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Plasmid#113194PurposeEncodes gRNA for human AAVS1DepositorUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only
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AAVS1-TALEN-L
Plasmid#59025PurposeTALEN designed to target the human AAVS1/PPP1R12C locusDepositorInsertAAVS1-TALEN-L
UseAAV and TALENTags3xFlag and NLSExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TALEN-R
Plasmid#59026PurposeTALEN designed to target the human AAVS1/PPP1R12C locusDepositorInsertAAVS1-TALEN-R
UseAAV and TALENTags3xFlag and NLSExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
Hygro-iDEST
Plasmid#75339PurposeGATEWAY destination vector for site-specific integration into the human AAVS1/PPP1R12C locus for doxycycline-inducible gene over-expressionDepositorInsertTRE-DEST
UseTagsExpressionMammalianMutationPromoterTRE-tightAvailable sinceMay 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
Puro-iDEST
Plasmid#75336PurposeGATEWAY destination vector for site-specific integration into the human AAVS1/PPP1R12C locus for doxycycline-inducible gene over-expressionDepositorInsertTRE-DEST
UseTagsExpressionMammalianMutationPromoterTRE-tightAvailable sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
Puro-Cas9 donor
Plasmid#58409PurposeDonor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locusDepositorInserthSpCas9
UseCRISPRTags3xFlag, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationPromoterAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-TLR6
Plasmid#118997PurposeTargeting vector for human AAVS1 with TLR6 reporter insertDepositorUseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZT-AAVS1-R1
Plasmid#52638PurposeTALEN-mediated gene editing at human PPP1R12C/AAVS1 locusDepositorInsertRight AAVS1 TALEN
UseTALENTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pZT-AAVS1-L1
Plasmid#52637PurposeTALEN-mediated gene editing at human PPP1R12C/AAVS1 locusDepositorInsertLeft AAVS1 TALEN
UseTALENTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
Hygro-Cas9 donor
Plasmid#86883PurposeDonor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus with hygromycin selectionDepositorInserthSpCas9
UseCRISPRTags3xFlag, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationPromoterAvailable sinceMarch 2, 2017AvailabilityAcademic Institutions and Nonprofits only