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pRCA422 - pHR-UCOE-SFFV-VPH-dcas9-p2a-mCherry

Plasmid

#238165

Purpose

Express VPH-dCas9-P2A-mCherry in mammalian cells. Lentiviral

Depositor

Thomas Norman

Publication

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Insert

VPH-dCas9-P2A-mCherry

Use

CRISPR and Lentiviral

Tags

mCherry

Available since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pLGR002 LGR Cas9 guide vector

Plasmid

#188320

Purpose

Universal backbone for CRISPR/Cas9 sgRNA expression

Depositor

Laralynne Przybyla

Publication

Heo et al Genome Biol. 2024 Jan 19;25(1):25. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

PuroR-T2A-mTagBFP2

Expression

Mammalian

Promoter

pU6 5'CMV and EF1-alpha

Available since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHis-hPim1

Plasmid

#100722

Purpose

Expression of human Pim1 kinase domain in E.coli

Depositor

Byung Il Lee

Publication

Lee et al PLoS One. 2013 Jul 31;8(7):e70358. doi:

Insert

Pim1 (PIM1 Human)

Tags

His

Expression

Bacterial

Mutation

Residues 29-313 of isoform 2

Promoter

T7 promotor

Available since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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MO70-mJP-4

Plasmid

#79598

Purpose

Transient and retroviral expression

Depositor

Yousang Gwack

Publication

Woo et al Proc Natl Acad Sci U S A. 2016 Mar 8;113

Insert

Junctophilin-4 (Jph4 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

June 29, 2016

Availability

Academic Institutions and Nonprofits only
pHygromycin (GB0211)

Plasmid

#68204

Purpose

Provides the CDS of the Hygromycin resistance gene as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Hygromycin resistance gene coding region

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

Oct. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pCool-hDDB1-BPB

Plasmid

#48639

Purpose

Expresses DDB1 BPB domain (aa 391-709) in Ecoli (ampR)

Depositor

Ning Zheng

Publication

Li et al Cell. 2006 Jan 13;124(1):105-17.

Insert

hDDB1-BPB (DDB1 Human)

Expression

Bacterial

Available since

Oct. 15, 2013

Availability

Academic Institutions and Nonprofits only
pAO139 (Apr LEU2 mrcl-AQ)

Plasmid

#41924

Depositor

Stephen Elledge

Publication

Osborn et al Genes Dev. 2003 Jul 15;17(14):1755-67.

Insert

Mediator of the Replication Checkpoint

Mutation

MRC1AQ

Available since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only
pQTEV-COPE

Plasmid

#31585

Depositor

Konrad Buessow

Publication

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

coatomer protein complex, subunit epsilon (COPE Human)

Tags

His and TEV

Expression

Bacterial

Available since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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HA-mULK1M92A

Plasmid

#31964

Depositor

Do-Hyung Kim

Publication

Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ULK1 (Ulk1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

M92A

Promoter

CMV, SP6

Available since

Oct. 31, 2011

Availability

Academic Institutions and Nonprofits only
pGEX-5X1 Flag PA1

Plasmid

#15560

Depositor

Kai Ge

Publication

Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

Insert

PA1 (PAGR1 Human)

Tags

Flag and GST

Expression

Bacterial

Available since

Aug. 16, 2007

Availability

Academic Institutions and Nonprofits only
pTMN140 - UCOE-SFFV-VPH-XTEN-dCas9-IRES2-BFP-T2A-Blast

Plasmid

#238166

Purpose

Express VPH-dCas9 and BFP-T2A-BlasticidinR in mammalian cells. Lentiviral

Depositor

Thomas Norman

Publication

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Insert

VPH-dCas9

Use

CRISPR and Lentiviral

Tags

mTagBFP2

Available since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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PT7lacO-His6-AlaRS

Plasmid

#224362

Purpose

Express AlaRS protein for his-tagged purification

Depositor

Richard Murray

Publication

Zhang et al ACS Synth Biol. 2025 May 16;14(5):1496-1

Insert

AlaRS (alaS E. coli)

Tags

His6

Expression

Bacterial

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-FAB(mito-LD)

Plasmid

#214421

Purpose

Detection of mito-lipid droplet contact site

Depositor

Chi-Lun Chang

Publication

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspAN-3xHA-V5-mito_IRES_CFAST-20nm-Halo-6xHp

Use

Lentiviral

Tags

CFAST-Halo and RspAN-3XHA-V5

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-FAB(ER-mito)

Plasmid

#214423

Purpose

Detection of ER-mito contact site

Depositor

Chi-Lun Chang

Publication

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspAN-FRB-V5-Sec61_IRES_CFAST10-20nm-Halo-mito

Use

Lentiviral

Tags

CFAST10-Halo and RspAN-FRB-V5

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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EFS-SpdCas9

Plasmid

#210703

Purpose

This plasmid express EFS promoter driven human codon optimized SpdCas9

Depositor

Isaac Hilton

Publication

Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

Insert

S. Pyogenes dCas9

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

D10A/H840A

Promoter

EF1a core promoter

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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Nsp3 Mac1 - P43 (Oxford)

Plasmid

#169210

Purpose

Expression of 6xHis tagged protein for crystallization, includes TEV protease site for tag removal

Depositor

James S Fraser

Publication

Schuller et al Sci Adv. 2021 Apr 14;7(16). pii: 7/16/ea

Insert

SARS-CoV-2 Nsp3 Mac1

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

July 28, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pG12-Rluc8

Plasmid

#170272

Purpose

Renilla reniformisluciferase (RLuc8) expression in mammlian cells. Reporter plasmid.

Depositor

Vladislav Verkhusha

Publication

Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RLuc8

Expression

Mammalian

Promoter

12xUAS with TATA-box minimal promoter

Available since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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FRB-CD28TMD-CTEVp(WT)-CS(M)-tTA

Plasmid

#137827

Purpose

MESA split-TEV protease chain with FRB Rapamycin ectodomain, CD28 transmembrane domain, C-terminal WT TEV protease, M cleavage sequence, and tTA transcription factor

Depositor

Joshua Leonard

Publication

Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FRB-CD28TMD-CTEVp(WT)-CS(M)-tTA

Tags

3x FLAG

Expression

Mammalian

Promoter

CMV

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pFlox_N-Nipbl_Neo_FKBPF36V_mScarlet

Plasmid

#163883

Purpose

Acute Depletion NIPBL-mScarlet

Depositor

Cornelis Murre

Publication

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Nipbl (Nipbl Mouse)

Expression

Mammalian

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pLX-B3omega1 (GB1694)

Plasmid

#160643

Purpose

pLX series: pBBR1-based T-DNA binary vector for plant transformation (omega1)

Depositor

Juan Antonio García, Fabio Pasin

Publication

Pasin et al ACS Synth Biol. 2017 Oct 20;6(10):1962-1

Type

Empty backbone

Use

Synthetic Biology

Available since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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