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Plasmid#192455PurposeExpresses BALF5 with D755N and D757N mutationsDepositorInsertBALF5 (D755N&D757N) (BALF5 Epstein-Barr Virus)
UseTagsFLAGExpressionMammalianMutationD755N & D757NPromoterAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
IRE-miR21mutant-SunTag-TREAT
Plasmid#196933PurposeSunTag cassette was added in the coding region in the miR21mutant -IRE-TREAT reporter.DepositorInsertRenilla luciferase
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
IRE-let7-SunTag-TREAT
Plasmid#196926PurposeSunTag cassette was added in the coding region in the let7-IRE-TREAT reporterDepositorInsertRenilla luciferase
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
IRE-let7R-SunTag-TREAT
Plasmid#196927PurposeSunTag cassette was added in the coding region in the let7R-IRE-TREAT reporter.DepositorInsertRenilla luciferase
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGTQL1221YC
Plasmid#61705PurposeGateway compatible BiFC vector (pEarleyGate202-YC)DepositorTypeEmpty backboneUseBifc assayTagsYFP-C and FLAGExpressionPlantMutationPromoter35SAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSCM167.2-Venus-VF-hADAM10(aa1-213)
Plasmid#189554PurposeProtein expression ADAM10 in mammalian cells. Contains only prodomain--amino acids 1-213DepositorInserttruncated ADAM metallopeptidase domain 10 (ADAM10 Human)
UseTagsVFExpressionMammalianMutationcontains aa 1-213PromoterAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
IRE-SunTag
Plasmid#196920PurposeThe 5UTR of the ferritin heavy chain mRNA was cloned upstream of the SunTag-Renilla coding sequence in the ΔIRE-SunTag reporterDepositorInsertRenilla luciferase
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
Let7-SunTag
Plasmid#196928PurposeLet7-miRNA binding sites (3x) were integrated in between the stop codon and MS2 stem-loops in the SunTag reporter.DepositorInsertRenilla luciferase
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
Let7R-SunTag
Plasmid#196929PurposeReverse complement of let7-miRNA binding sites (3x) were integrated in between the stop codon and MS2 stem-loops in the SunTag reporter.DepositorInsertRenilla luciferase
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
miR21mutant-IRE-TREAT
Plasmid#196931PurposeMutated mir21 binding sites (3x) were integrated in between the stop codon and TREAT cassette in IRE-TREAT reporterDepositorInsertRenilla luciferase
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
miR21-IRE-TREAT
Plasmid#196930Purposemir21 binding sites (3x) were integrated in between the stop codon and TREAT cassette in IRE-TREAT reporter.DepositorInsertRenilla luciferase
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
Let7-IRE-TREAT
Plasmid#196925PurposeLet7-miRNA binding sites (3x) were integrated in between the stop codon and TREAT cassette in IRE-TREAT reporter.DepositorInsertRenilla luciferase
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
sm-9xGCN4-Renilla-MS2
Plasmid#178009PurposeExpresses Spaghetti Monster (sm) 9xGCN4-Renilla mRNA reporter in mammalian cellsDepositorInsertsm-9xGCN4
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
IRE-TPI-wildtype-TREAT
Plasmid#196923PurposeThe coding sequence of Triose phosphate isomerase gene was fused downstream Renilla in the ORFDepositorInsertRenilla luciferase
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
IRE-nonOpt-TREAT
Plasmid#196922PurposeThe codon optimized Renilla coding sequence in the IRE-TREAT reporter was replaced by non-optimal Renilla containing 93 synonymous codon substitutions.DepositorInsertnon-optimal Renilla luciferase
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
Let7R-IRE-TREAT
Plasmid#196934PurposeReverse complement of let7-miRNA binding sites (3x) were integrated in between the stop codon and TREAT cassette in IRE-TREAT.DepositorInsertRenilla luciferase
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR U6 L1-R5
Plasmid#62111PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter for shRNA expression. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseRNAi; Mule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR H1 L5-L4
Plasmid#62102PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a H1 promoter for shRNA expression. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseRNAi; Mule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR U6 L1-L4
Plasmid#62112PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter for shRNA expression. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseRNAi; Mule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR H1 L5-L2
Plasmid#62103PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a H1 promoter for shRNA expression. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseRNAi; Mule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only