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We narrowed to 730 results for: bacillus

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Showing: 381 - 400 of 730 results

  3. pET28a-HIS-Xyl-Xmod-DocIII

    Plasmid
    #60865
    Purpose
    Characteristic fingerprint molecule fused to dockerin type III domain to perform single molecule force spectroscopy experiments; covalently immobilized via introduced Cystein in Xylanase T120C
    Depositor
    Hermann Gaub
    Publication
    Schoeler et al Nat Commun. 2014 Dec 8;5:5635. doi: 10.1
    Inserts
    Xyl
    Xmod-DocIII
    Tags
    6xHIS
    Expression
    Bacterial
    Mutation
    Threonine 120 to Cysteine in Xylanase domain
    Promoter
    T7
    Available since
    Nov. 26, 2014
    Availability
    Academic Institutions and Nonprofits only
    60865-plasmid-map-sequence-id-98100

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  4. pADsacA

    Plasmid
    #158649
    Purpose
    Constitutive transcription of sacA crRNA and GFP
    Depositor
    Wenjing Cui
    Insert
    sacA crRNA
    Use
    CRISPR and Synthetic Biology; Shuttle vector baci…
    Expression
    Bacterial
    Promoter
    Pveg
    Available since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    158649-plasmid-map-sequence-id-310569

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  6. pU6-SacB-Cas9-T2A-mCherry_(BbsI)

    Plasmid
    #117070
    Purpose
    Encodes Cas9, T2A-mCherry, and AmpR which creates a CRISPR system application with reliable positive selection
    Depositor
    Kim Failor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9
    SacB
    Use
    CRISPR
    Tags
    3x FLAG, NLS, and T2A-mCherry
    Expression
    Mammalian
    Promoter
    CBh and U6
    Available since
    Dec. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    117070-plasmid-map-sequence-id-231696

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  10. YE2-BE4max

    Plasmid
    #138156
    Purpose
    C-to-T base editor
    Depositor
    David Liu
    Publication
    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    rAPOBEC1(YE2)-nSpCas9-UGI-UGI
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Within rAPOBEC1 W90Y + R132E; within Cas9 D10A
    Promoter
    CMV
    Available since
    Feb. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    138156-plasmid-map-sequence-id-272343

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  15. p3R112C/T180CBM3

    Plasmid
    #85090
    Purpose
    Expresses PCNA3 variant (R112C/T180C) fused to P450 BM3 variant (A74G/C773S/C810S) in E. coli
    Depositor
    Teruyuki Nagamune
    Publication
    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi
    Insert
    The R112C/T180C variant of PCNA3 fused to P450 BM3
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    R112C/T180C in PCNA3, A74G/C773S/C810S in P450BM3
    Promoter
    T7 promoter
    Available since
    Dec. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    85090-plasmid-map-sequence-id-164484

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  16. YE1-BE4max-CP1028

    Plasmid
    #138160
    Purpose
    C-to-T base editor
    Depositor
    David Liu
    Publication
    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    rAPOBEC1(YE1)-nSpCas9 (CP1028) -UGI-UGI
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Within rAPOBEC1 W90Y + R126E; within Cas9 (CP1028…
    Promoter
    CMV
    Available since
    Feb. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    138160-plasmid-map-sequence-id-272345

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  17. nCas9-UGI-NLS-P2A-EGFP (pJUL1001)

    Plasmid
    #123611
    Purpose
    CAG promoter expression plasmid for hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (BE3 control without rAPOBEC1).
    Depositor
    Keith Joung
    Publication
    Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nCas9-UGI-NLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A in SpCas9
    Promoter
    CAG
    Available since
    April 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    123611-plasmid-map-sequence-id-239339

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  18. pBsacA

    Plasmid
    #158650
    Purpose
    Constitutive transcription of sacA crRNA
    Depositor
    Wenjing Cui
    Insert
    sacA crRNA
    Use
    CRISPR and Synthetic Biology; Shuttle vector baci…
    Expression
    Bacterial
    Promoter
    Pveg
    Available since
    Oct. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    158650-plasmid-map-sequence-id-314145

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  19. AALN-BE4max

    Plasmid
    #138161
    Purpose
    C-to-T base editor
    Depositor
    David Liu
    Publication
    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    rAPOBEC1(AALN)-nSpCas9-UGI-UGI
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Within rAPOBEC1 R33A + K34A + H122L + D124N; with…
    Promoter
    CMV
    Available since
    Feb. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    138161-plasmid-map-sequence-id-272350

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Showing: 381 - 400 of 730 results