We narrowed to 15,826 results for: grna

1. CAGGS-AsCpf1-2A-GFP-U6-AAVS1-MAFB-sgRNA

   Plasmid

   #194725

   Purpose

   based on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 159281), with guide RNA array targeting both AAVS1 and MAFB

   Depositor

   Rudolf Jaenisch

   Article

   Haiting et al bioRxiv

   Insert

   AsCpf1 (MAFB, AAVS1 Acidaminococcus sp.)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D908A

   Promoter

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLV hUbC-Cas9-P2A-Puro_ST-sgRNA

   Plasmid

   #188705

   Purpose

   Lentivirus transfer plasmid encoding Cas9, puromycin resistance, and 4 sgRNAs targeting human safe targeting loci

   Depositor

   David Tobin

   Article

   Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

   Insert

   ST sgRNAs
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hUbC

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGW02 AAV-TBG-Cre-sgP53-sgRNA

   Plasmid

   #192162

   Purpose

   AAV-TBG-Cre-sgP53-sgRNA

   Depositor

   Sidi Chen

   Article

   Wang et al Cancer Discov. 2020 Dec;10(12):1912-1933

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   Available Since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLenti KO CLDN1 2xgRNA - spCas9 iRFP670 puro

   Plasmid

   #166133

   Purpose

   This plasmid allows efficient KO of the cldn1 gene, while expressing the far red fluorescent protein iRFP670 and puromycin resistance

   Depositor

   Raphael Gaudin

   Article

   Deffieu et al Hepatology. 2022 Apr 7. doi: 10.1002/hep

   Insert

   hU6-gRNA and hH1-gRNA targeting cldn1 gene
   

   Use

   CRISPR and Lentiviral

   Tags

   iRFP670

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSB700 lenti gRNA EBFP2_TtoC and hairpin extension

   Plasmid

   #167921

   Purpose

   Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSB700 lenti gRNA Blasto_TtoC and hairpin extension

   Plasmid

   #167920

   Purpose

   Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSB700 lenti gRNA EYFP_TtoC and hairpin extension

   Plasmid

   #167922

   Purpose

   Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSB700 lenti gRNA Hygro_TtoC and hairpin extension

   Plasmid

   #167923

   Purpose

   Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSB700 lenti gRNA iRFP713_TtoC and hairpin extension

   Plasmid

   #167924

   Purpose

   Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSB700 lenti gRNA Puro_TtoC and hairpin extension

    Plasmid

    #167927

    Purpose

    Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDY156: SUZ12 px330 sgRNA Cas9 plasmid

    Plasmid

    #170792

    Purpose

    This plasmid encodes Cas9 and a sgRNA that targets the SUZ12 locus; to be used with pDY153

    Depositor

    Thomas Cech

    Article

    Youmans et al Mol Cell. 2021 Feb 4;81(3):488-501.e9. d

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-3)

    Plasmid

    #159664

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available Since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-2)

    Plasmid

    #159663

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available Since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-1)

    Plasmid

    #159662

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available Since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-1)

    Plasmid

    #159656

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available Since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNTI743 dual-barcoded gRNA parent vector

    Plasmid

    #164915

    Purpose

    CEN/ARS Parent vector for constructing barcoded-gRNA CiBER-seq plasmid libraries

    Depositor

    Nicholas Ingolia

    Article

    Muller et al Science. 2020 Dec 11;370(6522). pii: 370

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDGB3 alpha1 U6-26-1gRNA-DFR 2.1 (GB1838)

    Plasmid

    #160625

    Purpose

    GB-cassette for the expression of a guide RNA targeting the DFR promoter with 2.1 Ms2 aptamer in the 3' of the scaffold

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    U6-26-1gRNA-DFR 2.1
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    U6-26

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Lenti sgRNA NGFR GFP out of frame

    Plasmid

    #155282

    Purpose

    Lentiviral plasmid for sgRNA, NGFR marker, editing detected with GFP, used with 155280

    Depositor

    Martin Carroll

    Insert

    GFP out of frame IRES NGFR
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Lenti sgRNA NGFR CFP out of frame

    Plasmid

    #155283

    Purpose

    Lentiviral plasmid for sgRNA, NGFR marker, editing detected with CFP, used with 155280

    Depositor

    Martin Carroll

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLKO.1-puro-U6-NmeCas9-TLR-MCV1-sgRNA

    Plasmid

    #117407

    Purpose

    NmeCas9 sgRNA targeting TLR2.0

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    NmeCas9 sgRNA targeting TLR 2.0
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLKO.1-puro-U6-CjeCas9-TLR-MCV1-sgRNA

    Plasmid

    #117408

    Purpose

    CjeCas9 sgRNA targeting TLR2.0

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    CjeCas9 sgRNA targeting TLR 2.0
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLKO.1-puro-U6-SauCas9-TLR-MCV1-sgRNA

    Plasmid

    #117409

    Purpose

    SauCas9 sgRNA targeting TLR2.0

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    SauCas9 sgRNA targeting TLR 2.0
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLKO.1-puro-U6-LbCas12a-TLR-MCV1-gRNA

    Plasmid

    #117410

    Purpose

    LbCas12a-gRNA targeting TLR2.0

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    LbCas12a gRNA targeting TLR 2.0
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-3

    Plasmid

    #128764

    Purpose

    Expresses DIS3L2 gRNA for CRISPRi

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    DIS3L2 sgRNA #3 (DIS3L2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available Since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-2

    Plasmid

    #128763

    Purpose

    Expresses DIS3L2 gRNA for CRISPRi

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    DIS3L2 sgRNA #2 (DIS3L2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available Since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-1

    Plasmid

    #128762

    Purpose

    Expresses DIS3L2 gRNA for CRISPRi

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    DIS3L2 sgRNA #1 (DIS3L2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available Since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pU6-sgRNA EF1Alpha-puro-T2A-BFP.PARN-2

    Plasmid

    #128760

    Purpose

    Expresses PARN gRNA for CRISPRi

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    PARN sgRNA #2 (PARN Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-3

    Plasmid

    #128758

    Purpose

    Expresses PAPD4 gRNA for CRISPRi

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    PAPD4 sgRNA #3 (TENT2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-1

    Plasmid

    #128756

    Purpose

    Expresses PAPD4 gRNA for CRISPRi

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    PAPD4 sgRNA #1 (TENT2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-2

    Plasmid

    #128757

    Purpose

    Expresses PAPD4 gRNA for CRISPRi

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    PAPD4 sgRNA #1 (TENT2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge