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pET28-p53 (Dog 1-77)

Plasmid

#62060

Purpose

Expression of Dog p53 (residues 1-77) in e. coli

Depositor

Gary Daughdrill

Article

Daughdrill et al PLoS One. 2011;6(12):e29207. doi: 10.137

Insert

p53

Tags

His

Expression

Bacterial

Mutation

contains TAD residues 1-77

Promoter

T7

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pRS413 Prp5 WT

Plasmid

#87599

Purpose

Encodes Prp5 WT for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

PRP5 (PRP5 )

Expression

Bacterial and Yeast

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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Vh-HA-2xDHFR

Plasmid

#20218

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

2xDHFR (folA E. coli)

Tags

HA and Vh

Expression

Mammalian

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
NSK163 CMV-TO-SMN2 Nluc - active (exon 6, 7, 8)

Plasmid

#247276

Purpose

Expresses human SMN2 exons 6, 7, 8 followed by Nluc

Depositor

Xiaojing Gao

Article

Kolber et al bioRxiv 2025.09.18.677213

Insert

SMN2 exons 6,7,8-Nluc (SMN2 Human)

Expression

Mammalian

Mutation

"A" insertion at position 49 of exon 7

Promoter

CMV-TO

Available Since

Nov. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pAttBF3Neo

Plasmid

#244886

Purpose

Neo resistant attB phiC31 recombination-mediation insertion (RMI) vector for C. elegans

Depositor

Michael Nonet

Article

Beck et al MicroPubl Biol. 2025 Sep 2;2025:10.17912

Type

Empty backbone

Use

Phic31 recombinase mediated insertion vector

Expression

Worm

Promoter

none

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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MbCas12a_K487_GPG_GR2

Plasmid

#231577

Purpose

Encodes a cortisol inducible variant of MbCas12a

Depositor

Dominik Niopek

Article

Wolf et al bioRxiv 2024.12.04.626757

Insert

MbCas12a with a GR2 insertion behind K487

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pX330-U6-BRD4-HaloTag-Guide-hspCas9

Plasmid

#228576

Purpose

Expression of Halo-tagged human BRD4 gRNA; CRISPR-mediated gene insertion

Depositor

Marcin Imielinski

Insert

BRD4 gRNA (BRD4 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pReporter_ZF2_ZF1_mNeonGreen

Plasmid

#213834

Purpose

Facilitates genomic integration of the reporter for screen 1: ZF-1 followed by ZF-2 upstream of the reporter gene.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pReporter_ZF1_mNeonGreen_ZF2

Plasmid

#213835

Purpose

Facilitates genomic integration of the reporter for screen 2: ZF-1 upstream of the reporter gene and ZF-2 downstream.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pReporter_ZF1_ZF2_mNeonGreen

Plasmid

#213836

Purpose

Facilitates genomic integration of the reporter for screen 3: ZF-2 followed by ZF-1 upstream of the reporter gene.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pReporter_ZF2_mNeonGreen_ZF1

Plasmid

#213837

Purpose

Facilitates genomic integration of the reporter for screen 4: ZF-2 upstream of the reporter gene and ZF-1 downstream.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pReporter_mNeonGreen

Plasmid

#213838

Purpose

Facilitates genomic integration of the control reporter without zinc finger binding sites.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pPtet-2-VP16

Plasmid

#213842

Purpose

Used for expressing VP16 fused to ZF-2.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

VP16

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pPtet-1-VP16

Plasmid

#213843

Purpose

Used for expressing VP16 fused to ZF-1.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

VP16

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-C-PrLD

Plasmid

#216398

Purpose

Expresses 6xHis-tagged C-terminal prion-like domains of Efg1

Depositor

Nicolas Fawzi

Article

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-C-PrLD

Tags

6xHis-tags

Expression

Bacterial

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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pDonor_Tn[GFP]

Plasmid

#213903

Purpose

Mariner transposon expressing mNeonGreen with kan selection casette

Depositor

James Collins

Article

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

mariner transposon with kan resistance and mNeonGreen

Expression

Bacterial

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pDonor_Tn[RFP]

Plasmid

#213904

Purpose

Mariner transposon expressing mCherry with cam selection casette

Depositor

James Collins

Article

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

mariner transposon with cam resistance and mCherry

Expression

Bacterial

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-SbfI_O

Plasmid

#147156

Purpose

Insect Expression vector

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Type

Empty backbone

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-dCer3-C2-dCer3

Plasmid

#191328

Purpose

Expresses the fluorescently labeled membrane anchor C2 without the chemical dimerization domain FRB as a control for recruiting proteins to plasma membrane

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

dCer3-C2-dCer3

Expression

Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pVY-11 mScarletI-tag3

Plasmid

#194626

Purpose

fluorescent proteins with cationic peptide tags to promote biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al Nat Commun. 2022 Dec 22;13(1):7882. doi:

Insert

mScarlet-I-(GGSKKRKKR)3

Tags

GGSKKRKKRGGSKKRKKRGGSKKRKKR and MGHHHHHGGA

Expression

Bacterial

Promoter

T7

Available Since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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