281 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV CAG-DO-TC66T-mCherry

    Plasmid

    #175178

    Purpose

    Cre-off TVA-mCherry fusion protein with 66T mutation

    Depositor

    Bernardo Sabatini

    Article

    Melzer et al Cell. 2021 Oct 28;184(22):5622-5634.e25.

    Insert

    TVA
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    E66T

    Promoter

    CAG

    Available since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. "AND-gate" receiver plasmid

    Plasmid

    #127676

    Purpose

    Encodes the transcriptional activator LuxR and GFP. LuxR has an pLlacO-1 promoter and the GFP has a pLux promoter.

    Depositor

    Friedrich Simmel

    Article

    Schwarz-Schilling et al Integr Biol (Camb). 2016 Apr 18;8(4):564

    Insert

    LuxR, GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLlacO-1 for LuxR, pLux for GFP

    Available since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHDR2_DAZL-T2A-mGreenLantern-PuroTK

    Plasmid

    #222917

    Purpose

    Homology directed repair template for knocking in mGreenLantern reporter to DAZL.

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2022.07.11.499564

    Insert

    mGreenLantern
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAd MafA-I-nGFP

    Plasmid

    #19412

    Depositor

    Douglas Melton

    Article

    Zhou et al Nature. 2008 Oct 2;455(7213):627-32. doi

    Insert

    v-maf musculoaponeurotic fibrosarcoma oncogene family, protein A (avian) (Mafa Mouse)
    

    Use

    Adenoviral; Entry vector

    Tags

    GFP and IRES

    Expression

    Mutation

    Promoter

    Available since

    Nov. 12, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pET23a-His-hRanGAP1tail

    Plasmid

    #53139

    Purpose

    E.coli expression of Cterminal domain of human RanGAP1 with Nterminal His-tag

    Depositor

    Frauke Melchior

    Article

    Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

    Insert

    RanGAP1 (RANGAP1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    C-terminal fragment (amino acids 398-587)

    Promoter

    T7

    Available since

    May 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. MiniCoopR U6:gRNA p53, mitfa:Cas9

    Plasmid

    #118841

    Purpose

    Targets tp53 and expresses zebrafish mitfa specifically in zebrafish melanocytes

    Depositor

    Leonard Zon

    Article

    Ablain et al Science. 2018 Nov 1. pii: science.aau650

    Insert

    tp53 gRNA (tp53 Zebrafish)
    

    Use

    CRISPR; Tol2

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEF1a-2xSV40_NLS-NLuc

    Plasmid

    #135953

    Purpose

    Expresses the NanoLuciferase targeted to the nucleus in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    NanoLuciferase
    

    Use

    Tags

    2xSV40-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHDR_NANOS3-T2A-mVenus-PuroTK

    Plasmid

    #222903

    Purpose

    Homology directed repair template for knocking in mVenus reporter to NANOS3.

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2022.07.11.499564

    Insert

    mVenus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CBA-DIO-GCaMP6s-P2A-mBeRFP-WPRE-pA

    Plasmid

    #175177

    Purpose

    Cre-dependent GCaMP6s and mBeRFP expression

    Depositor

    Bernardo Sabatini

    Article

    Melzer et al Cell. 2021 Oct 28;184(22):5622-5634.e25.

    Insert

    GCaMP6s
    

    Use

    AAV

    Tags

    P2A-mBeRFP

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-EF1a-double floxed-Aldh1a1-HA-WPRE-HGHpA

    Plasmid

    #184633

    Purpose

    Expresses Aldh1a1 fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashion

    Depositor

    Nicolas Tritsch

    Article

    Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

    Insert

    Aldh1a1 (Aldh1a1 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. dmBACCS2VL-IRES-dOrai

    Plasmid

    #67629

    Purpose

    Mammalian expression of dmBACCS2VL, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, and dOrai

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    dmBACCS2VL-IRES-dOrai
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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