We narrowed to 7,533 results for: ski

1. pDF0237 pCMV - dead huDisCas7-11 - NES - huADAR2

   Plasmid

   #172510

   Purpose

   Encodes human codon optimized catalytically dead DisCas7-11-NES-huADAR2 fusion protein alone for expression in mammalian cells

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

   Insert

   pCMV - dead huDisCas7-11 - NES - huADAR2
   

   Expression

   Mammalian

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEY43

   Plasmid

   #191047

   Purpose

   eat-4(prom6-1) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

   Depositor

   Oliver Hobert, Eviatar Yemini

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   eat-4(prom6-1) (eat-4 Nematode)
   

   Expression

   Worm

   Mutation

   None

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQE32-pr-mEosFP (mEosFP-V69T)

   Plasmid

   #99213

   Purpose

   Bacterial expression of the primed conversion capable protein pr-mEosFP (mEosFP-V69T).

   Depositor

   Periklis Pantazis

   Article

   Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

   Insert

   pr-mEosFP
   

   Expression

   Bacterial

   Mutation

   monomeric variant with mutation V69T for primed c…

   Promoter

   T5

   Available Since

   June 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV2_hSyn_NES-Caprola_06-mEGFP_WRPE-SV40

   Plasmid

   #194690

   Purpose

   hSyn1 driven expression of the calcium recorder Caprola_06 fused to mEGFP for neuronal expression through AAV transduction

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_06-mEGFP
   

   Use

   AAV

   Tags

   mEGFP

   Promoter

   hSyn1

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. W118-1_hTGM4-Flag

   Plasmid

   #180409

   Purpose

   lentiviral transduction of human TGM4 (with a C-terminal Flag tag) gene

   Depositor

   Huda Zoghbi

   Article

   Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

   Insert

   TGM4 (TGM4 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. W118-1_hTGM5-V5

   Plasmid

   #180414

   Purpose

   lentiviral transduction of human TGM5 (with a C-terminal V5 tag) gene

   Depositor

   Huda Zoghbi

   Article

   Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

   Insert

   TGM5 (TGM5 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. SIN40C.SFFV.dTomato.let-7c

   Plasmid

   #169301

   Purpose

   Constitutive overexpression of let-7c with dTomato as a reporter fluorescent protein

   Depositor

   Jan-Henning Klusmann

   Article

   Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

   Insert

   let-7c (MIRLET7C Human)
   

   Use

   Lentiviral

   Tags

   dTomato

   Promoter

   SFFV

   Available Since

   July 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAVS1-SA-neo-CAGGS-PE2-2A-GFP

   Plasmid

   #180014

   Purpose

   Targeting vector for knocking in constitutively expressed PE2-2A-GFP into the human AAVS1 locus

   Depositor

   Dirk Hockemeyer

   Article

   Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

   Insert

   PE2-2A-GFP
   

   Use

   CRISPR; Human genome engineering

   Tags

   GFP

   Promoter

   CAG

   Available Since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. BPTF PHD and Bromodomain

   Plasmid

   #92101

   Purpose

   Expresses GST fusion PHD and Bromodomain from BPTF protein

   Depositor

   Albert Jeltsch

   Article

   Mauser et al Biochimie. 2018 Mar;146:14-19. doi: 10.1

   Insert

   PHD and Bromodomain (BPTF Human)
   

   Tags

   GST Tag

   Expression

   Bacterial

   Promoter

   tac promoter

   Available Since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEND-400_Cacnes_CAMP2

    Plasmid

    #225606

    Purpose

    Suicide C. acnes vector derivative of pEND-350 optimized for recombination into the CAMP2 locus, with BxbI recombination sites flanking EryR resistance cassette

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. mMaple-OMP25

    Plasmid

    #141151

    Purpose

    Photoconvertible fluorescent protein targeted to the outer mitochondrial membrane (OMM) in mammalian cells.

    Depositor

    Gia Voeltz

    Article

    Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    OMP25 (Synj2bp Rat)
    

    Tags

    mMaple

    Expression

    Mammalian

    Mutation

    OMP25 c terminus from addgene plasmid #69598

    Promoter

    CMV

    Available Since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pWZLblasti-HA-ERK5

    Plasmid

    #53175

    Purpose

    Expresses HA tagged human ERK5 from blasticidin resistance retroviral vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    ERK5 (MAPK7 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Available Since

    June 24, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pRSET-pr-mEos3.2 (mEosFP3.2-A69T)

    Plasmid

    #99224

    Purpose

    Bacterial expression of the primed conversion capable protein pr-mEos3.2 (mEos3.2-A69T).

    Depositor

    Periklis Pantazis

    Article

    Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

    Insert

    pr-mEos3.2
    

    Expression

    Bacterial

    Mutation

    A69T for primed conversion applications

    Promoter

    T7

    Available Since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. MAC3-FGFR1

    Plasmid

    #222611

    Purpose

    Expression of MAC3-tagged FGFR1

    Depositor

    Markku Varjosalo

    Article

    Liu et al Methods Mol Biol. 2023;2690:281-297. doi

    Insert

    FGFR1 (FGFR1 Human)
    

    Tags

    MAC3

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Aug. 5, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pcDNA SF-HERC2 F2

    Plasmid

    #55785

    Purpose

    Expresses N-terminal 3xFLAG/STREP tagged HERC2 (950-1750aa)

    Depositor

    David Chan

    Article

    Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

    Insert

    3xFLAG tagged HERC2 (950aa-1750aa) (HERC2 Human)
    

    Tags

    3xFLAG and STREP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 23, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pBAD-eLACCO2.1

    Plasmid

    #208017

    Purpose

    Biosensor for extracellular L-lactate

    Depositor

    Robert Campbell

    Article

    Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

    Insert

    eLACCO2.1
    

    Expression

    Bacterial

    Available Since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. GA-MFF

    Plasmid

    #141160

    Purpose

    Dimerization-dependent green fluorescent protein targeted to mitochondrial outer membrane

    Depositor

    Gia Voeltz

    Article

    Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    mitochondrial fission factor (MFF Human)
    

    Tags

    GA

    Expression

    Mammalian

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDF0250 DisCas7-11 full locus with Golden gate acceptor site for spacer

    Plasmid

    #172501

    Purpose

    Encodes the DisCas7-11 full locus along with a DR and golden gate site for cloning spacers

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    DisCas7-11 full locus with Golden gate acceptor site for spacer
    

    Expression

    Bacterial

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. Rescue Septin 11 mRNA

    Plasmid

    #122022

    Purpose

    Rescue mRNA for Septin 11 Knock Down with sh1 shRNA. Splice variant II (EU711415) with 5 silent mutations at the SEPT11 sh1 shRNA target site.

    Depositor

    Angel de Blas

    Article

    Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

    Insert

    SEPT11 (Sept11 Rat)
    

    Expression

    Mammalian

    Mutation

    Silent mutations are in 5 consecutive codons (AUU…

    Available Since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-51b(+)-Caprola_05

    Plasmid

    #194666

    Purpose

    Protein production of the split HaloTag based calcium recorder Caprola_05 in bacteria

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_05
    

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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