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We narrowed to 55 results for: manf

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pCS2+ HA BEX3

Plasmid

#231004

Purpose

Transient overexpression of human BEX3 in mammalian cells

Depositor

Michael Rape

Article

Manford et al Cell. 2021 Oct 14;184(21):5375-5390.e16.

Insert

BEX3 (BEX3 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2 3xFLAG FEM1A

Plasmid

#231001

Purpose

Transient overexpression of mouse FEM1A in mammalian cells

Depositor

Michael Rape

Article

Manford et al Cell. 2021 Oct 14;184(21):5375-5390.e16.

Insert

FEM1A (Fem1a Mouse)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1 ElonginB, ElonginC

Plasmid

#230999

Purpose

Dual expression vector containing mouse ElonginB and ElonginC for E.coli protein purification

Depositor

Michael Rape

Article

Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

Inserts

Elongin B (Elob Mouse)
Elongin C (Eloc Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+ E4F1 degron(220-242)-GFP-IRES-mCherry

Plasmid

#231007

Purpose

Protein stability reporter construct for E4F1 consisting of aa 220-242 for transient overexpression in mammalian cells.

Depositor

Michael Rape

Article

Manford et al Cell. 2021 Oct 14;184(21):5375-5390.e16.

Insert

E4F1 aa220-242 (E4F1 Human)

Use

Tags

AcGFP1

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+ GFP-CDK5R1 degron (283-307)-IRES-mCherry

Plasmid

#231008

Purpose

Protein stability reporter construct for CDK5R1 consisting of aa 283-307 for transient overexpression in mammalian cells.

Depositor

Michael Rape

Article

Manford et al Cell. 2021 Oct 14;184(21):5375-5390.e16.

Insert

CDK5R1 aa283-307 (CDK5R1 Human)

Use

Tags

AcGFP1

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac Dual His6-TEV-CUL2, Rbx1

Plasmid

#231000

Purpose

Dual expression vector containing mouse CUL2 and Rbx1 for protein puritification from SF9 insect cells

Depositor

Michael Rape

Article

Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

Inserts

Rbx1 (Rbx1 Mouse)
CUL2 (Cul2 Mouse)

Use

Tags

6x HIS

Expression

Insect

Mutation

Promoter

Available since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pRS424GFP-2xPH(Osh2)

Plasmid

#36095

Depositor

Scott Emr

Article

Stefan et al Cell. 2011 Feb 4;144(3):389-401.

Insert

2xPH(Osh2) fusion

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

PRC1

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only
pLV.PGK.GFP.miR9T

Plasmid

#115973

Purpose

miR-9 regulated GFP expression

Depositor

Johan Jakobsson

Article

Akerblom et al Nat Commun. 2013;4:1770. doi: 10.1038/nc

Insert

GFPmiR9T

Use

Lentiviral

Tags

GFP

Expression

Mutation

Promoter

PGK

Available since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pSCW01

Plasmid

#72300

Purpose

Starting substrate for in vitro DNA mismatch repair substrate monitored by PstI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.

Depositor

Peggy Hsieh

Article

Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

Type

Empty backbone

Use

Derivative of puc19

Tags

Expression

Bacterial

Mutation

Promoter

lac

Available since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pSCW02

Plasmid

#72301

Purpose

Starting substrate for in vitro DNA mismatch repair substrate monitored by FauI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.

Depositor

Peggy Hsieh

Article

Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

Type

Empty backbone

Use

Derivative of puc19

Tags

Expression

Bacterial

Mutation

Promoter

lac

Available since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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