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pDONR223_RBM10_p.E4K

Plasmid

#82860

Purpose

Gateway Donor vector containing RBM10, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

RBM10 (RBM10 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

E4K; E849D

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RBM10_p.Y580F

Plasmid

#82834

Purpose

Gateway Donor vector containing RBM10 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

RBM10 (RBM10 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

Y580F

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pkk223_RbMutY_D146N_E45Q

Plasmid

#210798

Purpose

pkk223 with Rhodobacteraceae MutY containing D146N and E45Q mutations to reduce catalytic activity

Depositor

Martin Horvath

Article

Utzman et al PLoS One. 2024 May 8;19(5):e0284642. doi

Insert

MutY (adenine glycosylase)

Use

Tags

Expression

Bacterial

Mutation

D146N_E45Q

Promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pkk223_RbMutY_F298A_T299A_H300A

Plasmid

#210799

Purpose

pkk223 with Rhodobacteraceae MutY containing F298A, S299A, and H300A mutations to reduce recognition of OG:A

Depositor

Martin Horvath

Article

Utzman et al PLoS One. 2024 May 8;19(5):e0284642. doi

Insert

MutY (adenine glycosylase)

Use

Tags

Expression

Bacterial

Mutation

F298A_T299A_H300A

Promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pkk223_RbMutY

Plasmid

#210797

Purpose

pkk223 with Rhodobacteraceae MutY insert

Depositor

Martin Horvath

Article

Utzman et al PLoS One. 2024 May 8;19(5):e0284642. doi

Insert

MutY (adenine glycosylase)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_RBM28_PRRT4

Plasmid

#205909

Purpose

Express mEGFP-tagged fusion protein, RBM28_PRRT4 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

RBM28_PRRT4 (RBM28, PRRT4 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_ITGB6_RBMS1

Plasmid

#205831

Purpose

Express mEGFP-tagged fusion protein, ITGB6_RBMS1 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

ITGB6_RBMS1 (ITGB6, RBMS1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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RBMX2-1

Plasmid

#155915

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

RBMX2 (RBMX2 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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RBM8A

Plasmid

#155910

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

RBM8A (RBM8A Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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RBM41

Plasmid

#155902

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

RBM41 (RBM41 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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RBM22

Plasmid

#155889

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

RBM22 (RBM22 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223_RBM34_WT_V5

Plasmid

#82998

Purpose

Gateway Donor vector containing RBM34, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

RBM34 (RBM34 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

deleted amino acids 1-5

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RBM34_WT

Plasmid

#82929

Purpose

Gateway Donor vector containing RBM34, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

RBM34 (RBM34 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

deleted amino acids 1-5

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RBM11_p.R74C

Plasmid

#81352

Purpose

Gateway Donor vector containing RBM11 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RBM11 (RBM11 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

R74C

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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RBMS2_2xRRM_pGEX

Plasmid

#135132

Purpose

Recombinant expression of RBMS2 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

RBMS2 (RBMS2 Human)

Use

Tags

GST-SBP

Expression

Bacterial

Mutation

Promoter

tac

Available since

Availability

Academic Institutions and Nonprofits only

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pEGFPN1-RBM3

Plasmid

#87860

Purpose

Expresses human RBM3 coupled to GFP

Depositor

Maria Alvarado-Kristensson

Article

Rossello et al Heliyon. 2016 Sep 24;2(9):e00166. eColle

Insert

RNA binding motif protein 3 (RBM3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

NA

Promoter

Available since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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RBM4_2xRRM-ZNF_pGEX

Plasmid

#135154

Purpose

Recombinant expression of RBM4 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

RBM4 (RBM4 Human)

Use

Tags

GST-SBP

Expression

Bacterial

Mutation

Promoter

tac

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV3Tag8li-hRBM34-HA

Plasmid

#165464

Purpose

Human RBM34 mammalian expression vector with C-terminal HA tag.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

RNA binding motif protein 34 (RBM34 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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RBM45_Full-length_pGEX

Plasmid

#135127

Purpose

Recombinant expression of RBM45 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

RBM45 (RBM45 Human)

Use

Tags

GST-SBP

Expression

Bacterial

Mutation

Promoter

tac

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX RBM42-HA

Plasmid

#229500

Purpose

Lentiviral expression vector to express RBM42 isoform 1 with HA-tag.

Depositor

Davide Ruggero

Article

Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/

Insert

RBM42 (RBM42 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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