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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,214 results for: Abo;

Showing: 3981 - 4000 of 4214 results

  1. Tet-pLKO-neo

    Plasmid
    #21916
    Purpose
    3rd generation lentiviral plasmid for inducible expression of shRNA; neomycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.
    Depositor
    Dmitri Wiederschain
    Article
    Wiederschain et al Cell Cycle. 2009 Feb 1. 8(3):498-504.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 22, 2009
    Availability
    Academic Institutions and Nonprofits only
    21916-plasmid-map-sequence-id-358974

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  4. pET28a hMDH1v3 (+TEV)

    Plasmid
    #204229
    Purpose
    Bacterial expression of human cytosolic malate dehydrogenase isoform 1 transcript variant 3 (hMDH1v3); Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    hMDH1v3 (MDH1 Human)
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Inserted a G after ATG to maintain kozak site
    Promoter
    Lac
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204229-plasmid-map-sequence-id-413334

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  9. pTet-On-TAP

    Plasmid
    #59017
    Purpose
    Construct allows tetracycline inducible expression of a protein of interest fused to a tandem affinity purification tag (TAP) in toxoplasma gondii.
    Depositor
    David Sibley
    Article
    Etheridge et al Cell Host Microbe. 2014 May 14;15(5):537
    Type
    Empty backbone
    Use
    Toxoplasma expression
    Tags
    Calmodulin Binding Peptide fused with a TEV Cleav…
    Expression
    Mutation
    Promoter
    RPS13 with Tet Operators Inserted
    Available since
    Sept. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    59017-plasmid-map-sequence-id-423220

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  12. pRVL-6

    Plasmid
    #104584
    Purpose
    Contains human IgG1 CH1/mutated CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Abolished Fc-mediated immune effector functions
    Depositor
    Daniel Christ
    Article
    Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi
    Insert
    Human IgG1 CH1/CH2/CH3 (mutated, no effector functions) (IGHG1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    L234A/L235E/G237A and K322A/P331S
    Promoter
    CMV
    Available since
    Jan. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    104584-plasmid-map-sequence-id-198608

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  13. pcDNA3-Lyso-mCherry-Rheb(C181S)

    Plasmid
    #192438
    Purpose
    Encodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant targeted to lysosome membrane.
    Depositor
    Jin Zhang
    Article
    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1
    Insert
    Lyso-mCherry-Rheb(C181S) (RHEB Human)
    Use
    Tags
    Full-length LAMP1 and mCherry
    Expression
    Mammalian
    Mutation
    Rheb Cys 181 mutated to Ser; abolishes C-terminal…
    Promoter
    CMV
    Available since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    192438-plasmid-map-sequence-id-379134

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  16. L-GARS-2

    Plasmid
    #166531
    Purpose
    scFv of a human scaffold targeting Glycyl-tRNA synthetase. Antigen coverage aa 55-739 of 739
    Depositor
    Susanne Gräslund
    Article
    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166531-plasmid-map-sequence-id-328315

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  18. pBbE1k-3

    Plasmid
    #71722
    Purpose
    tyrosine hydroxylase from mouse, dihydropterine reductase from human, pterin-4a-carbinolamine dehydratase from human
    Depositor
    Taek Soon Lee
    Article
    Satoh et al Metab Eng. 2012 Nov;14(6):603-10. doi: 1
    Inserts
    dihydropterine reductase (QDPR Human)
    pterin-4a-carbinolamine dehydratase (PCBD1 Human)
    Use
    Tags
    Expression
    Mutation
    Promoter
    pTrc
    Available since
    April 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    71722-plasmid-map-sequence-id-129984

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Showing: 3981 - 4000 of 4214 results