We narrowed to 4,353 results for: erf

1. pSLQ5080_pHR_PGK_sfGFP_CoV-F2

   Plasmid

   #155304

   Purpose

   SARS-CoV-2 fluorescent reporter 2

   Depositor

   Stanley Qi

   Article

   Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

   Insert

   Superfolder GFP fused with SARS-CoV-2 fluorescent reporter 2 (N, ORF1ab Synthetic)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available Since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGW011b Dual AAV vector pAAV-EFS-dCAS9-spA

   Plasmid

   #192160

   Purpose

   Dual AAV vector AAV-dCas9

   Depositor

   Sidi Chen

   Article

   Wang et al Nat Immunol. 2019 Nov;20(11):1494-1505.

   Insert

   Dead Cas9
   

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   Available Since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Cx43K258stop-msfGFP

   Plasmid

   #69023

   Purpose

   Expresses rat Cx43 (Gja1 CDS) truncated at amino acid 258 and tagged with msfGFP on the C-terminus. CMV promoter.

   Depositor

   David Spray

   Article

   Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

   Insert

   Cx43 (Gja1 Rat)
   

   Use

   Tags

   V206K monomerized super folder GFP

   Expression

   Mammalian

   Mutation

   delete codons 258-381 and fuse in frame to monome…

   Promoter

   CMV

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNSSCP

   Plasmid

   #183188

   Purpose

   PACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; DivIVA-sfGFP expressed under T

   Depositor

   Hsiao-Chun Huang

   Article

   Lin et al Nat Commun. 2021 Feb 9;12(1):888. doi: 1

   Inserts

   DivIVA and superfolder GFP fusion protein
   SpmX450 and T7 181+ fusion protein
   T7 RNAP 1-179 variant and SpmX450 fusion protein
   mRFP1 and popZ fusion protein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330-Flag-dSpCas9-HF1

   Plasmid

   #92115

   Purpose

   Expression plasmid for human codon-optimized dead/inactive high-fidelity SpCas9-HF1 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive SpCas9-HF1 with FLAG tag
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   D10A, N497A, R661A, Q695A, H840A, Q926A

   Promoter

   Cbh

   Available Since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. LIC-Z

   Plasmid

   #153543

   Purpose

   perform light induced optogenetic clustering of TCR ζ-Chain as described in the associated publication

   Depositor

   Katharina Gaus

   Article

   Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:

   Insert

   human TCR ζ-Chain (CD247 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   CMV

   Available Since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. DF-UAS-sspa-GCaMP6f

   Plasmid

   #67569

   Purpose

   Zebrafish expression of a short photoactivatable fluorescent calcium reporter

   Depositor

   Ehud Isacoff

   Article

   Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

   Insert

   sspa-GCaMP6f
   

   Use

   Zebrafish transgenesis (see :a spinal opsin contr…

   Tags

   Expression

   Mutation

   K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

   Promoter

   Available Since

   Aug. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pST_APOE_HUMAN_E4_47-300 (pBS0847)

   Plasmid

   #185279

   Purpose

   For the mammalian expression of the human protein APOE_HUMAN_E4_47-300. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   APOE_HUMAN_E4_47-300
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330-Flag-dHeFSpCas9

   Plasmid

   #92116

   Purpose

   Expression plasmid for human codon-optimized dead/inactive increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive HeFSpCas9 with FLAG tag
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…

   Promoter

   Cbh

   Available Since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDRF'- AmTryoshka1;3-LS-F138I-T78H

    Plasmid

    #100963

    Purpose

    Yeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    AmTryoshka1;3-LS-F138I-T78H (AMT1;3 Mustard Weed)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    F138I, T78H

    Promoter

    PMA

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCCL-PGK-SPdCas9-BFP-EGFP

    Plasmid

    #66821

    Purpose

    dCas9 fused to BFP and EGFP

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-BFP-EGFP
    

    Use

    Tags

    dSpCas9-BFP-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pST_MAHS_RAMVA_81-236-FLAG (pBS0297)

    Plasmid

    #185328

    Purpose

    For the mammalian expression of the tardigrade protein MAHS_RAMVA_81-236-FLAG. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    MAHS_RAMVA_81-236-FLAG
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV_SEPT9_i5-msfGFP

    Plasmid

    #180327

    Purpose

    mammalian expression of human SEPT9_i5 fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

    Insert

    SEPTIN9_v7 (SEPTIN9 Human)
    

    Use

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. DF-UAS-spa-GCaMP6f

    Plasmid

    #67568

    Purpose

    Zebrafish expression of a photoactivatable fluorescent calcium reporter

    Depositor

    Ehud Isacoff

    Article

    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

    Insert

    spa-GCaMP6f
    

    Use

    Zebrafish transgenesis (see :a spinal opsin contr…

    Tags

    HIS-tag

    Expression

    Mutation

    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

    Promoter

    Available Since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pEF1a-CRY2PHR-SID4X

    Plasmid

    #63663

    Purpose

    AAV expression of CRYS2PHR-SID4X

    Depositor

    Dong Kong

    Article

    Chen et al Theranostics. 2018 Aug 7;8(16):4447-4461

    Insert

    SID4X-CYR2PHR
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available Since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDRF'- AmTryoshka1;3-LS-F138I

    Plasmid

    #100922

    Purpose

    Yeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    AmTryoshka1;3-LS-F138I (AMT1;3 Mustard Weed)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    F138I

    Promoter

    PMA

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDRF'- AmTryoshka1;3-LS-L255I

    Plasmid

    #100964

    Purpose

    Yeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    AmTryoshka1;3-LS-L255I (AMT1;3 Mustard Weed)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    L255I

    Promoter

    PMA

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDRF'- AmTryoshka1;3-GS-F138I

    Plasmid

    #100965

    Purpose

    Yeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    AmTryoshka1;3-GS-F138I (AMT1;3 Mustard Weed)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    F138I

    Promoter

    PMA

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

19. pDRF'- AmTryoshka1;3-GS-L255I

    Plasmid

    #100966

    Purpose

    Yeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    AmTryoshka1;3-GS-L255I (AMT1;3 Mustard Weed)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    L255I

    Promoter

    PMA

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUC19-B1UZL4

    Plasmid

    #103085

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    B1UZL4(Cas9 coding gene from Clostridium perfringens D str. JGS1721)
    

    Use

    CRISPR; Cloning vector

    Tags

    Expression

    Mutation

    human codon-optimized

    Promoter

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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