We narrowed to 529 results for: ppat

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pET28a-Ec.coaD (pESC106)

Plasmid

#50388

Purpose

Heterologous expression of E. coli CoA biosynthetic enzyme, PPAT (phosphopantetheine adenylyltransferase). Contain 6xHis-tag for nickel affinity purification.

Depositor

Tadhg Begley, Erick Strauss

Article

Strauss et al J Biol Chem. 2002 Dec 13;277(50):48205-9

Insert

PPAT

Tags

6xHis-tag

Expression

Bacterial

Promoter

T7

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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pJK683

Plasmid

#72378

Purpose

Produces human phosphoribosyl pyrophosphate amidotransferase (PRAT), synthetic gene

Depositor

T. J. Kappock

Insert

phosphoribosyl pyrophosphate amidotransferase (PPAT Human)

Expression

Bacterial

Available Since

March 4, 2016

Availability

Academic Institutions and Nonprofits only

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pJK678

Plasmid

#72376

Purpose

Produces human phosphoribosyl pyrophosphate amidotransferase (PRAT), synthetic gene

Depositor

T. J. Kappock

Insert

phosphoribosyl pyrophosphate amidotransferase (PPAT Human)

Use

Gene synthesis

Promoter

n/a

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pJK694

Plasmid

#72379

Purpose

Produces human phosphoribosyl pyrophosphate amidotransferase (PRAT), synthetic gene

Depositor

T. J. Kappock

Insert

phosphoribosyl pyrophosphate amidotransferase (PPAT Human)

Expression

Yeast

Promoter

GPD

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only
EF2451_pET21a(+)

Plasmid

#13840

Depositor

SeWon Suh

Article

Kang et al Acta Crystallograph Sect F Struct Biol C

Insert

PPAT

Tags

His

Expression

Bacterial

Available Since

June 29, 2007

Availability

Academic Institutions and Nonprofits only

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pQTEV-COASY

Plasmid

#34816

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

Coenzyme A synthase (COASY Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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COASY gRNA (BRDN0001145497)

Plasmid

#75818

Purpose

3rd generation lentiviral gRNA plasmid targeting human COASY

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

COASY (COASY Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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COASY gRNA (BRDN0001145628)

Plasmid

#75819

Purpose

3rd generation lentiviral gRNA plasmid targeting human COASY

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

COASY (COASY Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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COASY gRNA (BRDN0001147007)

Plasmid

#75820

Purpose

3rd generation lentiviral gRNA plasmid targeting human COASY

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

COASY (COASY Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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COASY gRNA (BRDN0001147178)

Plasmid

#75821

Purpose

3rd generation lentiviral gRNA plasmid targeting human COASY

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

COASY (COASY Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLEXSY-hyg2.1_LtIFT43_Flag

Plasmid

#194433

Purpose

Expression of a flag tagged version of L. tarentolae intraflagellar transport protein-43 (IFT43) in Leishmania tarentolae

Depositor

Alan Brown

Article

Meleppattu et al Cell. 2022 Dec 22;185(26):4986-4998.e12.

Insert

Intraflagellar transport complex subunit 43

Use

Leishmania tarentolae expression

Tags

3X Flag and Linker

Promoter

L.donovani promoter elements

Available Since

June 2, 2023

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF1D06

Plasmid

#70813

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

kappaTry (kappaTry Fly)

Use

Gateway entry vector

Available Since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
EGFR WT

Plasmid

#11011

Purpose

Retroviral construct for expressing human EGFR in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR (EGFR Human)

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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EGFR L858R

Plasmid

#11012

Purpose

Retroviral construct for expressing human EGFR with L858R mutation in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR L858R (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

L858R (tumor-derived)

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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EGFR D837A

Plasmid

#11014

Purpose

Retroviral construct for expressing human EGFR with D837A mutation in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR D837A (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

D837A

Available Since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only
EGFR G719S

Plasmid

#11013

Purpose

Retroviral construct for expressing human EGFR with G719S mutation in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR G719S (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

G719S

Available Since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only
EGFR D770-N771 insNPG

Plasmid

#11016

Purpose

Retroviral construct for expressing human EGFR with D770-N771 insNPG mutation in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR D770-N771 insNPG (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

D770-N771 insNPG

Available Since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only
EGFR (del3) L747-E749del, A750P

Plasmid

#11015

Purpose

Retroviral construct for expressing human EGFR with L747-E749 deletion and A750P mutation in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR (del3) (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

L747-E749 deleted, A750P

Available Since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only
px552-U6-gRNA1-U6-gRNA2-CMV-eGFP

Plasmid

#211760

Purpose

Paired gRNAs (sgRNA1 and 2) targeting Exon 1 of VEGFA gene conserved across mouse, rhesus macaque, and human.

Depositor

Glenn Yiu

Article

Chung et al Mol Ther Nucleic Acids. 2022 Apr 27;28:6

Insert

gRNA1 and gRNA2 targeting VEGF-A (VEGFA Mouse, M. mulatta (rhesus macaque), Human)

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 6, 2024

Availability

Academic Institutions and Nonprofits only

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px552-U6-gRNA1-CMV-eGFP

Plasmid

#211758

Purpose

sgRNA1 targeting Exon 1 of VEGFA gene conserved across mouse, rhesus macaque, and human.

Depositor

Glenn Yiu

Article

Chung et al Mol Ther Nucleic Acids. 2022 Apr 27;28:6

Insert

gRNA1 targeting VEGF-A (VEGFA Mouse, M. mulatta (rhesus macaque), Human)

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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