We narrowed to 17,568 results for: bon;

1. Str-KDEL_SBP-EGFP-PLAP

   Plasmid

   #222308

   Purpose

   Synchronize the trafficking of PLAP from the ER.

   Depositor

   Franck Perez

   Article

   Fourriere et al J Cell Biol. 2019 Jul 1;218(7):2215-2231

   Insert

   Streptavidin-KDEL and PLAP fused to SBP-EGFP (ALPP Human)
   

   Expression

   Mammalian

   Mutation

   signal peptide removed

   Promoter

   CMV

   Available Since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. gRNA giantin/GOLGB1

   Plasmid

   #222315

   Purpose

   CRISPR/Cas9 close to the ATG of giantin/GOLGB1 gene.

   Depositor

   Franck Perez

   Article

   Fourriere et al J Cell Sci. 2016 Sep 1;129(17):3238-50.

   Insert

   gRNA targeting GOLB1 (GOLGB1 Human)
   

   Use

   CRISPR

   Available Since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTRE3G-WHG cGAS-HA

   Plasmid

   #186867

   Purpose

   Doxycycline-dependent expression of white-handed gibbon cGAS gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

   Insert

   White-handed gibbon cGAS with C-terminal HA tag
   

   Use

   Retroviral

   Tags

   HA

   Promoter

   TRE3G

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPPI4-Strep-SNAP-PD-L2-His10

   Plasmid

   #223578

   Purpose

   Protein purification of human PD-L2 with SNAP and 10xHis tags in mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

   Insert

   PD-L2 (PDCD1LG2 Human)
   

   Tags

   His10 and Twin-Strep, SNAP

   Expression

   Mammalian

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBK2043-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9

   Plasmid

   #223163

   Purpose

   2nd gen. AAV backbone for dSaCas9 (endonuclease dead Cas9 from Staphylococcus aureus) and Sa-gRNA Scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 204-310 (MECP2)

   Available Since

   Aug. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-VcnTS-E1015A

   Plasmid

   #213415

   Purpose

   Vinculin tension sensor (VcnTS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1015A).

   Depositor

   Brenton Hoffman

   Article

   Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

   Insert

   VcnTS-E1015A (VCL Chicken, Synthetic)
   

   Expression

   Mammalian

   Mutation

   Mutated vinculin glutamic acid 1015 to alanine (E…

   Promoter

   CMV

   Available Since

   Feb. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-VcnTS-E1021A

   Plasmid

   #213416

   Purpose

   Vinculin tension sensor (VcnTS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1021A).

   Depositor

   Brenton Hoffman

   Article

   Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

   Insert

   VcnTS-E1021A (VCL Chicken, Synthetic)
   

   Expression

   Mammalian

   Mutation

   Mutated vinculin glutamic acid 1021 to alanine (E…

   Promoter

   CMV

   Available Since

   Feb. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-VcnCS-E1015A

   Plasmid

   #213412

   Purpose

   Vinculin conformation sensor (VcnCS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1015A).

   Depositor

   Brenton Hoffman

   Article

   Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

   Insert

   VcnCS-E1015A (VCL Chicken, Synthetic)
   

   Expression

   Mammalian

   Mutation

   Mutated vinculin glutamic acid 1015 to alanine (E…

   Promoter

   CMV

   Available Since

   Feb. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-VcnCS-E1021A

   Plasmid

   #213413

   Purpose

   Vinculin conformation sensor (VcnCS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1021A).

   Depositor

   Brenton Hoffman

   Article

   Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

   Insert

   VcnCS-E1021A (VCL Chicken, Synthetic)
   

   Expression

   Mammalian

   Mutation

   Mutated vinculin glutamic acid 1021 to alanine (E…

   Promoter

   CMV

   Available Since

   Feb. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-DIO-basic

    Plasmid

    #196882

    Purpose

    Used for preparation of double-floxed inverse ORF vectors

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Type

    Empty backbone

    Use

    Cre/Lox; Dre/rox

    Expression

    Mammalian

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGA-red-mini

    Plasmid

    #196338

    Purpose

    Easy-MISE toolkit Level 1 destination plasmid for cassette cloning with BsaI-based Golden Gate Assembly; allowing fast postitive clones test with mRFP1 fluorescent protein-based red/white screening

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Type

    Empty backbone

    Use

    Synthetic Biology; Golden gate assembly backbone

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGA-red-maxi

    Plasmid

    #196337

    Purpose

    Easy-MISE toolkit Level 1 destination plasmid for cassette cloning with BsaI-based Golden Gate Assembly; allowing fast postitive clones test with mRFP1 fluorescent protein-based red/white screening

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Type

    Empty backbone

    Use

    Synthetic Biology; Golden gate assembly backbone

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGA-blue

    Plasmid

    #196336

    Purpose

    Easy-MISE toolkit Level 0 destination plasmid for parts cloning with Esp3I-based Golden Gate Assembly; allowing fast postitive clones test with AmilCP chromoprotein-based blue/white screening

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Type

    Empty backbone

    Use

    Synthetic Biology; Golden gate assembly backbone

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRA1SEGFPTcIfm

    Plasmid

    #193777

    Purpose

    Cassette 1: Expresses EGFP under the control of PR promoter, Cassette 2: Expresses frame-shifted CI under the control of PLTetO-1 promoter, pUC origin of replication, Ampicillin selection

    Depositor

    Sangram Bagh

    Article

    Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

    Insert

    EGFP and frame-shifted CI in opposite orientation, controlled by separate promoters and terminators
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PR promoter and PLTetO-1

    Available Since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTA1cI-T3

    Plasmid

    #193789

    Purpose

    Expresses truncated version of lambda CI named T3 under the control of PLTetO-1 promoter, pUC origin of replication, Ampicillin selection

    Depositor

    Sangram Bagh

    Article

    Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

    Insert

    Truncated version of Lambda CI, named T3, where the first two codons are deleted
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PLTetO-1

    Available Since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGlomyc-Ovol1

    Plasmid

    #172871

    Purpose

    Mammalian expression vector with myc-tagged mouse Ovol1 coding sequence

    Depositor

    Renee van Amerongen

    Article

    Mourao et al bioRxiv 2021.06.22.449461

    Insert

    Ovol1 (Ovol1 Mouse)
    

    Tags

    myc

    Expression

    Mammalian

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMP71-mGFP-llo190-mc38minor

    Plasmid

    #174604

    Purpose

    expresses palmitoylated GFP with a C terminal fusion of the LLO190 and minigenes of 3 neoantigens from the MC38 tumor in genes Aatf, irgq and cpne1

    Depositor

    Stanley Riddell

    Article

    Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

    Insert

    palmitoyl-GFP C-term fusion LLO190 and minigenes of 3 neoantigens from MC38 tumor in genes Aatf irgq cpne1
    

    Expression

    Mammalian

    Available Since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGMC00008

    Plasmid

    #166724

    Purpose

    sgRNA cloning backbone

    Depositor

    Raj Chari

    Article

    Choi et al Nat Commun. 2020 Jun 1;11(1):2718. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lenti-XYLT2-sgRNA

    Plasmid

    #154862

    Purpose

    Lentiviral expression of Cas9 and sgRNA targeting XYLT2

    Depositor

    Yihong Ye

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

    Insert

    XYLT2 sgRNA (XYLT2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET14b-dabAB2 D353A

    Plasmid

    #133016

    Purpose

    pET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a D353A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Inserts

    dabB2
    dabA2
    

    Tags

    6xHis, StrepII tag, and sfGFP

    Expression

    Bacterial

    Mutation

    D353A

    Promoter

    t7

    Available Since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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