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We narrowed to 5,294 results for: codon optimized

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Showing: 3921 - 3940 of 5294 results

  4. pCC_06 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9NG-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139091
    Purpose
    Expresses human codon-optimized inactive SpCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dSpCas9-NG-VPR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, L1111R, D1135V,G1218R, E1219F, A1322…
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139091-plasmid-map-sequence-id-273888

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  7. Lenti-EFS-BirAopt-GSQ-RBXN-P2A-BLAST

    Plasmid
    #208048
    Purpose
    Enables constitutive expression of BirAopt alone; RBXN represents a EcoR1-BsiW1-Xba1-Not1 polylinker; to perform bioE3; selection with blasticidin
    Depositor
    Rosa Barrio, James Sutherland
    Article
    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:
    Insert
    BirAopt
    Use
    Lentiviral
    Mutation
    BirAopt is human-codon-optimized BirA (PMID 15707…
    Promoter
    EFS
    Available Since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208048-plasmid-map-sequence-id-410202

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  8. eiCBE

    Plasmid
    #227148
    Purpose
    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with two copies of UGI and hA3A* variant driven by CMV promoter
    Depositor
    Dali Li
    Article
    Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e
    Insert
    bpNLS-hA3A*-linker-nOgeuIscB-v3-nucleoplasmin NLS-2XUGI-bpNLS
    Mutation
    D96R/E84R/V159R/H339A
    Promoter
    CMV
    Available Since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    227148-plasmid-map-sequence-id-453981

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  9. p28-2iDMD-puro

    Plasmid
    #88895
    Purpose
    28°C full-length DMD luc-puro-red plasmid. Co-expresses human DMD, firefly luciferase, puroR and mCherry in mammalian cells. Plasmid carries phiC31 and Bxb1 attB sites, but *cannot* be grown at 37°C.
    Depositor
    Michele Calos
    Article
    Farruggio et al Biotechnol J. 2017 Jan 31. doi: 10.1002/
    Inserts
    Dystrophin (DMD Human)
    luciferase
    puromycin resistance enzyme
    mCherry
    Expression
    Mammalian
    Mutation
    codon optimized
    Available Since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    88895-plasmid-map-sequence-id-451851

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  11. lentiCRISPR - EGFP sgRNA 1

    Plasmid
    #51760
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 1
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EFS and U6
    Available Since
    March 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    51760-plasmid-map-sequence-id-392672

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  15. lentiCRISPR - EGFP sgRNA 2

    Plasmid
    #51761
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 2
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EFS and U6
    Available Since
    March 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    51761-plasmid-map-sequence-id-79377

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  16. pETM33_Nsp16

    Plasmid
    #156482
    Purpose
    Bacterial expression of Sars-CoV2 Nsp16 protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp16 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156482-plasmid-map-sequence-id-307736

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Showing: 3921 - 3940 of 5294 results