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pscAAV.T7-SP6-BC(p11-14)-CAG-EGFP-SV40pA-T7

Plasmid

#231350

Purpose

Self complementary AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses EGFP from CAG promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

T7-SP6-BC(p11-14)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.T7-SP6-BC(p11-14)-mDLX-minBG-CI-mRuby2-W3SL-T7

Plasmid

#231352

Purpose

Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from minBG promoter with mDLX enhancer.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

T7-SP6-BC(p11-14)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.T7-SP6-BC(p11-14)-minBG-CI-mRuby2-W3SL-T7

Plasmid

#231353

Purpose

Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from minBG promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

T7-SP6-BC(p11-14)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.T7-SP6-BC(p11-14)-SCP1-tdTomato-W3SL-T7

Plasmid

#231356

Purpose

Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses tdTomato from SCP1 promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

T7-SP6-BC(p11-14)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.T7-SP6-BC(p11-14)-CMVp-CI-mRuby2-W3SL-T7

Plasmid

#231357

Purpose

Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from CMV promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

T7-SP6-BC(p11-14)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.T7-SP6-BC(p11-14)-Ef1s-CI-mRuby2-W3SL-T7

Plasmid

#231358

Purpose

Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from Ef1s promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

T7-SP6-BC(p11-14)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.T7-SP6-BC(p11-14)-SCP1-CI-mRuby2-W3SL-T7

Plasmid

#231359

Purpose

Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from SCP1 promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

T7-SP6-BC(p11-14)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.T7-SP6-BC(p11-14)-CAG-EGFP-W3SL-T7

Plasmid

#231348

Purpose

Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses EGFP from CAG promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

T7-SP6-BC(p11-14)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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BII-ChBtW-mADAR2-short

Plasmid

#175588

Purpose

mammalian expression of mouse short Adar2, blasticidine selection

Depositor

Emanuela Felley-Bosco

Article

Hariharan et al Mol Oncol. 2022 Dec;16(22):3949-3974. do

Insert

Short Mouse Adar2

Tags

Flag

Expression

Mammalian

Promoter

cmv

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pmirGlo-RBM8A-3'UTR AluJo-Y 240-2996

Plasmid

#171131

Purpose

reporter

Depositor

Emanuela Felley-Bosco

Article

Abukar et al Cells. 2021 Dec 15;10(12). pii: cells101

Insert

RBM8A 3'UTR (RBM8A Human)

Expression

Mammalian

Available Since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pmirGlo-RBM8A-3'UTR AluSz 3501-4065

Plasmid

#171132

Purpose

reporter

Depositor

Emanuela Felley-Bosco

Article

Abukar et al Cells. 2021 Dec 15;10(12). pii: cells101

Insert

RBM8A 3'UTR (RBM8A Human)

Expression

Mammalian

Available Since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pmirGlo-RBM8A-3'UTR AluY WT 2598-2996

Plasmid

#171134

Purpose

reporter

Depositor

Emanuela Felley-Bosco

Article

Abukar et al Cells. 2021 Dec 15;10(12). pii: cells101

Insert

RBM8A 3'UTR (RBM8A Human)

Expression

Mammalian

Available Since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pmirGlo-RBM8A-3'UTR AluY-Sz WT 2598-4066

Plasmid

#171136

Purpose

reporter

Depositor

Emanuela Felley-Bosco

Article

Abukar et al Cells. 2021 Dec 15;10(12). pii: cells101

Insert

RBM8A 3'UTR (RBM8A Human)

Expression

Mammalian

Available Since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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ppyCAG_RNaseH1_WKKD

Plasmid

#111905

Purpose

Express WKKD mutated RNASEH1 in mammalian cell. The combination of three specific mutations (W43A, K59A, K60A) in the binding domain prevents the enzyme from binding to RNA/DNA hybrids

Depositor

Xiang-Dong Fu

Article

Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.

Insert

RNASEH1 (RNASEH1 Human)

Tags

V5

Expression

Mammalian

Mutation

Changed D 210 to N, W 43 to A, K 59 to A, K 60 to…

Available Since

July 4, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PBZ-mRuby2

Plasmid

#110650

Purpose

expresses a PAR-binding (PBZ) domain fused to mRuby2

Depositor

Chris Lord

Article

Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

Insert

PBZ domain from APLF, amino acids 371-451 (APLF Human)

Tags

mRuby2

Expression

Mammalian

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-sumo-NSP12 (SARS-CoV-2)

Plasmid

#159107

Purpose

The SARS-CoV-2 NSP12 coding sequence was cloned into a modified pRSFDuet-1 vector (Novagen) bearing an N-terminal His6-SUMO-tag which is cleavable by the ubiquitin-like protease (ULP1).

Depositor

Thomas Tuschl

Article

Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

Insert

NSP12

Tags

His-SUMO tag

Expression

Bacterial

Mutation

None

Promoter

T7

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a 6xHis PreScission SARS-CoV-2 nsp13

Plasmid

#159390

Purpose

Expresses the SARS-CoV-2 nsp13 protein with an N-terminal His6 tag followed by an HRV 3C/PreScission cleavage site.

Depositor

Tarun Kapoor

Article

Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

Insert

N-terminal His tag PreScission SARS-CoV-2 nsp13, optimized for insect cell expression

Tags

His6 tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pBiFC-PBZ-VN

Plasmid

#110646

Purpose

expresses a PAR-binding (PBZ) domain fused to venus N-terminus

Depositor

Chris Lord

Article

Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

Insert

PBZ domain from APLF, amino acids 371-451 (APLF Human)

Tags

DDK and VN

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pET15-ZmPYK

Plasmid

#220230

Purpose

Inducible expression of Zymomonas mobilis pyruvate kinase

Depositor

Aron Fenton, Liskin Swint-Kruse

Article

Swint-Kruse et al Database (Oxford). 2023 May 3;2023:baad0

Insert

Zymomonas mobilis pyruvate kinase (pyk, ZMO0152 Zymomonas mobilis)

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pCI-Puro_mouse_Adar2_Tv1

Plasmid

#158111

Purpose

expresses mADAR2 shortest transcript, puromycin selection

Depositor

Emanuela Felley-Bosco

Article

Abukar et al Cells. 2021 Dec 15;10(12). pii: cells101

Insert

adar2 (Adarb1 Mouse)

Expression

Mammalian

Available Since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge