-
Plasmid#39030PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertpolybromo 1 [isoform a] (PBRM1 Human)
UseTagsHis TevExpressionBacterialMutationcodon-optimizedPromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
p-dCas9-SET7-H297G-Hygro
Plasmid#104415Purposetransient expression of dCas9-dSET7 fusion proteinDepositorInsertdSET7
UseCRISPRTagsExpressionMammalianMutationH297GPromoterCMV promoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQCXIpuro-ITGA5
Plasmid#134764Purposea retroviral vector to express integrin alpha5DepositorInsertITGA5 (ITGA5 )
UseRetroviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG471 Lyn11-iFAST
Plasmid#130817PurposeExpresses Lyn11-iFAST in mammalian cells (inner plasma membrane labeling)DepositorInsertLyn11-iFAST
UseTagsmyc-tagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
-
GSK-3beta-his (Xenopus)/pCS2+
Plasmid#16335DepositorInsertglycogen synthase kinase-3beta (gsk3b.L Frog)
UseVertebrate expressionTags6xHisExpressionMutationXenopus GSK-3 with 6X his tagPromoterAvailable sinceDec. 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
-
-
pLEX307-TMPRSS4-blast
Plasmid#158473PurposeConstitutive lentiviral expression of human TMPRSS4.DepositorInsertTMPRSS4 (TMPRSS4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1Q1
Plasmid#97326PurposeNanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional Q residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.DepositorInsertMSP1D1Q1
UseTags6xHis - TEVExpressionBacterialMutationAddition of Q residue after T25PromoterT7Available sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
-
pBCSK_LEU2_I
Plasmid#109060Purposeyeast shuttle vector for insertion into yeast chromosomes in pBCSK backbone. LoxP sites upstream of LEU2. For teaching purposesDepositorInsertLEU2 (LEU2 Zygosaccharomyces rouxii, Budding Yeast)
UseCre/LoxTagsExpressionBacterial and YeastMutationPromoterLEU2Available sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
-
-
pENTR hH1-ARRET
Plasmid#111382PurposeARRET with approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences under control of hH1 promoter and terminatorDepositorInserthH1-ARRET
UseGateway entry cloningTagsExpressionMutationPromoterhH1Available sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
-
pTALE64G
Plasmid#35188PurposeGolden Gate Compatible TALE Transcription Factor using VP64 Domain with T2A-GFP additionDepositorInsertTAL Effector-VP64-T2A-eGFP
UseTALENTagsExpressionMammalianMutation+63 C Terminus, VP64 Domain, T2A-GFPPromoterCMVAvailable sinceDec. 12, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
-
TALEN_C63
Plasmid#40788PurposeGolden Gate Compatible TALEN Construct for site specific modification of genome, +63 C Terminus, Codon Optimized FokIDepositorInsertTranscription Activator Like Effector Nuclease
UseTALENTagsExpressionMammalianMutation+63 C Terminus, Codon Optimized FokI EndonucleasePromoterCMVAvailable sinceDec. 12, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLenti4/V5-DEST-P85(DKRMNS560del)
Plasmid#40225DepositorInsertP85(DKRMNS560del) (PIK3R1 Human)
UseLentiviralTagsFlagExpressionMammalianMutationdeleted amino acids 560-565PromoterCMVAvailable sinceOct. 11, 2012AvailabilityAcademic Institutions and Nonprofits only