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Showing: 951 - 1000 of 6170 results

  12. pCDH-CMV4

    Plasmid
    #72284
    Purpose
    Express gene of interest under CMV promoter. WPRE has been removed to increase the cloning capacity.
    Depositor
    Kazuhiro Oka
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    Jan. 25, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72284-plasmid-map-sequence-id-310586

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  17. pREPROII-attB

    Plasmid
    #86557
    Purpose
    Dox inducible expression of reprogramming factors (Klf4, Sox2, Oct4, & cMyc), integration via phiC31 attB sites, excision via Cre/loxP
    Depositor
    Ralf Kuehn
    Article
    Merkl et al PLoS One. 2013;8(1):e55170. doi: 10.1371
    Inserts
    Oct4 (Pou5f1 Mouse)
    cMyc (Myc Mouse)
    Sox2 (Sox2 Mouse)
    Kruppel-like factor 4 (Klf4 Mouse)
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    86557-plasmid-map-sequence-id-168723

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  23. pLL3.7

    Plasmid
    #11795
    Purpose
    3rd generation lentiviral vector that expresses shRNA under the mouse U6 promoter. A CMV-EGFP reporter cassette is included in the vector to monitor expression.
    Depositor
    Luk Parijs
    Article
    Rubinson et al Nat Genet. 2003 Mar . 33(3):401-6.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Cre/Lox, Lentiviral, and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    mouse U6
    Available since
    July 19, 2006
    Availability
    Academic Institutions and Nonprofits only
    11795-plasmid-map-sequence-id-237575

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  33. pJ151-HDR

    Plasmid
    #113631
    Purpose
    Contains two recombination homology arms and loxP-flanked cassette encoding puromycin resistance gene, Venus fluorescence marker, thymidine kinase suicide gene, for scarless chromosomal engineering.
    Depositor
    Alex Kentsis
    Inserts
    Left homology arm
    Puromycin resistance gene
    Venus fluorescence gene
    Thymidine kinase
    Right homology arm
    Use
    Cre/Lox; Homology recombination selection vector
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    EF1a promoter, EF1a promoter (T2A cleaving sequen…
    Available since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    113631-plasmid-map-sequence-id-222216

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  34. pLP-hSox2-ires-gfp

    Plasmid
    #49391
    Purpose
    mammalian bicistronic expression of human Sox2 and EGFP
    Depositor
    Randall McKinnon
    Insert
    Sox2 (SOX2 Human)
    Use
    Cre/Lox; Acceptor vector
    Tags
    6xHN, EGFP, and IRES
    Expression
    Mammalian
    Mutation
    TGA (stop) to CCA (Pro) at nt 1027 with respect t…
    Promoter
    CMV
    Available since
    Dec. 2, 2013
    Availability
    Academic Institutions and Nonprofits only

  36. pLPS-hAkt1-gfp

    Plasmid
    #49388
    Purpose
    mammalian expression of human Akt1 fused to EGFP
    Depositor
    Randall McKinnon
    Insert
    Akt1 (AKT1 Human)
    Use
    Cre/Lox; Acceptor vector
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    tga to cca at nt 1725 with respect to GenBank ID …
    Promoter
    CMV
    Available since
    Nov. 21, 2013
    Availability
    Academic Institutions and Nonprofits only

  39. AbVec2.0-mIghg3

    Plasmid
    #127160
    Purpose
    Expression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG3 isotype
    Depositor
    Hedda Wardemann
    Insert
    Ighg3 (Ighg3 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hCMV IE1 gene promoter
    Available since
    Nov. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    127160-plasmid-map-sequence-id-256904

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  40. pFastBac1-melHis10TEV

    Plasmid
    #159428
    Purpose
    empty pFastBac1 secretion vector (polyhedrin promoter) adds N-term melittin signal sequence & TEV-protease-removable His10; avoids unwanted residues in final protein by using BseRI restriction sites
    Depositor
    Debra Hansen
    Type
    Empty backbone
    Use
    Tags
    melittin signal sequence (removed in vivo by sign…
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    159428-plasmid-map-sequence-id-333931

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  41. pXPR_070

    Plasmid
    #164265
    Purpose
    Lentiviral vector that enables Cre-mediated sgRNA expression. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.
    Depositor
    Arlene Sharpe
    Article
    LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s
    Insert
    sgRNA cassette
    Use
    CRISPR, Cre/Lox, and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human U6
    Available since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    164265-plasmid-map-sequence-id-330127

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  42. pAAV-Syn1-FLEX-mCh-T2A-FLAG-hMOR-WPRE

    Plasmid
    #166970
    Purpose
    Cre-dependent viral expression of the human mu opioid receptor (hMOR/Oprm1) and mCherry. hMOR contains an N-terminal FLAG tag.
    Depositor
    Matthew Banghart
    Article
    Liu et al Proc Natl Acad Sci U S A. 2021 Jun 8;118
    Insert
    Homo sapiens opioid receptor mu 1 (OPRM1) (OPRM1 Human)
    Use
    AAV and Cre/Lox
    Tags
    FLAG, T2A, and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    hsyn
    Available since
    Nov. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    166970-plasmid-map-sequence-id-346867

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  45. AbVec2.0-mIghg2c

    Plasmid
    #127159
    Purpose
    Expression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG2c isotype
    Depositor
    Hedda Wardemann
    Insert
    Ighg2c (Ighg2c Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hCMV IE1 gene promoter
    Available since
    Nov. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    127159-plasmid-map-sequence-id-256903

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  48. AbVec2.0-mIghg1

    Plasmid
    #127158
    Purpose
    Expression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG1 isotype
    Depositor
    Hedda Wardemann
    Insert
    Ighg1 (Ighg1 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hCMV IE1 gene promoter
    Available since
    Nov. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    127158-plasmid-map-sequence-id-256842

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  50. pET28GSTlinker-LIC

    Plasmid
    #119970
    Purpose
    Structure determination and a long linker aiming to keep GST tag free in DNA binding screening experiments.
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 9, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    119970-plasmid-map-sequence-id-232695

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Showing: 951 - 1000 of 6170 results