Addgene

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FGF10-luciferase

Plasmid

#24994

Depositor

Benoit Bruneau

Article

Agarwal et al Development. 2003 Feb . 130(3):623-33.

Insert

Fgf10 promoter (Fgf10 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2010

Availability

Academic Institutions and Nonprofits only
CAGGS-EGFP-NLS-3XFLAG-V5

Plasmid

#196090

Purpose

Expresses gfp in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag

Depositor

Mauro Calabrese

Article

Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

Insert

GFP

Use

Tags

C-terminal 3xFLAG and C-terminal V5

Expression

Bacterial and Mammalian

Mutation

Promoter

CAGGs

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CMV-N-HA-Pcdh15_mini_V4

Plasmid

#199185

Purpose

An AAV plasmid designed to facilitate the expression of mini-PCDH15 in the inner ear of a mouse.

Depositor

David Corey

Article

Ivanchenko et al Nat Commun. 2023 Apr 26;14(1):2400. doi:

Insert

HA-mmPcdh15_mini_V4-CD2

Use

AAV

Tags

Human influenza hemagglutinin (HA)

Expression

Mutation

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV_-CMV-Pcdh15_mini_V4-CD2

Plasmid

#199184

Purpose

An AAV plasmid designed to facilitate the expression of mini-PCDH15 in the inner ear of a mouse.

Depositor

David Corey

Article

Ivanchenko et al Nat Commun. 2023 Apr 26;14(1):2400. doi:

Insert

mmPcdh15_mini_V4-CD2

Use

AAV

Tags

Expression

Mutation

EC4, EC5, EC6, EC7, EC8 are deleted from msPCDH15…

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-Pcdh15_mini_V8-CD2

Plasmid

#199187

Purpose

An AAV plasmid designed to facilitate the expression of mini-PCDH15 in the inner ear of a mouse.

Depositor

David Corey

Article

Ivanchenko et al Nat Commun. 2023 Apr 26;14(1):2400. doi:

Insert

mmPcdh15_mini_V8-CD2

Use

AAV

Tags

Expression

Mutation

EC5, EC6, EC8, EC9, EC10 are deleted from msPCDH1…

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pb-miRE-tre-3xFLAG-hnRNPU

Plasmid

#196087

Purpose

Expresses mouse hnRNPU with a 3xFLAG tag in mammalian cells

Depositor

Mauro Calabrese

Article

Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

Insert

SAF-A (Hnrnpu Mouse)

Use

Tags

N-terminal 3xFLAG

Expression

Bacterial and Mammalian

Mutation

Promoter

TRE

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-Pcdh15_mini_V7-CD2

Plasmid

#199186

Purpose

An AAV plasmid designed to facilitate the expression of mini-PCDH15 in the inner ear of a mouse.

Depositor

David Corey

Article

Ivanchenko et al Nat Commun. 2023 Apr 26;14(1):2400. doi:

Insert

mmPcdh15_mini_V7-CD2

Use

AAV

Tags

Expression

Mammalian

Mutation

EC4, EC5, EC6, EC9, EC10 are deleted from msPCDH1…

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pb-miRE-tre-3xFLAG-hnRNPU∆RGG

Plasmid

#196088

Purpose

Expresses mouse hnRNPU lacking RGG domain with a 3xFLAG tag in mammalian cells

Depositor

Mauro Calabrese

Article

Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

Insert

SAF-A (Hnrnpu Mouse)

Use

Tags

N-terminal 3xFLAG

Expression

Bacterial and Mammalian

Mutation

removed last 154 amino acids on cterminal end

Promoter

TRE

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pSicoR (Puro) p65 shRNA1

Plasmid

#22507

Depositor

Tyler Jacks

Article

Meylan et al Nature. 2009 Nov 5;462(7269):104-7. doi:

Insert

p65 shRNA1 (Rela Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 5, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pSicoR (Puro) p65 shRNA2

Plasmid

#22508

Depositor

Tyler Jacks

Article

Meylan et al Nature. 2009 Nov 5;462(7269):104-7. doi:

