We narrowed to 14,179 results for: NTS

1. pET28a-FIP3

   Plasmid

   #74753

   Purpose

   Expressed sfGFP-tagged FIP3 in E. coli for protein production

   Depositor

   Ron Vale

   Article

   Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

   Insert

   Rab11 family interacting protein 3 (RAB11FIP3 Human)
   

   Use

   Tags

   6x His, Strep II, and superfolder GFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBW842_pCAG-pExpr-FP-DECODER

   Plasmid

   #87551

   Purpose

   Expresses BFP, GFP, iRFP, and mRuby in response to combinations of Cre and Flp recombinases

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   mtagBFP, EGFP, iRFP720, mRuby2
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGL3-LRF

   Plasmid

   #66936

   Purpose

   Destination vector; gateway cassette cloned upstream of basal promoter driving luciferase. Forward orientation (attR1, attR2).

   Depositor

   Alvaro Monteiro

   Article

   Buckley et al Nat Protoc. 2016 Jan;11(1):46-60. doi: 1

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV-40

   Available since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pCRL1

   Plasmid

   #153997

   Purpose

   sgRNA in vitro transcription plasmid.

   Depositor

   Shengdar Tsai

   Article

   Lazzarotto et al Nat Protoc. 2018 Nov;13(11):2615-2642. d

   Type

   Empty backbone

   Use

   CRISPR; Sgrna under a t7 promoter for in vitro tr…

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   June 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRPL28(K55M) SpHIS5 (pNTI666)

   Plasmid

   #115436

   Purpose

   Expresses K55M RPL28 from HIS3 yeast plasmid

   Depositor

   Nicholas Ingolia

   Article

   McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

   Inserts

   RPL28 (RPL28 Budding Yeast)
   HIS5
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   K55M; MfeI site in intron

   Promoter

   Ashbya gospii TEF1 and endogenous

   Available since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCIS-krPAM2

   Plasmid

   #25457

   Depositor

   Richard Mains

   Article

   Milgram et al J Cell Biol. 1992 May . 117(4):717-28.

   Insert

   PAM2 (Pam Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Exon A deleted (exon A extends from nt 1475 to n…

   Promoter

   Available since

   July 13, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pGL3-LRR

   Plasmid

   #66937

   Purpose

   Destination vector; gateway cassette cloned upstream of basal promoter driving luciferase. Reverse orientation (attR2, attR1).

   Depositor

   Alvaro Monteiro

   Article

   Buckley et al Nat Protoc. 2016 Jan;11(1):46-60. doi: 1

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV-40

   Available since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pBW808_pCAG-PV5-VloxP-loxN-F3-GFP-BGHpA

   Plasmid

   #87584

   Purpose

   PV5 for 3-input, 2-output Full Adder

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   EGFP
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAM5089

   Plasmid

   #85125

   Purpose

   KaiB expressed from native promoter from NSII-Nt. Complements rhythms of kaiB null strain.

   Depositor

   Susan Golden

   Article

   Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d

   Insert

   PkaiB-kaiB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBW829_pCAG-pExpr-LUT

    Plasmid

    #87554

    Purpose

    Encodes Boolean Look-up Table logic in response to Cre, Flp, PhiC31, Vika, B3, and Bxb1 recombinases

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    EGFP
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBW2287_pCAG-PV1-PYL-L1-FlpO-397C-NLS-BGHpA

    Plasmid

    #87560

    Purpose

    Expresses aa397-423 of Flp recombinase fused to PYL CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    FlpO aa397-423
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    PYL

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBW2286_pCAG-PV1-FlpO-N396-L1-ABI-NLS-BGHpA

    Plasmid

    #87559

    Purpose

    Expresses aa1-396 of Flp recombinase fused to ABI CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    FlpO aa1-396
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    ABI

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX6P1-C. thermophilum LIC

    Plasmid

    #74780

    Purpose

    encodes GST-tagged dynein light intermediate chain from Chaetomium thermophilum

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    dynein light intermediate chain (Chaetomium thermophilum)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBW811_pCAG-PV8-F14-GFP-T2A-mCherry-BGHpA

    Plasmid

    #87587

    Purpose

    PV8 for 3-input, 2-output Full Adder

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    EGFP, mCherry
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pYO411

    Plasmid

    #235732

    Purpose

    Protein expression of ZZ-FL yeast Atg14 in yeast

    Depositor

    Roger Williams

    Article

    Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

    Insert

    ATG14
    

    Use

    Tags

    3xTEV-ZZ

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pYO69

    Plasmid

    #235747

    Purpose

    Protein expression of FL ScVPS34 in yeast

    Depositor

    Roger Williams

    Article

    Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

    Insert

    VPS34
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    S2A

    Promoter

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pYO361

    Plasmid

    #235748

    Purpose

    Protein expression of ScVPS34 HELCAT

    Depositor

    Roger Williams

    Article

    Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

    Insert

    VPS34 HELCAT
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    aa 268-875

    Promoter

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pYO227

    Plasmid

    #235749

    Purpose

    Protein expression of ZZ-FL ScVPS30

    Depositor

    Roger Williams

    Article

    Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

    Insert

    VPS30
    

    Use

    Tags

    ZZ-3xTEV

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pYO832

    Plasmid

    #235755

    Purpose

    Protein expression of ScVPS30 (1-215E), untagged

    Depositor

    Roger Williams

    Article

    Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

    Insert

    ScVPS30
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    aa 1-215E

    Promoter

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28b-M2

    Plasmid

    #225735

    Purpose

    To express M2 (219-399) in E. coli

    Depositor

    Nicole Sampson

    Article

    Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

    Insert

    mce3R
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 219-399

    Promoter

    Available since

    Jan. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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