We narrowed to 11,059 results for: ena

1. pENAAV-cTNT-actn2-BioID2-HA

   Plasmid

   #216831

   Purpose

   cardiomyocytes-specific plasmid, expressing the coding sequence of mouse Actn2, biotin ligase BioID2, and a HA tag.

   Depositor

   Yuxuan Guo

   Article

   Guo et al Acta Pharmacol Sin. 2024 Jul 23. doi: 10

   Insert

   BioID-HA (Actn2 Synthetic)
   

   Use

   AAV

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   Aug. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV_3xgRNA;PTENA, p53B,SMAD4A_CAG_FlPO_synthPA

   Plasmid

   #68346

   Purpose

   Used to produce AAV expressing the FlpO recombinase and pig gRNA towards 3 tumor suppressors: PTEN, p53 and SMAD4

   Depositor

   Jannik Elverløv-Jakobsen

   Article

   Berthelsen et al Cancers (Basel). 2021 Jun 16;13(12). pii

   Inserts

   3xgRNA;PTENA, p53B,SMAD4A
   FlPO
   

   Use

   AAV; Flp/frt

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and U6

   Available since

   Dec. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-dCas13-NES-eNAT10

   Plasmid

   #238299

   Purpose

   Expresses cytoplasmic version of the programmable RNA acetylation system.

   Depositor

   Won Do Heo

   Article

   Yu et al Nat Chem Biol. 2025 Jun 2. doi: 10.1038/

   Insert

   dPspCas13b fused to eNAT10 (NAT10 Human, Prevotella sp. P5-125)
   

   Use

   CRISPR

   Tags

   HIV NES (C terminal of dPspCas13b)

   Expression

   Mammalian

   Mutation

   H133A for catalytically inactive mutant, deletion…

   Promoter

   Available since

   June 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNS49 (enAsCas12a-KRAB piggyBac)

   Plasmid

   #217335

   Purpose

   piggyBac expresion of enAsCas12a-KRAB

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   enAsCas12a
   

   Use

   CRISPR

   Tags

   6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

   Expression

   Mammalian

   Mutation

   E174R/S542R/K548R

   Promoter

   CAG

   Available since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. penaav-tnt-3xha-p2a-gfp

   Plasmid

   #175090

   Purpose

   For making AAV9

   Depositor

   William Pu

   Article

   Akerberg et al Nat Commun. 2019 Oct 28;10(1):4907. doi:

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. 3xNLS-NLP-cMyc-cMyc enAspCas12a

   Plasmid

   #182127

   Purpose

   pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc enAspCas12a in bacteria

   Depositor

   Scot Wolfe

   Article

   Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

   Insert

   3xNLS-NLP-cMyc-cMyc enAspCas12a
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-NLS-dCas13-NLS-eNAT10

   Plasmid

   #238300

   Purpose

   Expresses nuclear version of the programmable RNA acetylation system.

   Depositor

   Won Do Heo

   Article

   Yu et al Nat Chem Biol. 2025 Jun 2. doi: 10.1038/

   Insert

   dPspCas13b fused to eNAT10 (NAT10 Human, Prevotella sp. P5-125)
   

   Use

   CRISPR

   Tags

   SV40 BPNLS and SV40 BPNLS (C terminal of dPspCas1…

   Expression

   Mammalian

   Mutation

   H133A for catalytically inactive mutant, deletion…

   Promoter

   Available since

   June 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. 1N/2CxNLS-cMyc-NLP-cMyc enAspCas12a

   Plasmid

   #182126

   Purpose

   pET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc enAspCas12a in bacteria

   Depositor

   Scot Wolfe

   Article

   Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

   Insert

   1N/2CxNLS-cMyc-NLP-cMyc AspCas12aen
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. hum alpha ENAC promoter a25-a23

   Plasmid

   #83427

   Purpose

   promoter reporter construct to test 5' flanking sequences

   Depositor

   Christie Thomas

   Article

   Sayegh et al J Biol Chem. 1999 Apr 30;274(18):12431-7

   Insert

   human aENaC gene 5' flanking seq + 55 nt of the 5' UTR for exon 1A (SCNN1A Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mutation

   Promoter

   alpha ENAC

   Available since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pETduet-1:MCS1(HemeOxygenase)::MCS2(ReBphP-PCM)