Insert

p65 shRNA2 (Rela Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2010

Availability

Academic Institutions and Nonprofits only
CAGGS-MCS-NLS-3XFLAG-V5

Plasmid

#196383

Purpose

Empty backbone vector to insert cDNA for strong expression in mammalian cells

Depositor

Mauro Calabrese

Article

Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

Type

Empty backbone

Use

Tags

3xFLAG and V5

Expression

Bacterial and Mammalian

Mutation

Promoter

CAGGs

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
K15-EGFP

Plasmid

#44266

Depositor

George Cotsarelis

Article

Morris et al Nat Biotechnol. 2004 Apr;22(4):411-7. Ep

Insert

K15 promoter (-4.8) (Krt15 Mouse)

Use

Mouse Targeting; Fluorescence microscopy

Tags

EGFP

Expression

Mammalian

Mutation

Contains the murine K15 promoter (-4.8) fragment

Promoter

murine K15 promoter (-4.8) fragment

Available since

April 2, 2013

Availability

Academic Institutions and Nonprofits only
pSynapsin_NLS_GFP_P2A_Halo_GluA2

Plasmid

#170454

Purpose

Expresses halo tagged GluA2 in neuronal cells

Depositor

Shigeki Watanabe

Article

Li et al Nat Commun. 2021 Jan 29;12(1):677. doi:

Insert

GluA2 (Gria2 Mouse)

Use

Lentiviral

Tags

Halo

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSynapsin_NLS_GFP_P2A_SNAP_NR1

Plasmid

#170458

Purpose

Expresses SNAP tagged NR1 in neuronal cells

Depositor

Shigeki Watanabe

Article

Li et al Nat Commun. 2021 Jan 29;12(1):677. doi:

Insert

NR1 (Grin1 Rat)

Use

Lentiviral

Tags

SNAP

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSynapsin_NLS_GFP_P2A_His6_NR1.

Plasmid

#170457

Purpose

Expresses histidine tagged NR1 in neuronal cells

Depositor

Shigeki Watanabe

Article

Li et al Nat Commun. 2021 Jan 29;12(1):677. doi:

Insert

NR1 (Grin1 Rat)

Use

Lentiviral

Tags

Hexa-histidin

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSynapsin_NLS_GFP_P2A_His6_GluA2

Plasmid

#170453

Purpose

Expresses histidine tagged GluA2 in neuronal cells

Depositor

Shigeki Watanabe

Article

Li et al Nat Commun. 2021 Jan 29;12(1):677. doi:

Insert

GluA2 (Gria2 Mouse)

Use

Lentiviral

Tags

Hexa-histidine

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSynapsin_NLS_GFP-P2A_SNAP_GluA2

Plasmid

#170455

Purpose

Expresses SNAP tagged GluA2 in neuronal cells

Depositor

Shigeki Watanabe

Article

Li et al Nat Commun. 2021 Jan 29;12(1):677. doi:

Insert

GluA2 (Gria2 Mouse)

Use

Lentiviral

Tags

SNAP

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5/FRT-mMAPT

Plasmid

#231584

Purpose

Minigene of mouse MAPT containing exon 9, 10, 11 and relevant intronic sequences

Depositor

Chaolin Zhang

Article

Recinos et al Cell Genom. 2024 Jun 12;4(6):100563. doi

Insert

mouse MAPT (Mapt Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHiUGE TurboID-HA donor (all-in-one) ORF0

Plasmid

#200383

Purpose

Tagging endogenously expressed proteins with TurboID-HA, C-terminal

Depositor

Scott Soderling

Article

Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

Insert

TurboID (ref: PMID: 30125270, Addgene plasmid # 107169)

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHiUGE TurboID-HA donor (all-in-one) ORF2

Plasmid

#200385

Purpose

Tagging endogenously expressed proteins with TurboID-HA, C-terminal

Depositor

Scott Soderling

Article

Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

Insert

TurboID (ref: PMID: 30125270, Addgene plasmid # 107169)

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge