We narrowed to 2,701 results for: LRE
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Plasmid#207607PurposesgRNA for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locusDepositorInsertcgactcgcccggcagcgcac
TagsNoneExpressionMammalianPromoterCMV and U6Available SinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
3xMS2 TR knockin sgRNA 2
Plasmid#207608PurposesgRNA 2 for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locusDepositorInsertacccccaaacctgactgact
TagsNoneExpressionMammalianPromoterCMV and U6Available SinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
TR knockout HRD
Plasmid#207579PurposeHomologous recombination donor replacing TR with a puro cassette.DepositorInsertpuro resistance cassette flanked by TR locus sequences excluding TR
TagsNoneExpressionMammalianPromoterSV40Available SinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF823_EFS-Cas9-P2A-Puro
Plasmid#211645PurposeEFS-Cas9-P2A-PuroDepositorInsertCas9
UseCRISPR and LentiviralExpressionMammalianAvailable SinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF226_EFS-Cas9-P2A-Puro
Plasmid#211644PurposeEFS-Cas9-P2A-PuroDepositorInsertCas9
UseCRISPR and LentiviralExpressionMammalianAvailable SinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLipoP1-NLS-3xmCitrine
Plasmid#192522PurposeFluorescent reporter for plant responses to bacterial infectionDepositorInsertPutative membrane lipoprotein (AT3G18250 Mustard Weed)
Tags3xmCitrine and NLSExpressionPlantPromoterLipoP1Available SinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-ASAP3ER
Plasmid#195358PurposeASAP3ER under control of human synapsin promoterDepositorInsertASAP3ER
UseAAVTagsGFPExpressionMammalianPromoterhuman synapsinAvailable SinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-Cas9-P2A-Puro_ST-sgRNA
Plasmid#188705PurposeLentivirus transfer plasmid encoding Cas9, puromycin resistance, and 4 sgRNAs targeting human safe targeting lociDepositorInsertST sgRNAs
UseLentiviralExpressionMammalianPromoterhUbCAvailable SinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-fDIO-tdTomato-WPRE
Plasmid#187112PurposeFLP-dependent expression of tdTomato under EF1a promoterDepositorInserttdTomato
UseAAVAvailable SinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_hNKD2
Plasmid#174329Purposeencodes human NKD2 cDNA with a C-terminal MycHis Tag.DepositorInsertHomo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
TagsMycHisExpressionMammalianPromoterCMVAvailable SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVD2
Plasmid#165857Purposeuniversal Vector Domesticator vector v2 used in the integration (domestication) of new vector backbone parts in the GB2.0 cloning system. Uses blue-white screening methodDepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGal4BD 1-147
Plasmid#165819Purpose1-147 AA of GAL4 DNA binding domain, requires assembly with TL2-TL4 linker and TL5 activation domainDepositorInsertGal4BD 1-147
ExpressionInsectAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGAL4AD 768-881
Plasmid#165820Purpose768-881 AA of GAL4 activation domain, requires assembly with TL2-TL4 linker and TL1 DNA binding domainDepositorInsertGAL4AD 768-881
ExpressionInsectAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
Promoterless TP10
Plasmid#165787PurposeDrosphila TP10 poly(a) terminator sequence in promoter grammar, for assembly of no expression control vectorsDepositorInsertPromoterless T10
ExpressionInsectAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
Bax gRNA#1
Plasmid#171827PurposeCas9-mediated knockout of Bax in mammalian cellsDepositorAvailable SinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
Bax gRNA#2
Plasmid#171828PurposeCas9-mediated knockout of Bax in mammalian cellsDepositorAvailable SinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
Bax gRNA#3
Plasmid#171829PurposeCas9-mediated knockout of Bax in mammalian cellsDepositorAvailable SinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAFNF-Cdc42
Plasmid#163606PurposeExpresses Cdc42 Flp-dependently in mammalian cellsDepositorInsertCdc42 (Cdc42 Mouse)
ExpressionMammalianAvailable SinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTub-alpha-1-mtGFP
Plasmid#138981PurposeNeuron-specific expression of membrane targeted EGFPDepositorInsertEGFP
ExpressionMammalianMutationEGFP (added membrane targeting domain from GAP43).Available SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGMR-WhiteA2
Plasmid#165874PurposeBasic transgenesis 5xGMR white CDS physical eye marker cassette in Alpha2, lacks attB siteDepositorInsertEye Marker Cassette
UseSynthetic BiologyAvailable SinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJJF496_dpy-10_CDS_sg6
