We narrowed to 10,382 results for: plasmids 101

1. GEARBOCS-SPARCL1-GeneTRAP

   Plasmid

   #218184

   Purpose

   To KO and genetrap mouse Hevin simultaneously

   Depositor

   Cagla Eroglu

   Article

   Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

   Insert

   sgRNA (Sparcl1 Mouse)
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCJH009_proD_Cas12c-sgRNA-GFP_CAM

   Plasmid

   #183070

   Purpose

   Plasmid expressing Cas12c sgRNA targeting GFP (1) for in vivo interference assay in bacteria.

   Depositor

   Jennifer Doudna

   Article

   Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

   Insert

   Cas12c single guide RNA targeting GFP
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pVHed02

   Plasmid

   #82352

   Purpose

   Bxb1/TP901 temporal logic gate with mKate2 and sfGFP on low-copy plasmid

   Depositor

   Richard Murray

   Article

   Hsiao et al Mol Syst Biol. 2016 May 17;12(5):869. do

   Inserts

   mKate2 RFP
   Superfolder GFP
   Temporal logic gate with TP901 and Bxb1 attachment sites
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PacrAB-rfp + PmutS-yfp

   Plasmid

   #121442

   Purpose

   plasmid reporting the expression from the acrAB and mutS promoters

   Depositor

   Mary Dunlop

   Article

   El Meouche et al Science. 2018 Nov 9;362(6415):686-690. d

   Inserts

   PmutS-yfp
   PacrAB-rfp
   

   Use

   Synthetic Biology; Pbb vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PacrAB and PmutS

   Available Since

   March 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAP635-2

   Plasmid

   #99485

   Purpose

   Myc::TEV::3XFlag::Dendra2::3XFlag::TEV::Myc

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Methods. 2017 May 15;121-122:86-93. doi:

   Insert

   Myc::TEV::3XFlag::Dendra2::3XFlag::TEV::Myc
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCJH031_proD_LbCas12a-gRNA-GFP_CAM

   Plasmid

   #183075

   Purpose

   Plasmid expressing Cas12a guide RNA targeting GFP (1) for in vivo interference assay in bacteria.

   Depositor

   Jennifer Doudna

   Article

   Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

   Insert

   LbCas12a guide RNA targeting GFP
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Pconst-rfp + PmutS-yfp

   Plasmid

   #121444

   Purpose

   plasmid reporting the expression from constitutive and mutS promoters

   Depositor

   Mary Dunlop

   Article

   El Meouche et al Science. 2018 Nov 9;362(6415):686-690. d

   Inserts

   PmutS-yfp
   Pconst-rfp
   

   Use

   Synthetic Biology; Pbb vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P-const and P-mutS

   Available Since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PacrAB-rfp + Pconst-yfp

   Plasmid

   #121445

   Purpose

   plasmid reporting the expression from acrAB and constitutive promoters

   Depositor

   Mary Dunlop

   Article

   El Meouche et al Science. 2018 Nov 9;362(6415):686-690. d

   Inserts

   Pconst-yfp
   PacrAB-rfp
   

   Use

   Synthetic Biology; Pbb vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PacrAB and Pconst

   Available Since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAP639-2F

   Plasmid

   #99486

   Purpose

   3XFlag::TEV::6XHis::mTurquoise::6XHis::TEV::3XFlag

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Methods. 2017 May 15;121-122:86-93. doi:

   Insert

   3XFlag::TEV::6XHis::mTurquoise::6XHis::TEV::3XFlag
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAP607-2

    Plasmid

    #99483

    Purpose

    Myc::TEV::3XFlag::GFP::3XFlag::TEV::Myc

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Methods. 2017 May 15;121-122:86-93. doi:

    Insert

    Myc::TEV::3XFlag::GFP::3XFlag::TEV::Myc
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDL1-Spol-Ovo-Nterm

    Plasmid

    #242942

    Purpose

    Encodes an N-terminal fragment of Spol-Ovo for use in generating dsRNA for RNAi in the planarian S. polychroa

    Depositor

    Erin Davies

    Article

    Deihl et al bioRxiv [Preprint]. 2025 Jul 22:2025.07.

    Insert

    Spol-Ovo (Nterm fragment)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDL1-Spol-Ovo-C-term

    Plasmid

    #242943

    Purpose

    Encodes a C-terminal fragment of Spol-Ovo for use in generating dsRNA for RNAi in the planarian S. polychroa

    Depositor

    Erin Davies

    Article

    Deihl et al bioRxiv [Preprint]. 2025 Jul 22:2025.07.

    Insert

    Spol-Ovo (Cterm fragment)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAP-U2-3

    Plasmid

    #236474

    Purpose

    Plasmid for integration at the native URA3 locus with 3xmCherry under control of the ScACT1 promoter and 3xGFP under control of the ApACT1 promoter.

    Depositor

    Daniel Lew

    Article

    Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

    Insert

    scACT1p-3xmCherry apACT1p-3xGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Codon optimized for A. pullulans

    Promoter

    Available Since

    April 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAP-U2-4

    Plasmid

    #236475

    Purpose

    Plasmid for integration at the native URA3 locus with 3xmCherry under control of the ScACT1 promoter and 3xGFP under control of the ApTUB1 promoter.

    Depositor

    Daniel Lew

    Article

    Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

    Insert

    scACT1p-3xmCherry apTUB1p-3xGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Codon optimized for A. pullulans

    Promoter

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAP-U2-2

    Plasmid

    #236473

    Purpose

    Plasmid for integration at the native URA3 locus with 3xmCherry under control of the SpH2B promoter and 3xGFP under control of the ScACT1 promoter.

    Depositor

    Daniel Lew

    Article

    Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

    Insert

    spH2Bp-3xmCherry scACT1p-3xGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Codon optimized for A. pullulans

    Promoter

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSliceRep_B_miR-196a.M3

    Plasmid

    #220471

    Purpose

    Dual luciferase reporter for RNAi, with target site within a tunable ORF (version B)

    Depositor

    David Bartel

    Article

    Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

    Insert

    SUMO_Eu1
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Minimal CMV

    Available Since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSliceRep_A_miR-196a.M3

    Plasmid

    #220467

    Purpose

    Dual luciferase reporter for RNAi, with target site within a tunable ORF (version A)

    Depositor

    David Bartel

    Article

    Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

    Insert

    Superfolder GFP
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Minimal CMV

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSliceRep_B_miR-196a

    Plasmid

    #220470

    Purpose

    Dual luciferase reporter for RNAi, with target site within a tunable ORF (version B)

    Depositor

    David Bartel

    Article

    Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

    Insert

    SUMO_Eu1
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Minimal CMV

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSliceRep_A_miR-7

    Plasmid

    #220464

    Purpose

    Dual luciferase reporter for RNAi, with target site within a tunable ORF (version A)

    Depositor

    David Bartel

    Article

    Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

    Insert

    Superfolder GFP
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Minimal CMV

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSliceRep_A_miR-7.M4

    Plasmid

    #220465

    Purpose

    Dual luciferase reporter for RNAi, with target site within a tunable ORF (version A)

    Depositor

    David Bartel

    Article

    Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

    Insert

    Superfolder GFP
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Minimal CMV

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge