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Plasmid#121708PurposepMVP L5-L4 entry plasmid, contains eGFP-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term eGFP linked by P2A to gene of interest.DepositorInserteGFP-P2A (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET51b-Strep-SsOGT-H5-His
Plasmid#167277PurposeOverexpression of a sulfolobal equivalent of SNAP-tag in E. coliDepositorInsertSsOGT-H5
UseTagsHis x10 and Strep-tagExpressionBacterialMutationPromoterT7Available sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
KP301: pMVP (L5-L4) mCherry-P2A
Plasmid#121710PurposepMVP L5-L4 entry plasmid, contains mCherry-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term mCherry linked by P2A to gene of interest.DepositorInsertmCherry-P2A (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJet-CMV-CD5HA2-bglpA
Plasmid#96962PurposeExpression plasmid for HA-tag embedded into human CD52 with short polyADepositorInsertCMV-CD5HA2-bglpA
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
p3E-rev_U6_MCS
Plasmid#109769PurposeMultisite gateway vector for 3' expression of a guide RNA from the U6 promoter (cassette in the reverse orientation). Contains BseRI sites for insertion of gRNA sequenceDepositorInsertU6-gRNA cassette
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-rab8b
Plasmid#109658PurposeMuscle specific zebrafish expression of mKate2 fused to rab8bDepositorInsertrab8b (rab8b Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-rab1ab
Plasmid#109606PurposeMuscle specific zebrafish expression of mKate2 fused to rab1abDepositorInsertrab1ab (rab1ab Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-Rac1-DN
Plasmid#109530PurposeMuscle specific zebrafish expression of mKate2 fused to dominant negative Rac1DepositorInsertRac1 (rac1a Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationDNPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lentiviral CaMKKbeta K193A
Plasmid#33323DepositorInsertCalcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)
UseLentiviralTagsFlagExpressionMutationK194APromoterhuman PGKAvailable sinceApril 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-flex-rev-g2-2A-Venus
Plasmid#71410PurposeThis plasmid is pAAV-panpromoter-flex-rev-gamma2F77-2A-Venus. Confers zolpidem sensitivity on zolpidem-insensitive neurons.DepositorInsertsUseAAV and Cre/LoxTagsExpressionMammalianMutationPromotermouse hdc gene promoter, works as a pan neuronal …Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
actc1b-BTK-mKate2
Plasmid#109501PurposeMuscle specific zebrafish expression of mKate2 fused to the PH domain from Human Bruton tyrosine kinase (BTK). Binds PtdIns(3,4,5)P3.DepositorInsertactc1b-BTK-mKate2
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGST2-CeEP55
Plasmid#21501DepositorInsertCEP55-EABR (ESCRT and ALIX Binding Region) domain (CEP55 Human)
UseTagsGSTExpressionBacterialMutationQ230 (CAG changed to TAA), Q218 (CAA changed to T…PromoterAvailable sinceFeb. 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
HE401: pMVP (L1-R5) shRNA
Plasmid#121698PurposepMVP L1-R5 entry plasmid, contains empty H1-driven shRNA expression cassette for 4-component MultiSite Gateway Pro assemblyDepositorTypeEmpty backboneUseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIRTetR-GAL80
Plasmid#185853PurposePlugging the mechanism of tetracycline-mediated repression of GAL promoters in Saccharomyces cerevisiaeDepositorInsertgal80Arm1-PAgTEF1-KanMX4-TAgTEF1-PHAC1>TetR>TUP1>PABF1- PTEF1+[TetO]>GAL80Arm2
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSCV-miRE_shBRD9_508-SV40-GFP
Plasmid#75138PurposeRetroviral expression plasmid encoding shBRD9_508 (targeting human BRD)DepositorInsertshBRD9_508
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Topo Ptn in situ probe
Plasmid#45632DepositorInsertPtn in situ probe (Ptn Mouse)
UseIn situTagsExpressionMutationfragment contains bp# 1241-1830 of NM_008973PromoterAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-RhoAa
Plasmid#109525PurposeMuscle specific zebrafish expression of mKate2 fused to RhoAaDepositorInsertRhoAa (rhoaa Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
XW20
Plasmid#65836PurposePunc-4-QF-3'UTR-unc-54 (The endogenous SphI, KpnI and SacI sites in QF are removed, and no SL2-mcherry)DepositorInsertOR74A quinic acid utilization activator (qa-1F)
UseTagsExpressionWormMutationThe endogenous SphI, KpnI and SacI sites in QF ar…PromoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-EHD1b
Plasmid#109514PurposeMuscle specific zebrafish expression of mKate2 fused to EHD1bDepositorInsertEHD1b (ehd1b Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-FAPP1
Plasmid#109555PurposeMultisite gateway vector for 3' tagging with the PH domain from Human pleckstrin homology domain containing A3 (PLEKHA3). Binds PtdIns4PDepositorInsertthe PH domain from Human pleckstrin homology domain containing A3 (PLEKHA3).
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only