We narrowed to 259 results for: T7 rna polymerase

1. pQE30-His-T7RNAP

   Plasmid

   #124138

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   T7 RNA polymerase (T7p07 Enterobacteria phage T7)
   

   Tags

   6x-His

   Expression

   Bacterial

   Available Since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT7RNAP

   Plasmid

   #170101

   Purpose

   expresses T7 RNAP

   Depositor

   Chase Beisel

   Article

   Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

   Insert

   T7-RNA polymerase
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   p70a

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOpen-T7gene1

   Plasmid

   #165505

   Purpose

   Highly processive DNA-dependent RNA polymerase derived from T7 bacteriophage

   Depositor

   Drew Endy, FreeGenes Project, Jennifer Molloy

   Insert

   T7 RNA Polymerase
   

   Use

   Synthetic Biology

   Available Since

   July 27, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pTara:500

   Plasmid

   #60717

   Purpose

   Expresses T7 RNAP from the arabinose inducible promoter PBAD with a RBS strength of 500 from the Salis RBS calculator. Also includes a constitutive araC ORF

   Depositor

   Matthew Bennett

   Article

   Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

   Insert

   T7 RNAP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PBAD

   Available Since

   Nov. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTARA

   Plasmid

   #31491

   Depositor

   Kathleen Matthews

   Article

   Wycuff et al Anal Biochem. 2000 Jan 1. 277(1):67-73.

   Insert

   RNA polymerase
   

   Expression

   Bacterial

   Mutation

   N823D

   Available Since

   June 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. C95m

   Plasmid

   #121001

   Purpose

   MoClo golden gate assembly CD part for T7 RNA polymerase (use with P18m). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   T7 RNA polymerase (Andy please check!)
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTHSSd_14

   Plasmid

   #59952

   Purpose

   Triple split (at 67, 601-SZ)

   Depositor

   Christopher Voigt

   Article

   Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

   Insert

   Three-fragment T7* RNA polymerase
   

   Expression

   Bacterial

   Mutation

   N/A

   Promoter

   PTac

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTHSSd_18

   Plasmid

   #59953

   Purpose

   Quad split (at 61, 179, 601-SZ)

   Depositor

   Christopher Voigt

   Article

   Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

   Insert

   Four-fragment T7* RNA polymerase
   

   Expression

   Bacterial

   Mutation

   N/A

   Promoter

   PTac

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAIP4_5EF9

   Plasmid

   #234154

   Purpose

   Heterologous protein expression of polymerase basic protein 2 (RNA-directed RNA polymerase subunit P3) in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   5EF9 (PB2 Influenza B virus (B/Lee/1940))
   

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pGEMABC

    Plasmid

    #45398

    Depositor

    Katsu Murakami

    Article

    Murakami et al J Biol Chem. 2013 Mar 29;288(13):9126-34

    Inserts

    rpoA (rpoA Escherichia coli)
    rpoB (rpoB Escherichia coli)
    rpoC (rpoC Escherichia coli)
    

    Expression

    Bacterial

    Promoter

    None and T7

    Available Since

    June 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pQE 80L Kan T7WT

    Plasmid

    #102790

    Purpose

    Expresses T7 RNA polymerase

    Depositor

    Andrew Ellington

    Article

    Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

    Insert

    T7 RNA polymerase
    

    Expression

    Bacterial

    Available Since

    July 3, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pAB213

    Plasmid

    #101677

    Purpose

    mCherry under T7 promoter control

    Depositor

    Mustafa Khammash

    Article

    Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

    Insert

    mCherry under T7 promoter control
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAB50

    Plasmid

    #101678

    Purpose

    mCherry under T7 promoter control

    Depositor

    Mustafa Khammash

    Article

    Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

    Insert

    mCherry under T7 promoter control
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTHSSd_41

    Plasmid

    #59964

    Purpose

    High full length T7 RNAP* expression

    Depositor

    Christopher Voigt

    Article

    Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

    Insert

    T7* RNAP
    

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    PJ23105

    Available Since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAB143

    Plasmid

    #101679

    Purpose

    LAA-tagged mCherry under T7 promoter control

    Depositor

    Mustafa Khammash

    Article

    Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

    Insert

    LAA-tagged mCherry under T7 promoter control
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pQE-WT-CGG-R12-KIR

    Plasmid

    #63626

    Purpose

    CGG-R12-KIR RNAP mutant

    Depositor

    Andrew Ellington

    Article

    Meyer et al ACS Synth Biol. 2014 Oct 7.

    Insert

    K1F IKR RNAP
    

    Mutation

    Q744K, L747V, N748H, L749I, R756E, L757M, H772R

    Promoter

    T5/Lac

    Available Since

    July 29, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pQE-WT-CGG-R12-KIRV

    Plasmid

    #63668

    Purpose

    CGG-R12-KIRV RNAP mutant

    Depositor

    Andrew Ellington

    Article

    Meyer et al ACS Synth Biol. 2014 Oct 7.

    Insert

    CGG-R12-KIRV RNAP
    

    Mutation

    Q744K, L747V, N748H, L749I, R756E, L757M, H772R, …

    Promoter

    T5/Lac

    Available Since

    April 15, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pQE-WT-T3-R5-RV

    Plasmid

    #63628

    Purpose

    T3-R5-RV RNAP mutant

    Depositor

    Andrew Ellington

    Article

    Meyer et al ACS Synth Biol. 2014 Oct 7.

    Insert

    T3-R5-RV RNAP
    

    Mutation

    T745K, N748D, L749M, M750I, H772R, E775V

    Promoter

    T5/Lac

    Available Since

    April 15, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pQE-WT-T3-R5-RRVH

    Plasmid

    #63623

    Purpose

    T3-R5-RRVH RNAP mutant

    Depositor

    Andrew Ellington

    Article

    Meyer et al ACS Synth Biol. 2014 Oct 7.

    Insert

    T3 RRVH RNAP
    

    Mutation

    T745K, N748D, L749M, M750I, G753R, H772R, E775V, …

    Promoter

    T5/Lac

    Available Since

    July 29, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pQE-WT-KIF-R5-IRH

    Plasmid

    #63629

    Purpose

    K1F-R5-IRH RNAP mutant

    Depositor

    Andrew Ellington

    Article

    Meyer et al ACS Synth Biol. 2014 Oct 7.

    Insert

    KIF-R5-IRH RNAP
    

    Mutation

    L749I, Q754S, R756N, I761V, H772R, and Q786H

    Promoter

    T5/Lac

    Available Since

    June 1, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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