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We narrowed to 62,913 results for: cloning

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Showing: 3961 - 3980 of 62913 results

  2. pLL3.7-EF-EYFP-YAP1_5SA-PolyA

    Plasmid
    #112285
    Purpose
    Fluorescently labels human YAP1-2gamma isoform (504 aa) 5SA active mutant - serines 61, 109, 127, 164 and 397 (also known as 381 in other isoforms)
    Depositor
    Erik Sahai
    Publication
    Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.
    Insert
    EYFP-YAP1 (YAP1 Human)
    Use
    Lentiviral
    Mutation
    Human YAP1-2gamma with 5 Serines (61, 109, 127, 1…
    Promoter
    EF-1 alpha
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    112285-plasmid-map-sequence-id-227437

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  7. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q23

    Plasmid
    #111726
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
    Depositor
    Cheryl Arrowsmith
    Publication
    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Q23 (polyQ repeat)
    Promoter
    CMV and P10
    Available since
    June 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    111726-plasmid-map-sequence-id-212896

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  8. pLenti Lifeact-mTagBFP2 PuroR

    Plasmid
    #101893
    Purpose
    Lentiviral expression of mTagBFP2-tagged Lifeact with Puromycin selection in cells including neurons
    Depositor
    Ghassan Mouneimne
    Insert
    Lifeact
    Use
    Lentiviral
    Tags
    mTagBFP2
    Promoter
    CMV immediate early
    Available since
    May 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    101893-plasmid-map-sequence-id-211159

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  13. pJSC114 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1) variant

    Plasmid
    #101215
    Purpose
    Bacterial expression plasmid for SpCas9 eSpCas9(1.1) variant
    Depositor
    Jennifer Doudna, Keith Joung
    Publication
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant K848A/K1003A/R1060A
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    K848A, K1003A and R1060A
    Promoter
    T7
    Available since
    Oct. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    101215-plasmid-map-sequence-id-194634

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  15. pCRISPRi_Mxi1_yl_NHEJ

    Plasmid
    #91249
    Purpose
    CRISPRi vector for Yarrowia lipolytica, repressing KU70 and KU80 for enhanced HR
    Depositor
    Ian Wheeldon
    Publication
    Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.
    Inserts
    Codon optimized dCas9-Mxi1
    KU70 sgRNA expression cassette
    KU80a sgRNA expression cassette
    KU80b sgRNA expression cassette
    Use
    CRISPR and Synthetic Biology
    Expression
    Yeast
    Promoter
    SCR1'-tRNA and UAS1B8-TEF(136)
    Available since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    91249-plasmid-map-sequence-id-181276

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  17. STARR-seq luciferase validation vector_ORI_CMV

    Plasmid
    #99312
    Purpose
    Luciferase validation vector with CMV enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity
    Depositor
    Alexander Stark
    Publication
    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453
    Insert
    CMV enhancer
    Use
    Luciferase; Starr-seq luciferase validation vector
    Expression
    Mammalian
    Available since
    Aug. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    99312-plasmid-map-sequence-id-190933

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Showing: 3961 - 3980 of 62913 results