Plasmid#164267PurposesgRNA targeting dpy-10 (CDS) in C. elegans. Backbone: pJJR50.DepositorInsertsgRNA targeting the coding sequence of dpy-10
UseCRISPRExpressionWormPromoterR07E5.16 (U6)Available SinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJJF495_dpy-10_CDS_sg6
Plasmid#164268PurposesgRNA targeting dpy-10 (CDS) in C. elegans. Backbone: pJJF439.DepositorInsertsgRNA targeting the coding sequence of dpy-10
UseCRISPRExpressionWormPromoterU6 promoter from W05B2.8Available SinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJJF449_dpy-10_CDS_sg1
Plasmid#163866PurposesgRNA targeting dpy-10 (CDS) in C. elegans. Backbone: pJJR50.DepositorInsertsgRNA targeting the coding sequence of dpy-10
UseCRISPRExpressionWormPromoterR07E5.16 (U6)Available SinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJJF328_sqt-3_3′UTR_sg2
Plasmid#163867PurposesgRNA targeting sqt-3 (3′UTR) in C. elegans. Backbone: pJJR50.DepositorInsertsgRNA targeting the 3'UTR of sqt-3
UseCRISPRExpressionWormPromoterR07E5.16 (U6)Available SinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJJF152_Phsp_Cas9
Plasmid#163862Purposeheat shock-induced expression of Cas9 in C. elegansDepositorInsertCas9, C. elegans codon optimized with intron and SV40_NLS
UseCRISPRExpressionWormPromoterPhsp-16.48Available SinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJJF143_SECGFP_sg1
Plasmid#163864PurposesgRNA targeting GFP in C. elegans. Backbone: pJJR50.DepositorInsertsgRNA targeting GFP
UseCRISPRExpressionWormPromoterR07E5.16 (U6)Available SinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHSP70B-CP6-ble
Plasmid#165880PurposeBasic Phleomycin resistance cassette in Alpha1, lacks physical marker or attB siteDepositorInsertBasic Selection Vector
UseSynthetic BiologyAvailable SinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-CDKL1_IDG-K
Plasmid#158176PurposeGateway destination clone of CDKL1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-CDKL1 (CDKL1 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianPromoterUbiquitinAvailable SinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC_h7SK M15 (CUA)
Plasmid#154784Purposeplasmid with single copy of Mma PylT M15 mutant (CUA anticodon)DepositorInsertMma PylT
ExpressionMammalianMutationG10A, G14U, G16U, C20U, C52APromoterh7SKAvailable SinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_M42FDHFR_WTTS
Plasmid#91237Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
Tags6xHisExpressionBacterialMutationM42FPromoterT7Available SinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_M42FG121VDHFR_R166QTS
Plasmid#91238Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
Tags6xHisExpressionBacterialMutationM42FG121 and R166QPromoterT7Available SinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_M42FG121VDHFR_WTTS
Plasmid#91239Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
Tags6xHisExpressionBacterialMutationM42FG121VPromoterT7Available SinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_W22HDHFR_R166QTS
Plasmid#91240Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
Tags6xHisExpressionBacterialMutationR166Q and W22HPromoterT7Available SinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_W22HDHFR_WTTS
Plasmid#91241Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
Tags6xHisExpressionBacterialMutationW22HPromoterT7Available SinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_WTEcoliDHFR_R166QTS
Plasmid#91242Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
Tags6xHisExpressionBacterialMutationR166QTSPromoterT7Available SinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_D27NDHFR_R166QTS
Plasmid#91243Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
Tags6xHisExpressionBacterialMutationD27N and R166QPromoterT7Available SinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_F31VDHFR_R166QTS
Plasmid#91245Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
Tags6xHisExpressionBacterialMutationF31V and R166QPromoterT7Available SinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_F31VDHFR_WTTS
Plasmid#91227Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
Tags6xHisExpressionBacterialMutationF31VPromoterT7Available SinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_F31YG121VDHFR_R166QTS
Plasmid#91228Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
Tags6xHisExpressionBacterialMutationF31YG121 and R166QPromoterT7Available SinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_F31YL54IDHFR_R166QTS
Plasmid#91230Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
Tags6xHisExpressionBacterialMutationF31YL54I and R166QPromoterT7Available SinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only