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SRPK3 gRNA (BRDN0001146524)

Plasmid

#77395

Purpose

3rd generation lentiviral gRNA plasmid targeting human SRPK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SRPK3 (SRPK3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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RPS6KA5 gRNA (BRDN0001146211)

Plasmid

#77371

Purpose

3rd generation lentiviral gRNA plasmid targeting human RPS6KA5

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RPS6KA5 (RPS6KA5 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pEF6-CSPG4-myc-his

Plasmid

#69037

Purpose

Expression of CSPG4 in mammalian cells

Depositor

Wensheng Wei

Article

Yuan et al Cell Res. 2015 Feb;25(2):157-68. doi: 10

Insert

CSPG4 (CSPG4 Human)

Use

Tags

His and Myc

Expression

Mammalian

Mutation

none

Promoter

EF-1 alpha

Available since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only

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Integrin Beta1-mPA-GFP

Plasmid

#186302

Purpose

Expresses mPA-GFP tagged Integrin beta-1 in mammalian cells on a CMV promoter

Depositor

Pakorn Kanchanawong

Article

Jain et al Nat Protoc. 2022 Jul 27. pii: 10.1038/s4

Insert

Integrin Beta 1 (ITGB1 Human)

Use

Tags

mPA-GFP

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR2b-ITGB1(WT)

Plasmid

#215450

Purpose

Gateway entry vector with integrin beta1 wild-type (WT)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

ITGB1 (ITGB1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

Silent mutations added to disrupt shRNA binding a…

Promoter

none

Available since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pPB.DEST-ITGB1(WT)-mRuby2

Plasmid

#215448

Purpose

Piggybac expression vector with integrin beta1 tagged with mRuby2

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

ITGB1 (ITGB1 Human)

Use

Transposon-based stable expression

Tags

mRuby2

Expression

Mammalian

Mutation

Silent mutations added to disrupt shRNA binding a…

Promoter

CAG

Available since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-BUBR1

Plasmid

#234704

Purpose

expression of fusion protein

Depositor

Lienhard Schmitz

Article

Seibert et al J Cell Biol. 2019 Apr 1;218(4):1164-1181

Insert

BUBR1 (BUB1B Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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PD2529 human beta1 ecto

Plasmid

#221398

Purpose

Mammalian expression of human integrin beta1 ectodomain

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 ectodomain (ITGB1 Human)

Use

Tags

AVI tag, HRV3C cleavage site, basic coil, HA tag,…

Expression

Mammalian

Mutation

codon optimized for human mature _1 residues Q1 t…

Promoter

Available since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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Beta1 Q280* K418* full-length

Plasmid

#221406

Purpose

Mammalian expression of human integrin beta1 Q280* K418* full-length

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 Q280* K418* full-length (ITGB1 Human)

Use

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …

Expression

Mammalian

Mutation

codon-optimized mature sequence

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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Beta1 N391* N562* full-length

Plasmid

#221407

Purpose

Mammalian expression of human integrin beta1 N391* N562* full-length

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 N391* N562* full-length (ITGB1 Human)

Use

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …

Expression

Mammalian

Mutation

codon-optimized mature sequence

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge