We narrowed to 1,667 results for: abat

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pLJM60-S6K1(wt) barcoded

Plasmid

#48801

Purpose

expresses wt S6K1

Depositor

David Sabatini

Article

Kang et al Science. 2013 Jul 26;341(6144):1236566.

Insert

S6K1 (Rps6kb1 Rat)

Use

Lentiviral

Tags

FLAG and barcode: GGATCC

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2013

Availability

Academic Institutions and Nonprofits only
pLJM60 mUlk1 (wt) barcoded

Plasmid

#48799

Purpose

expresses wt Ulk1

Depositor

David Sabatini

Article

Kang et al Science. 2013 Jul 26;341(6144):1236566.

Insert

ULK1 (Ulk1 Mouse)

Use

Lentiviral

Tags

FLAG and barcode: GGATCC

Expression

Mammalian

Promoter

CMV

Available Since

May 2, 2014

Availability

Academic Institutions and Nonprofits only
AAV-FLIM-AKAR

Plasmid

#63058

Purpose

Expresses the Fluorescence Lifetime Imaging Microscopy (FLIM)-compatible PKA activity sensor FLIM-AKAR in an AAV backbone

Depositor

Bernardo Sabatini

Article

Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

Insert

FLIM-AKAR

Use

AAV

Expression

Mammalian

Promoter

EF-1α

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pMSCV-TAP mLST8 (aka: GbL)

Plasmid

#12575

Depositor

David Sabatini

Article

Frias et al Curr Biol. 2006 Sep 19;16(18):1865-70. d

Insert

mLST8 (MLST8 Human)

Use

Retroviral

Tags

TAP

Expression

Mammalian

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
pLJM60-S6K1(T389S) barcoded

Plasmid

#48800

Purpose

expresses mutant S6K1

Depositor

David Sabatini

Article

Kang et al Science. 2013 Jul 26;341(6144):1236566.

Insert

S6K1 T389S (Rps6kb1 Rat)

Use

Lentiviral

Tags

FLAG and barcode: GGTACC

Expression

Mammalian

Mutation

T389S

Promoter

CMV

Available Since

Dec. 18, 2013

Availability

Academic Institutions and Nonprofits only
AAV-FLEX-PKIalpha-IRES-nls-mRuby2

Plasmid

#63059

Purpose

Expresses PKIalpha and nuclear localized mRuby2 (linked with IRES) in a Cre-dependent manner in an AAV backbone

Depositor

Bernardo Sabatini

Article

Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

Insert

PKIalpha (Pkia Mouse, Synthetic)

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-tmeGFP-IRES-nls-mRuby2

Plasmid

#63060

Purpose

Expresses truncated monomeric eGFP and nuclear localized mRuby2 (linked with IRES) in a Cre-dependent manner in an AAV backbone

Depositor

Bernardo Sabatini

Article

Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

Insert

nls-mRuby2

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC6A1

Plasmid

#132242

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC6A1 (SLC6A1 Human)

Expression

Mammalian

Available Since

Nov. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC6A1_STOP

Plasmid

#161408

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC6A1 (SLC6A1 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pRK5 HA GST Rheb1

Plasmid

#14951

Depositor

David Sabatini

Article

Sancak et al Mol Cell. 2007 Mar 23. 25(6):903-15.

Insert

Rheb1 (RHEB Human)

Tags

HA-GST

Expression

Mammalian

Available Since

May 10, 2007

Availability

Academic Institutions and Nonprofits only

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scramble shRNA

Plasmid

#1864

Purpose

3rd gen lentiviral negative control vector containing scrambled shRNA

Depositor

David Sabatini

Article

Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

Insert

scramble

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

April 5, 2006

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-Opti

Plasmid

#163126

Purpose

LentiCRISPRv2 variant with modified sgRNA scaffold from Chen et al. 2013 Cell.

Depositor

David Sabatini

Article

Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pQCXI Puro DsRed-LC3-GFP

Plasmid

#31182

Depositor

David Sabatini

Article

Sheen et al Cancer Cell. 2011 May 17. 19(5):613-28.

Insert

DsRed-LC3-GFP

Use

Retroviral

Expression

Mammalian

Available Since

June 17, 2011

Availability

Academic Institutions and Nonprofits only

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pMXs-C3orf17-gfp

Plasmid

#70265

Purpose

Expresses C3orf17 in mammalian cells with GFP reporter.

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

C3orf17 (NEPRO Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

Nov. 9, 2015

Availability

Academic Institutions and Nonprofits only
pRK5 HA GST rap2a

Plasmid

#14952

Depositor

David Sabatini

Article

Sancak et al Mol Cell. 2007 Mar 23. 25(6):903-15.

Insert

rap2a (RAP2A Human)

Tags

HA-GST

Expression

Mammalian

Available Since

May 10, 2007

Availability

Academic Institutions and Nonprofits only
HA GST p85 S6K1 pRK5

Plasmid

#8466

Depositor

David Sabatini

Article

Ali et al J Biol Chem 2005 Apr 4;.

Insert

p85 s6k1 (Rps6kb1 Rat)

Tags

ha/gst

Expression

Mammalian

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only
pLJM60-Tiar

Plasmid

#38244

Depositor

David Sabatini

Article

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

Tiar (Tial1 Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2012

Availability

Academic Institutions and Nonprofits only
pMXs-C9orf114-gfp

Plasmid

#70266

Purpose

Expresses C9orf114 in mammalian cells with GFP reporter.

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

C9orf114 (SPOUT1 )

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

Nov. 9, 2015

Availability

Academic Institutions and Nonprofits only
pMXs-3XHA-EGFP-OMP25

Plasmid

#83356

Purpose

For tagging mitochondria with HA epitopes (HA-MITO construct)

Depositor

David Sabatini

Article

Chen et al Cell. 2016 Aug 25;166(5):1324-1337.e11.

Insert

3XHA-EGFP-OMP25

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

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HA Raptor

Plasmid

#8513

Depositor

David Sabatini

Article

Kim et al Cell 2002 Jul 26;110(2):163-75.

Insert

Raptor (RPTOR Human)

Tags

ha

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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