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pMC8b-XI2up

Plasmid

#176168

Purpose

yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XI-2

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

5' homology for integration site XI-2

Use

Synthetic Biology

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC7-XI2dw

Plasmid

#176169

Purpose

yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XI-2

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

3' homology for integration site XI-2

Use

Synthetic Biology

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC8b-XII2up

Plasmid

#176174

Purpose

yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XII-2

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

5' homology for integration site XII-2

Use

Synthetic Biology

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC7-XII2dw

Plasmid

#176175

Purpose

yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XII-2

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

3' homology for integration site XII-2

Use

Synthetic Biology

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC8b-X4up

Plasmid

#176164

Purpose

yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site X-4

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

5' homology for integration site X-4

Use

Synthetic Biology

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC7-X4dw

Plasmid

#176165

Purpose

yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site X-4

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

3' homology for integration site X-4

Use

Synthetic Biology

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pEDJ-87

Plasmid

#163085

Purpose

MarkerFree plasmid for integration of pADH1-MCP-VPR into site XI-5

Depositor

Michael Jensen

Article

Jensen et al Microb Cell Fact. 2017 Mar 15;16(1):46.

Insert

VPR

Tags

MCP

Expression

Yeast

Promoter

ADH1

Available Since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pDB4953

Plasmid

#174020

Purpose

pDUAL plasmid expressing GFP-Atg6 from 41nmt1 promoter

Depositor

Li-Lin Du

Article

Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

Insert

Atg6

Tags

GFP, Atg6

Expression

Yeast

Promoter

41nmt1

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pDB4658

Plasmid

#174017

Purpose

pDUAL plasmid expressing GFP-Atg8 from 41nmt1 promoter

Depositor

Li-Lin Du

Article

Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

Insert

Atg8

Tags

GFP, Atg8

Expression

Yeast

Promoter

41nmt1

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pDB4958

Plasmid

#174014

Purpose

pHIS3H plasmid expressing CFP-Atg6 from 41nmt1 promoter

Depositor

Li-Lin Du

Article

Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

Insert

Atg6

Tags

CFP, Atg6

Expression

Yeast

Promoter

41nmt1

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pDB4924

Plasmid

#174012

Purpose

pDUAL plasmid expressing Pil1-mCherry from 41nmt1 promoter

Depositor

Li-Lin Du

Article

Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

Insert

Pil1-mCherry

Tags

Pil1, mCherry

Expression

Yeast

Promoter

41nmt1 and 41nmt1p

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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Cyterm-mAvicFP1 pDRF1-GW

Plasmid

#168092

Purpose

Expresses Cyterm-mAvicFP1 to check for monomericity.

Depositor

Bas Teusink

Article

Botman et al ACS Synth Biol. 2026 Apr 1. doi: 10.1021

Insert

mAvicFP1

Expression

Yeast

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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Cyterm-ystagRFP pDRF1-GW

Plasmid

#168061

Purpose

Expresses Cyterm-ystagRFP to check for monomericity.

Depositor

Bas Teusink

Article

Botman et al ACS Synth Biol. 2026 Apr 1. doi: 10.1021

Insert

Cyterm-ystagRFP

Expression

Yeast

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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Cyterm-AausFP1 pDRF1-GW

Plasmid

#168062

Purpose

Expresses Cyterm-AausFP1 to check for monomericity.

Depositor

Bas Teusink

Article

Botman et al ACS Synth Biol. 2026 Apr 1. doi: 10.1021

Insert

Cyterm-AausFP1

Expression

Yeast

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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Dcp2 ΔH1 Δ5H

Plasmid

#163582

Purpose

express Dcp2 ΔH1 Δ5H at endogenous level in S.cerevisiae

Depositor

Michael Rosen

Article

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

L255A; mutate 440-450, 489-500, 701-708, 827-831,…

Promoter

DCP2 promoter

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pNTI751 pPGK1-GCN4(UTR)-ZEM(TF)

Plasmid

#164916

Purpose

Expresses the ZEM transcription factor under the translational control of the yeast GCN4 5'UTR

Depositor

Nicholas Ingolia

Article

Muller et al Science. 2020 Dec 11;370(6522). pii: 370

Insert

GCN4(UTR)-ZEM(TF)

Use

Synthetic Biology

Expression

Yeast

Promoter

PGK1 (yeast)

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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p415 Glo3 S389,398 D

Plasmid

#129486

Purpose

Expression in S. cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3 S389,398 D (GLO3 Budding Yeast)

Expression

Yeast

Mutation

Mutation of serines 389 and 398 to Asp

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p415 Met25 Glo3 ΔGRM-ΔC

Plasmid

#129487

Purpose

Expression in S. cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3 ΔGRM ΔC (GLO3 Budding Yeast)

Expression

Yeast

Mutation

Δ376-493 (deletion of Glo3 motif & C-terminal…

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p415 Glo3 R59K ΔC

Plasmid

#112653

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3 R59K ΔC

Expression

Yeast

Mutation

R59K, Δ431-493 (deletion of C-terminal amphipathi…

Promoter

Met25

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p415 Glo3 R59K ΔGRM-ΔC

Plasmid

#112654

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3 R59K ΔGRM ΔC

Expression

Yeast

Mutation

R59K, Δ376-493 (deletion of Glo3 motif & C-te…

Promoter

Met25

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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