We narrowed to 67 results for: Geobacillus
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pET28a-ybbR-HIS-Xyl-DocI
Plasmid#58711PurposeCharacteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tagDepositorInsertXyl-DocI
TagsHIS, HRV 3C, and ybbRExpressionBacterialMutationchanged Threonin 120 to Cysteine in Xylanase Doma…PromoterT7Available SinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pTCas9_scr
Plasmid#207566PurposeThermoCas9-mediated cleavage of genomic DNA in E. coliDepositorInsertPlac/tet_ThermoCas9_PlacI_lacI_PJ23119_sgRNA(scrambled non-targeting spacer)
ExpressionBacterialMutationWild-typeAvailable SinceDec. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGCas9_scr
Plasmid#207567PurposeGeoCas9-mediated cleavage of genomic DNA in E. coliDepositorInsertPlac/tet_GeoCas9_PlacI_lacI_PJ23119_sgRNA(scrambled non-targeting spacer)
ExpressionBacterialMutationWild-typeAvailable SinceDec. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pdTCas9_scr
Plasmid#207568PurposedThermoCas9-mediated silencing of genomic gene in E. coliDepositorInsertPlac/tet_dThermoCas9_PlacI_lacI_PJ23119_sgRNA(scrambled non-targeting spacer)
ExpressionBacterialMutationThermoCas9(D8A,H582A)Available SinceDec. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pdGCas9_scr
Plasmid#207569PurposedGeoCas9-mediated silencing of genomic gene in E. coliDepositorInsertPlac/tet_dGeoCas9_PlacI_lacI_PJ23119_sgRNA(scrambled non-targeting spacer)
ExpressionBacterialMutationGeoCas9(D8A,H582A)Available SinceDec. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAcrTCas9_scr
Plasmid#207571PurposeAcrIIC1-mediated inhibition of ThermoCas9 cleavage activity in E. coliDepositorInsertPrha_AcrIIC1Nme_Plac/tet_ThermoCas9_PlacI_lacI_PJ23119_sgRNA(scrambled non-targeting spacer)
ExpressionBacterialMutationWild-typeAvailable SinceDec. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAcrGCas9_scr
Plasmid#207572PurposeAcrIIC1-mediated inhibition of GeoCas9 cleavage activity in E. coliDepositorInsertPrha_AcrIIC1Nme_Plac/tet_GeoCas9_PlacI_lacI_PJ23119_sgRNA(scrambled non-targeting spacer)
ExpressionBacterialMutationWild-typeAvailable SinceDec. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
enDR3-TEV-mEGFP-NLS (pRS1451)
Plasmid#240546PurposeExpression of the engineered N-terminal hybrid-binding domain of RNase H3 (enDR3) for capturing R-loops. A TEV cleavage site, mEGFP and nuclear localization signal (NLS) are included in the fusion.DepositorInsertenDR3
ExpressionMammalianMutationL52MAvailable SinceAug. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
nb-enDR3-TEV-mEGFP-NLS (pRS1458)
Plasmid#240547PurposeExpression of negative control for R-loops capture experiments using enDR3. A mutated, non-R-loops binding version of enDR3 is expressed in fusion with the TEV cleavage site, mEGFP, and NLS.DepositorInsertnb-enDR3
ExpressionMammalianMutationS48A, K50AAvailable SinceAug. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
Catalytically Enhanced (E655G and N696I) -ThermoCas9-3xNLS
Plasmid#254684PurposeExpresses Catalytically Enhanced (E655G and N696I) ThermoCas9 with 3x NLS tags. Utilized for RNP transfectionDepositorInsertCatalytically Enhanced (E655G and N696I) ThermoCas9
TagsNLS, SV40 NLS, and nucleoplasmin NLSExpressionBacterialAvailable SinceMay 6, 2026AvailabilityAcademic Institutions and Nonprofits only -
Wild-type-ThermoCas9-3xNLS
Plasmid#254685PurposeExpresses Wild-type ThermoCas9 with 3x NLS tags. Utilized for RNP transfectionDepositorInsertWildtype ThermoCas9
TagsNLS, SV40 NLS, and nucleoplasmin NLSExpressionBacterialAvailable SinceApril 27, 2026AvailabilityAcademic Institutions and Nonprofits only -
EcNGsC-MutY-pKK223
Plasmid#131376PurposeLow level expression of E.coli G.stearothermophillus MutY chimera proteinDepositorInsertEcNGsC MutY chimera
ExpressionBacterialMutationFusion of two MutY genes. Residues 1-225 are from…PromotertacAvailable SinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
EcNGsC-S308P-H309D-pKK223
Plasmid#131378PurposeLow level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid changes S308P and H309D in Gs MutY.DepositorInsertEcNGsC MutY chimera S308P H309D
ExpressionBacterialMutationFusion of two MutY genes. Residues 1-225 are from…PromotertacAvailable SinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
EcNGsC-F307A-S308A-pKK223
Plasmid#131379PurposeLow level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid changes F307A and S308A in Gs MutY.DepositorInsertEcNGsC MutY chimera F307A S308A
ExpressionBacterialMutationFusion of two MutY genes. Residues 1-225 are fro…PromotertacAvailable SinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
EcNGsC-F307Y-pKK223
Plasmid#131381PurposeLow level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid change F307Y in Gs MutY.DepositorInsertEcNGsC MutY chimera F307Y
ExpressionBacterialMutationFusion of two MutY genes. Residues 1-225 are from…PromotertacAvailable SinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
EcNGsC-F307A-pKK223
Plasmid#131382PurposeLow level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid change F307A in Gs MutY.DepositorInsertEcNGsC MutY chimera F307A
ExpressionBacterialMutationFusion of two MutY genes. Residues 1-225 are from…PromotertacAvailable SinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
EcNGsC-S308V-pKK223
Plasmid#131393PurposeLow level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid change S308V in Gs MutY.DepositorInsertEcNGsC MutY chimera S308V
ExpressionBacterialMutationFusion of two MutY genes. Residues 1-225 are fro…PromotertacAvailable SinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
EcNGsC-S308A-pKK223
Plasmid#131394PurposeLow level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid change S308A in Gs MutY.DepositorInsertEcNGsC MutY chimera S308A
ExpressionBacterialMutationFusion of two MutY genes. Residues 1-225 are fro…PromotertacAvailable SinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
EcNGsC-DelFSH(307-309)-pKK223
Plasmid#131377PurposeLow level expression of E.coli G.stearothermophillus MutY chimera protein with deletion of residues FSH 307-309 in Gs MutY.DepositorInsertEcNGsC MutY chimera DelFSH(307-309)
ExpressionBacterialMutationFusion of two MutY genes. Residues 1-225 are from…PromotertacAvailable SinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMM7
Plasmid#201213PurposeIntegrative GFP-based shuttle vector for genetic engineering of Parageobacillus thermoglucosidasius. Carries homologous flanks for deletion of key sporulation regulator spo0A.DepositorInsertssfGFP
Spo0A right flank
Spo0A left flank
UseSynthetic BiologyExpressionBacterialMutationF11V, T13P, N39D, A179AAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only
