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Showing: 31 - 58 of 58 results

  1. pHJ42

    Plasmid
    #48359
    Purpose
    Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Bleomycin/Gancyclovir selectable
    Depositor
    George Cross
    Article
    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1
    Insert
    Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-BLE-Ty1-TK-loxP-3' ALD UTR
    Use
    Cre/Lox; Knockout in t. brucei
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    48359-plasmid-map-sequence-id-70049

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  5. LLP340 pEF1a-NLS-scFvGCN4-DNMT3a (mutant)

    Plasmid
    #100944
    Purpose
    Single chain variable fragment antibody GCN4 fused to DNMT3a catalytic mutant, binds SunTag, with 3xTy1 tag
    Depositor
    Ryan Lister
    Article
    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.
    Insert
    scFvGCN4, sfGFP, GB1, DNMT3a catalytic mutant (DNMT3A Synthetic, Human)
    Use
    Tags
    3xTy1
    Expression
    Mammalian
    Mutation
    F636A, E660A, E725A, R295A
    Promoter
    Available since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    100944-plasmid-map-sequence-id-206112

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  20. LLP618_αGCN4-KRAB-CO

    Plasmid
    #211782
    Purpose
    SunTag counterpart binding domain, aGCN4, fused to transcriptional repressor KRAB, with GFP selection
    Depositor
    Ryan Lister
    Article
    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.
    Insert
    aGCN4-mCherry
    Use
    Tags
    3xTy1
    Expression
    Mammalian
    Mutation
    Last 300 bp codon-optimised (CO) to detect mCherr…
    Promoter
    pEF1a and pSV40
    Available since
    Dec. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    211782-plasmid-map-sequence-id-417156

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  23. LLP779_αGCN4-VP64-CO

    Plasmid
    #211772
    Purpose
    SunTag counterpart binding domain, αGCN4, fused to transcriptional activator VP64, with GFP selection
    Depositor
    Ryan Lister
    Article
    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.
    Insert
    aGCN4-VP64
    Use
    Tags
    3xTy1
    Expression
    Mammalian
    Mutation
    Last 300 bp codon-optimised (CO) to detect VP64 e…
    Promoter
    pEF1a and pSV40
    Available since
    Dec. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    211772-plasmid-map-sequence-id-417389

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  27. LLP185 pLVP-dCas9-DNMT3a V2

    Plasmid
    #100936
    Purpose
    dCas9 and DNMT WT on C terminus, 4 NLS, driven by pGK promoter, with P2A site and PURO gene, within a lentiviral transfer backbone
    Depositor
    Ryan Lister
    Article
    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.
    Insert
    dCas9, DNMT3a catalytic domain (DNMT3A Human, S.pyogenes)
    Use
    CRISPR and Lentiviral
    Tags
    3xHA and 3xTy1
    Expression
    Mammalian
    Mutation
    D10A, H840A for dCas9
    Promoter
    Available since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    100936-plasmid-map-sequence-id-206124

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Showing: 31 - 58 of 58 results