We narrowed to 5,566 results for: SPI

1. pET-21a_2xNLS_LbCpf1_protein_expression

   Plasmid

   #114366

   Purpose

   Protein expression plasmid for 2xNLS LbCpf1 in pET-21a backbone

   Depositor

   Scot Wolfe

   Article

   Xu et al Blood. 2019 Jan 31. pii: blood-2019-01-8

   Insert

   2xNLS_LbCpf1
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCDNA3 NQO1

   Plasmid

   #61730

   Purpose

   Expresses WT human NQO1

   Depositor

   Yosef Shaul

   Article

   Adamovich et al Mol Cell Biol. 2013 Jul;33(13):2603-13.

   Insert

   NAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)
   

   Use

   Tags

   None

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJRH056

   Plasmid

   #171637

   Purpose

   Plasmid containing a U6 promoter expressing a spyCas9 tdTom sgRNA298 and CAG promoter expressing mTagBFP2

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-tdTomato
   mTagBFP2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and U6

   Available since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. U6-3' MS2-CircRNA

   Plasmid

   #213751

   Purpose

   For circular RNA-mediated prime editor using U6-3' MS2-CircRNA in HEK293T cells

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Insert

   5' ribozyme, 5' ligation sequences, 3' MS2, 3' ligation sequences, 3' ribozyme
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. paGFP-HRas

   Plasmid

   #18693

   Depositor

   Karel Svoboda

   Article

   Harvey et al Science. 2008 Jul 4. 321(5885):136-40.

   Insert

   H-Ras (HRAS Human)
   

   Use

   Tags

   photoactivatable GFP

   Expression

   Mammalian

   Mutation

   A206K mutation in paGFP

   Promoter

   Available since

   July 2, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEYFP-Pds1(434)

   Plasmid

   #39847

   Depositor

   Jonathon Pines

   Article

   Hagting et al J Cell Biol. 2002 Jun 24;157(7):1125-37.

   Insert

   Pds1 (PTTG1 Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 31, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJM160

   Plasmid

   #134169

   Purpose

   Bacterial expression of RB3 stathmin-like domain (SLD)

   Depositor

   Iain Cheeseman

   Article

   Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

   Insert

   RB3-SLD (STMN4 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   residues 49-189

   Promoter

   Available since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-dCpf1-eBE-YE

   Plasmid

   #107689

   Purpose

   Expresses dLbCpf1-eBE-YE in mammalian cells

   Depositor

   Jia Chen

   Article

   Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   dLbCpf1-eBE-YE
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   APOBEC1(W90Y/R126E), dLbCpf1(D832A/E925A/D1148A)

   Promoter

   CMV

   Available since

   June 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAT024

   Plasmid

   #171636

   Purpose

   Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting BFP and CAG promoter expressing mTagBFP2

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-BFP
   mTagBFP2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and U6

   Available since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHis-zfCSF3b

    Plasmid

    #65613

    Purpose

    express soluble His-tagged zebrafish CSF3b/Gcsfb in E. coli

    Depositor

    Petr Bartunek

    Article

    Stachura et al Blood. 2013 Dec 5;122(24):3918-28. doi:

    Insert

    Csf3b (csf3b Zebrafish)
    

    Use

    Tags

    (His)6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pBLO2 casX

    Plasmid

    #123122

    Purpose

    E. coli expression vector for CasX sgRNA

    Depositor

    Benjamin Oakes, Jennifer Doudna

    Article

    Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

    Insert

    CasX guide RNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGBW-m4046799

    Plasmid

    #145577

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pAd-Track SIRT1 H355A

    Plasmid

    #8441

    Depositor

    Pere Puigserver

    Article

    Rodgers et al Nature 2005 Mar 3;434(7029):113-8.

    Insert

    SIRT1 (Sirt1 Mouse)
    

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Histidine 355 mutated to Alanine

    Promoter

    Available since

    Dec. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pLacI-LAP-BUB1

    Plasmid

    #115905

    Purpose

    Used to express BUB1-WT fused to LacI for recruitment of BUB1 to LacO spots.

    Depositor

    Geert Kops

    Article

    Vleugel et al J Cell Sci. 2015 Aug 15;128(16):2975-82.

    Insert

    LacI-BUB1 (BUB1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCRII-Topo Plexin D1 in situ probe

    Plasmid

    #45621

    Depositor

    Jeffrey Macklis

    Article

    Molyneaux et al Neuron. 2005 Sep 15;47(6):817-31.

    Insert

    Plexin D1 in situ probe (Plxnd1 Mouse)
    

    Use

    In situ

    Tags

    Expression

    Mutation

    fragment contains bp# 5751-6406 of NM_026376.3

    Promoter

    Available since

    July 9, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pLV-75-2

    Plasmid

    #73036

    Purpose

    Express an NT-3-BDNF hybrid neurotrophic factor, with reduced affinity to p75NTR, in mammalian cells

    Depositor

    Pantelis Tsoulfas

    Article

    Enomoto et al Exp Neurol. 2013 Oct;248:170-82. doi: 10

    Insert

    p75-2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Mature Human NT-3 gene exchanged Aspartic 15 to a…

    Promoter

    Available since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pYPQ230-RV

    Plasmid

    #188544

    Purpose

    Gateway entry clone (attL1 & attR5) for LbCas12a-RV mediated genome editing

    Depositor

    Yiping Qi

    Article

    Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:

    Insert

    LbCas12a-RV
    

    Use

    CRISPR; Gateway compatible lbcas12a-rv entry clone

    Tags

    NLS

    Expression

    Plant

    Mutation

    G146R, R182V

    Promoter

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CoV-NSP3-c001

    Plasmid

    #173082

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    rep
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    E1000-K1173, codon optimized

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAGT8162 (AATG_dttLbCas12a-i_TTCG)

    Plasmid

    #192350

    Purpose

    L0 MoClo-compatible GoldenGate-module of dttLbCas12a-i for in planta expression

    Depositor

    Tom Schreiber

    Article

    Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

    Insert

    AATG_dttLbCas12a-i_TTCG
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D156R; E925Q

    Promoter

    Available since

    Nov. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLaNGo11-1

    Plasmid

    #126053

    Purpose

    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Inserts

    chloroplastic FNR transit peptide
    ccdB cassette (BsaI)
    GFP11
    

    Use

    Binary

    Tags

    GFP1-10

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge