We narrowed to 4,475 results for: firefly luciferase
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Plasmid#192406PurposeTo be a control plasmid that has no Rluc repression (replaced by turboGFP) and should reflect true off state of the circuit.DepositorInsertAct2::Turbo GFP
UseSynthetic BiologyTagsN7ExpressionPlantAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP690_L2_1IF1_FRT-OCS_Rluc_FlpF1_C1-NLS
Plasmid#192413PurposeTo act as a 1-input state of the split-AND gate.DepositorInsertAct2::FRT-OCS-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP737_L2pV1_1I_Act2_FRT_OCS_Act2_Flp_lacZ
Plasmid#192380PurposeTo test if the Act2 promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.DepositorInsertAct2::FRT-OCS-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N3-FLuc-K21-Rluc_AH
Plasmid#148847PurposeMammalian Expression of K21-RlucDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertK21-Rluc
ExpressionMammalianAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNOC_dCas9-KRAB_sgRNA Td2
Plasmid#176264PurposepNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged to the KRAB domain and Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdead spCas9
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsKruppel associated box domain and luciferaseMutationH840A and D10A mutations on spCas9 to inactivate …Available sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNOC_hfnCas12a_NR1_NR2_LPT1
Plasmid#176253PurposepNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and CRISPR array with spacers 1 and 2 targeting the Nitrate reductase gene and one spacer targeting the LPAAT geneDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthumanized fnCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNOC_hfnCas12a-Nlux_crRNA NR1
Plasmid#176244PurposepNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthumanized fnCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferasePromoterRibosomal subunitAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNOC_dfnCas12a_crRNA NC
Plasmid#176256PurposepNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged with Nlux and spacer sequence is replaced by the type IIS restriction site for endonuclease BaeI that can beDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthumanized fnCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseMutationD917A and E1006A mutations to inactivate the endo…Available sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pG20_LUC2_Hyg
Plasmid#159707PurposepGREEN-based cloning construct for expression in plantaDepositorTypeEmpty backboneTagsluciferaseExpressionPlantAvailable sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
Antares-SEPLuc
Plasmid#141086Purposeprecursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosisDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAntares-T2A-puromycin-EMCV IRES-SEPLuc
ExpressionMammalianMutationDeletion of amino acids 66 to 91 on the Luciferas…PromoterCMVAvailable sinceJune 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pC-KIT2
Plasmid#118984PurposeMutational dissection of the G rich region of the c-kit promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertc-kit proto-oncogene {promoter} (KIT Human)
ExpressionMammalianMutationmutK2-SP-K1Available sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC-KIT4
Plasmid#118986PurposeMutational dissection of the G rich region of the c-kit promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertc-kit proto-oncogene {promoter} (KIT Human)
ExpressionMammalianMutationK2-SP-mutK1Available sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC-KIT5
Plasmid#118987PurposeMutational dissection of the G rich region of the c-kit promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertc-kit proto-oncogene {promoter} (KIT Human)
ExpressionMammalianMutationmutK2-mutSP-K1Available sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC-KIT6
Plasmid#118988PurposeMutational dissection of the G rich region of the c-kit promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertc-kit proto-oncogene {promoter} (KIT Human)
ExpressionMammalianMutationmutK2-SP-mutK1Available sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti PA-NL
Plasmid#113453PurposeLentiviral ProteinA-NanoLuc hybrid fusion control expression vector (for BRET and LuC normalization)DepositorInsertProteinA-NanoLuc
UseLentiviral and LuciferaseExpressionMammalianPromoterhUbCAvailable sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMIR-REPORT-MYC CDS_WT
Plasmid#108924PurposeMYC mRNA CDS region with wild type m6A motifs was inserted into pMIR-REPORT vectorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMYC (MYC Human)
ExpressionMammalianPromoterCMVAvailable sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
hSTARR-seq_SCP1 vector_blocking 1
Plasmid#99316PurposeVector to measure enhancer activity of candidates from a DNA library by STARR-seq.DepositorTypeEmpty backboneUseHstarr-seq screening vectorExpressionMammalianAvailable sinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCNL-N1_vinculin
Plasmid#65691PurposeExpresses Vinculin-CNL in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertvinculin (Vcl Mouse)
UseLuciferaseTagsCNLExpressionMammalianPromoterCMVAvailable sinceJan. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pONL-N1_LAMP1
Plasmid#65686PurposeExpresses LAMP1-ONL in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLAMP1
UseLuciferaseTagsONLExpressionMammalianPromoterCMVAvailable sinceJan. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pONL-N1_zyxin
Plasmid#65683PurposeExpresses Zyxin-ONL in mammalian cellsDepositorAvailable sinceJuly 20, 2015AvailabilityAcademic Institutions and Nonprofits only