We narrowed to 11,278 results for: VARS

1. pXJ-Myc-GATA3

   Plasmid

   #232646

   Purpose

   transient expresses GATA3 in mammalian cells

   Depositor

   Chen-Yang Shen

   Article

   Chou et al NPJ Genom Med. 2022 Jan 19;7(1):4. doi:

   Insert

   GATA3 (GATA3 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFA6a-FLAG-APEX2-NES-K.l.LEU2

   Plasmid

   #229229

   Purpose

   Template plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeast

   Depositor

   Fulvio Reggiori

   Article

   Filali-Mouncef et al Autophagy. 2024 Oct;20(10):2323-2337. do

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFA6a-FLAG-APEX2-NES-NatMX6

   Plasmid

   #229231

   Purpose

   Template plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeast

   Depositor

   Fulvio Reggiori

   Article

   Filali-Mouncef et al Autophagy. 2024 Oct;20(10):2323-2337. do

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFA6a-FLAG-APEX2-NES-TRP1

   Plasmid

   #229228

   Purpose

   Template plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeast

   Depositor

   Fulvio Reggiori

   Article

   Filali-Mouncef et al Autophagy. 2024 Oct;20(10):2323-2337. do

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS puro human RLIM N581K

   Plasmid

   #232242

   Purpose

   Mammalian expression of Human RLIM N581K

   Depositor

   Francisco Bustos

   Article

   Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

   Insert

   Human RLIM N581K (RLIM Human)
   

   Expression

   Mammalian

   Mutation

   N581K

   Available Since

   Feb. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLANT-2b

   Plasmid

   #231487

   Purpose

   Expresses His6-SUMO^NIT-FraB where "NIT" stands for "no internal translation", reflecting the mutation of the two Shine-Dalgarno-like sequences within the SUMO tag.

   Depositor

   Venkat Gopalan

   Inserts

   SUMO^NIT (SMT3 Budding Yeast)
   FraB
   

   Tags

   His6 tag and His6-SUMO^NIT

   Expression

   Bacterial

   Mutation

   Deleted amino acids 1-6 (MEPEES). and M83L, E84Q,…

   Promoter

   T7 promoter

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX6P1 human RLIM N581K

   Plasmid

   #232241

   Purpose

   Protein expression of GST-human RLIM N581K

   Depositor

   Francisco Bustos

   Article

   Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

   Insert

   GST-human RLIM N581K (RLIM Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   N581K

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-shRNA2-zsGreen-PGK-puro_HSALNG0104472-163

   Plasmid

   #197902

   Purpose

   For the stable knockdown experiments using shRNA, a complementary sequence (HSALNG0104472-163) targeting the HSALNG0104472 transcript was inserted.

   Depositor

   Bo Wang

   Article

   Lu et al Commun Biol. 2023 Feb 17;6(1):189. doi:

   Insert

   HSALNG0104472-163 (HSALNG0104472-scramble-sequence)
   

   Use

   Lentiviral

   Tags

   zsGreen1

   Expression

   Mammalian

   Mutation

   15q11.2 deletion

   Available Since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-Cas9-FCPF

   Plasmid

   #220132

   Purpose

   Expresses Cas9-FCPF in E.coli

   Depositor

   Xinlai Cheng

   Article

   Altinbay et al Nucleic Acids Res. 2024 Sep 24:gkae798.

   Insert

   Cas9-FCPF (cas9 S. pyogenes)
   

   Tags

   His and NLS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. dCas9-FCPF

    Plasmid

    #220131

    Purpose

    Expresses dCas9-FCPF in mammalian cells

    Depositor

    Xinlai Cheng

    Article

    Altinbay et al Nucleic Acids Res. 2024 Sep 24:gkae798.

    Insert

    dCas9-FCPF (cas9 S. pyogenes, Synthetic)
    

    Tags

    Flag and HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-dCas9-FCPF

    Plasmid

    #220133

    Purpose

    Expresses dCas9-FCPF in E.coli

    Depositor

    Xinlai Cheng

    Article

    Altinbay et al Nucleic Acids Res. 2024 Sep 24:gkae798.

    Insert

    dCas9-FCPF (cas9 S. pyogenes, Synthetic)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    FCPF sequence inserted on C-terminal

    Promoter

    T7

    Available Since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDUP2

    Plasmid

    #223517

    Purpose

    cogfp copy 1 under control of Ptet, cogfp copy 2 under control of Ptac

    Depositor

    Yolanda Schaerli

    Article

    Ljiljana et al eLife13:RP97216

    Inserts

    CoGFP
    CoGFP (second copy)
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    anti-dimerization mutations S129G, 22 C154S, D156…

    Promoter

    Ptac, Ptac and Ptet, and Ptet

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFastBac1-NpSxph

    Plasmid

    #193097

    Purpose

    Protein expression in insect cells

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Nanorana parkeri saxiphilin
    

    Tags

    3C-eGFP-His10

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFastBac1-NpSxphI559Y

    Plasmid

    #193099

    Purpose

    Protein expression in insect cells

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Nanorana parkeri saxiphilin I559Y
    

    Tags

    3C-eGFP-His10

    Expression

    Insect

    Mutation

    I559Y

    Promoter

    polyhedrin

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFastBac1-OsSxph

    Plasmid

    #193100

    Purpose

    Protein expression in insect cells

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Oophaga sylvatica saxiphilin
    

    Tags

    3C-eGFP-His10

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. RcSxph E540A

    Plasmid

    #194019

    Purpose

    Protein expression and characterization

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Rana catesbeiana Saxiphilin
    

    Tags

    His10 and mEGFP,

    Expression

    Insect

    Mutation

    E540A

    Promoter

    Polyhedrin

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. RcSxph E540D

    Plasmid

    #194020

    Purpose

    Protein expression and characterization

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Rana catesbeiana Saxiphilin
    

    Tags

    His10 and mEGFP,

    Expression

    Insect

    Mutation

    E540D

    Promoter

    Polyhedrin

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. RcSxph E540Q

    Plasmid

    #194021

    Purpose

    Protein expression and characterization

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Rana catesbeiana Saxiphilin
    

    Tags

    His10 and mEGFP,

    Expression

    Insect

    Mutation

    E540Q

    Promoter

    Polyhedrin

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. RcSxph Y558A

    Plasmid

    #194022

    Purpose

    Protein expression and characterization

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Rana catesbeiana Saxiphilin
    

    Tags

    His10 and mEGFP,

    Expression

    Insect

    Mutation

    Y558A

    Promoter

    Polyhedrin

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. RcSxph Y558F

    Plasmid

    #194023

    Purpose

    Protein expression and characterization

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Rana catesbeiana Saxiphilin
    

    Tags

    His10 and mEGFP,

    Expression

    Insect

    Mutation

    Y558F

    Promoter

    Polyhedrin

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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