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Plasmid#68178PurposeProvides the 5'-untranslated region of the Arabidopsis thaliana actin2 gene as a level 0 GoldenBraid partDepositorInsert5'UTR AtAct2
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEF-GalNAcT2-moxGFP2
Plasmid#218966PurposeExpresses full length GalNAcT2-moxGFP2 mammalian cellsDepositorInsertGalNAcT2-moxGFP2 (GALNT2 Human)
UseTagsmoxGFP2ExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACT2 V1H (P#663)
Plasmid#14665DepositorInsertV1H (ATP6V1H Human)
UseTagsGal4 ADExpressionYeastMutationPromoterAvailable sinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA CASTOR2 ACT2
Plasmid#84494Purposeoverexpression of CASTOR2 ACT2 domain (aa 171-end)DepositorInsertCASTOR2 ACT2 (GATSL2 Human)
UseTagsHAExpressionMammalianMutation171-last amino acidPromoterCMVAvailable sinceOct. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA CASTOR1 ACT2
Plasmid#84492Purposeoverexpression of CASTOR1 ACT2 domain (aa 169-end)DepositorInsertCASTOR1 ACT2 (GATSL3 Human)
UseTagsHAExpressionMammalianMutation169-last amino acidPromoterCMVAvailable sinceOct. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEF-GalNAcT2-mGold
Plasmid#218964PurposeExpresses full length GalNAcT2-mGold in mammalian cellsDepositorInsertGalNAcT2-mGold (GALNT2 Human)
UseTagsmGoldExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF-GalNAcT2-msYFP2
Plasmid#218963PurposeExpresses full length GalNAcT2-msYFP2 in mammalian cellsDepositorInsertGalNAcT2-msYFP2 (GALNT2 Human)
UseTagsmsYFP2ExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF-GalNAcT2-msGFP2
Plasmid#218965PurposeExpresses full length GalNAcT2-msGFP2 mammalian cellsDepositorInsertGalNAcT2-msGFP2 (GALNT2 Human)
UseTagsmsGFP2ExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACT2-μ1B (137-423)
Plasmid#198172PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-1 μ1B (137-423) fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-1 μ1B (137-423)
UseTagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationPromoterADH1Available sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pICH47811-pAtAct2::Cas9-nls::tSlATPase
Plasmid#160221PurposeCas9-nuclear localization, pACT2 promoter, SlATPase terminator transcriptional unit, inserted at the MoClo toolkit level 1 acceptor position 2 reverseDepositorInsertpAtACT2::Cas9-nls::tSlATPase
UseSynthetic Biology; Moclo toolkit level 1 acceptor…TagsExpressionPlantMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
bact2-mKate2-P2A-TurboID-dVHH[GFP]
Plasmid#163857PurposeZebrafish ubiquitous specific expression of mKate2-P2A-TurboID-dVHH[GFP]. Parton lab clone JBHDepositorInsertTurboID-VHH[EGFP]
UseZebrafish expressionTagsmKate2-P2A-TurboIDExpressionMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
Bact2-APEX2-GBP in pDEST-Tol2-pA2-acrys-cherry
Plasmid#67668PurposeZebrafish expression vector for ubiquitous expression of APEX2-GBPDepositorInsertAPEX2-GBP
UseZebrafish expression with tol2TagsExpressionMutationPromoterAvailable sinceDec. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
KBB221
Plasmid#185071PurposeSST::ACT2 "SA236"DepositorInsertACT2
UseTagsExpressionYeastMutationACT2-SA236PromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP721_pSP14
Plasmid#192434PurposeTo test the relative expression strength of the Act2 promoterDepositorInsertAct2::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP792_L2_FRT-OCS-tGFP_Red_mCherry_HSP-FlpO
Plasmid#192382PurposeTo test if a single plasmid stably transformed into Arabidopsis can switched from off to on when heat shocked.DepositorInsertAct2::B3RT-OCS-B3RT::Act2::FRT-OCS-FRT::Turbo GFP
UseSynthetic BiologyTagsN7ExpressionPlantMutationPromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pICH50581
Plasmid#50256DepositorInsertpromoter, act2 (AT3G18780, Arabidopsis thaliana)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pICSL70002
Plasmid#50333DepositorInsertBiotin Selection Cassette (Act2 promoter (A. thaliana) + BirA (E.coli) + 35s terminator (Cauliflower Mosaic Virus))
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pICH44300
Plasmid#50340DepositorInsert3'UTR, polyadenylation signal/terminator, act2 (A. thaliana)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pICH43844
Plasmid#50329DepositorInsertCDS, phosphinothricin acetyl transferase, (S. hygroscopicus) with intron from ACT2 (A.thaliana)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pICH87644
Plasmid#50274DepositorInsertpromoter, act2 (AT3G18780, A. thaliana) and 5'UTR, omega (Tobacco Mosaic Virus)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceMarch 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
LLP743_L2pV1_OR_B3RT_FRT_Rluc_0I_lacZ
Plasmid#192387PurposeTo act as the 0-input state of the OR gate.DepositorInsertAct2::FRT-B3RT-OCS-FRT-B3RT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP689_L2_1IF2_FRT-OCS_Rluc_C1_FlpF2
Plasmid#192412PurposeTo act as a 1-input state of the split-AND gate.DepositorInsertAct2::FRT-OCS-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP690_L2_1IF1_FRT-OCS_Rluc_FlpF1_C1-NLS
Plasmid#192413PurposeTo act as a 1-input state of the split-AND gate.DepositorInsertAct2::FRT-OCS-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP752_L2pV1_AND_B3RT_FRT_Rluc_2IBF_lacZ
Plasmid#192396PurposeTo act as a 2-input state of the AND gate.DepositorInsertAct2::FRT-OCS-FRT::B3RT-OCS-B3RT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP747_L2pV1_OR_B3RT_FRT_Rluc_2IBF_lacZ
Plasmid#192391PurposeTo act as a 2-input state of the OR gate.DepositorInsertAct2::FRT-B3RT-OCS-FRT-B3RT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP675_L2_pV01_1I_vox-OCS-Rluc_TCTP_Vika_lacZ
Plasmid#192427PurposeTo act as the on state for testing of the alt Vika recombinase.DepositorInsertAct2::vox-OCS-vox::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP772_L2pV1_NOR_B3RT_FRT_Rluc_2IBF_lacZ
Plasmid#192432PurposeTo act as a 2-input state of the NOR gate.DepositorInsertAct2::FRT-B3RT-Rluc-B3RT-FRT
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP765_L2pV3_ANIMPLYB_B3RT_FRT_OCS_Rluc_1IF_lacZ
Plasmid#192409PurposeTo act as a 1-input state of the A NIMPLY B gate.DepositorInsertB3RT-Act2-B3RT::FRT-OCS-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceSept. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
LLP744_L2pV1_OR_B3RT_FRT_Rluc_1IF_lacZ
Plasmid#192388PurposeTo act as a 1-input state of the OR gate.DepositorInsertAct2::FRT-B3RT-OCS-FRT-B3RT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
LLP673_L2_pV01_1I_VloxP-OCS-Rluc_TCTP_VCre_lacZ
Plasmid#192425PurposeTo act as the on state for testing of the alt VCre recombinase.DepositorInsertAct2::VloxP-OCS-VloxP::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only