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Plasmid#239343PurposeExpresses the I3-01 nanocage subunit N-terminally tagged with mScarlet3-H in mammalian cells. Assembles into nanocages tagged with 60 FPs.DepositorInsertI3-01
UseTagsmScarlet3-H (a.k.a. mYongHong)ExpressionMammalianMutationK129APromoterCMVAvailable sinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pmStayGold(E138D)_I3
Plasmid#239338PurposeExpresses the I3-01 nanocage subunit N-terminally tagged with mStayGold(E138D) in mammalian cells. Assembles into nanocages tagged with 60 FPs.DepositorInsertI3-01
UseTagsmStayGold(E138D)ExpressionMammalianMutationK129APromoterCMVAvailable sinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBOBI-hPLD4-flag
Plasmid#222641Purposelentiviral vector for full length human PLD4 expression (with flag tag)DepositorInsertPLD4 (PLD4 Human)
UseLentiviralTagsflagExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
LLhKdgR-pHG165a 2mut
Plasmid#220270PurposeConstitutive expression of a chimeric LacI:KdgR transcription repressorDepositorInsertLLhKdgR
UseTagsExpressionBacterialMutationV52A/Q55VPromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
LLhRafR-pHG165a G62A
Plasmid#220273PurposeConstitutive expression of a chimeric LacI:RafR transcription repressorDepositorInsertLLhRafR
UseTagsExpressionBacterialMutationG62APromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
LLhRafR-pHG165a 3mut
Plasmid#220274PurposeConstitutive expression of a chimeric LacI:RafR transcription repressorDepositorInsertLLhRafR
UseTagsExpressionBacterialMutationV52N/Q55L/Q62HPromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
LLhSalR-pHG165a 3mut
Plasmid#220276PurposeConstitutive expression of a chimeric LacI:SalR transcription repressorDepositorInsertLLhSalR
UseTagsExpressionBacterialMutationV52L/Q55G/Y62RPromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
LLhScrR-pHG165a 3mut
Plasmid#220278PurposeConstitutive expression of a chimeric LacI:ScrR transcription repressorDepositorInsertLLhScrR
UseTagsExpressionBacterialMutationV52I/Q62S/G120SPromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
EP003: CAG::GEM7-HaloTag
Plasmid#209303PurposeExpression plasmid for GEM7 (encapsulin from R. rubrum) with C-terminal HaloTag suitable for expression in mice via in utero electroporationDepositorInsertGEM7
UseAAVTagsHaloTagExpressionMutationPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EP004: pAAV TRE::GEM7-HaloTag
Plasmid#209304PurposeDoxycycline-gated expression plasmid (TetOFF) for GEM7 (encapsulin from R. rubrum) with C-terminal HaloTag suitable for expression in miceDepositorInsertGEM7
UseAAVTagsHaloTagExpressionMutationPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
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pAG Retro EGFP Neo
Plasmid#234464PurposeControl retroviral vector expressing Green Fluorescent Protein. Neomycin selection.DepositorInsertGreen Fluorescent Protein
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMarch 21, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCS2-zH2B-GFP
Plasmid#226561PurposeExpress H2B-GFP in zebrafish.DepositorInserth2b (h2bc1 Zebrafish)
UseSynthetic Biology; Zebrafish expressionTagsEGFPExpressionMutationPromoterAvailable sinceMarch 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAIP1
Plasmid#233685PurposeEngineered SUMO protease expression plasmid designed for efficient protease-mediated elution.DepositorInsertUlp1-like SUMO protease
UseTagsCAHS-107838 and HisTagExpressionBacterialMutationPromoterT7Available sinceMarch 4, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pZP05
Plasmid#232319PurposepZP05 was constructed by cloning the oriFn-repA fragment from pCWU6 into the smaller suicide plasmid pCM-galK, facilitating replication in Fusobacterium nucleatumDepositorInsertOri-repA
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
3'dmDR-CMV-CasRx-bGHpolyA
Plasmid#229556PurposeExpresses CasRx (RfxCas13d) with double processed direct repeats (15nt) and a non-target spacer in between at 3' endDepositorInsertCasRx
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
1203U-U6-gRNA-pCMV-wtCas9-NG-P2A-mcherry-3'dmDR
Plasmid#221448PurposeExpresses wtCas9-NG with double processed direct repeats (15nt) and a non-target spacer in between at 3' end, mCherry tag, and the gRNA targeting HEK293T-site3DepositorInsertswtCas9-NG
mCherry
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLaG2-T7RNAPC
Plasmid#216337PurposeArabinose-inducible PBad promoter driving expression of the anti-eGFP nanobody LaG2 translationally fused to the C-terminal portion (residues 180-883) of T7RNAPDepositorInsertLaG2-T7RNAPC fusion
UseTagsExpressionBacterialMutationg2658a, g3915aPromoterAvailable sinceJan. 17, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBS-ACTB-C200-T7
Plasmid#229850PurposeDonor vector for the ACTB site containing T7 sequence for detection of HR products.DepositorInsertPartial sequence of ACTB gene
UseHr donor vector for human actb gene.TagsExpressionMutationPromoterAvailable sinceJan. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCG-scr
Plasmid#229847PurposeExpresses wild-type Cas9 and scrambled gRNA.DepositorInsertguide RNA with scrambled sequence
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2025AvailabilityAcademic Institutions and Nonprofits only