    Plasmid

    #156463

    Purpose

    Express Heme Oxygenase (Nostocaceae) and ReBphP-PCM

    Depositor

    Andre Stiel

    Article

    Mishra et al Sci Adv. 2020 Jun 12;6(24):eaaz6293. doi

    Inserts

    Heme Oxygenase
    ReBphP-PCM
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAR010 Lenti pEF-dEnAsCas12a-NLS-3XHA-T2A-BSD

    Plasmid

    #195543

    Purpose

    dCas12a effector fusion for manipulating transcription

    Depositor

    Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Lenti pEF-dEnAsCas12a-NLS-3XHA-T2A-BSD
    

    Use

    CRISPR and Lentiviral

    Tags

    3XHA

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. AiO-Cas12a: All in one enAsCas12a expression and gRNA cloning backbone

    Plasmid

    #228364

    Purpose

    mU6-driven expression of enAsCas12a gRNAs with EF1a-enAsCas12a-2A-Puro

    Depositor

    Michael Boettcher

    Article

    El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral; Enascas12a nuclease and en…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-enAsCas12a(E174R/S542R/K548R)-NLS(nuc)-3xHA (AAS848)

    Plasmid

    #107941

    Purpose

    Mammalian expression plasmid for human codon optimized enAsCas12a (enhanced AsCas12a) encoding E174R/S542R/K548R substitutions

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized enAsCas12a (E174R/S542R/K548R)
    

    Use

    Tags

    3x HA and NLS (nucleoplasmin)

    Expression

    Mammalian

    Mutation

    E174R, S542R and K548R

    Promoter

    CAG

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAR014 Lenti pEF-dEnAsCas12a-NLS-VPR-3XHA-T2A-BSD

    Plasmid

    #195548

    Purpose

    dCas12a effector fusion for manipulating transcription

    Depositor

    Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Lenti pEF-dEnAsCas12a-NLS-VPR-3XHA-T2A-BSD
    

    Use

    CRISPR and Lentiviral

    Tags

    3XHA

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAR045 Lenti pEF-dEnAsCas12a-NLS-QNZF-3XHA-T2A-BSD

    Plasmid

    #195549

    Purpose

    dCas12a effector fusion for manipulating transcription

    Depositor

    Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Lenti pEF-dEnAsCas12a-NLS-QNZF-3XHA-T2A-BSD
    

    Use

    CRISPR and Lentiviral

    Tags

    3XHA

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAR013 Lenti pEF-dEnAsCas12a-NLS-VP64-3XHA-T2A-BSD

    Plasmid

    #195547

    Purpose

    dCas12a effector fusion for manipulating transcription

    Depositor

    Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Lenti pEF-dEnAsCas12a-NLS-VP64-3XHA-T2A-BSD
    

    Use

    CRISPR and Lentiviral

    Tags

    3XHA

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET-28b-T7-henAsCas12a(E174R/S542R/K548R)-NLS(nucleoplasmin)-6xHis (AAS1885)

    Plasmid

    #114072

    Purpose

    T7 promoter bacterial expression plasmid for human codon optimized enAsCas12a(E174R/S542R/K548R) with C-terminal NLS and His-tag

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized enAsCas12a (E174R/S542R/K548R) with C-terminal NLS and His-tag
    

    Use

    Tags

    NLS(nucleoplasmin) and 6xHis

    Expression

    Bacterial

    Mutation

    E174R, S542R, and K548R

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAR028 Lenti pEF-dEnAsCas12a-NLS-ZNF705F KRAB-3XHA-T2A-BSD

    Plasmid

    #195545

    Purpose

    dCas12a effector fusion for manipulating transcription

    Depositor

    Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Lenti pEF-dEnAsCas12a-NLS-TopKRAB-3XHA-T2A-BSD
    

    Use

    CRISPR and Lentiviral

    Tags

    3XHA

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAR012 Lenti pEF-dEnAsCas12a-NLS-ZNF10 KRAB-3XHA-T2A-BSD

    Plasmid

    #195550

    Purpose

    dCas12a effector fusion for manipulating transcription

    Depositor

    Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Lenti pEF-dEnAsCas12a-NLS-ZNF10 KRAB-3XHA-T2A-BSD
    

    Use

    CRISPR and Lentiviral

    Tags

    3XHA

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-T7Sequenase (JO584)

    Plasmid

    #208955

    Purpose

    A variant CE1 construct with T7 Sequenase DNA polymerase, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-Sequenase-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A)

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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