We narrowed to 21,256 results for: KIN

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pBabe puro Myr HA PIK3CB

Plasmid

#12527

Depositor

Jean Zhao

Article

Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

Insert

PIK3CB (PIK3CB Human)

Use

Retroviral

Tags

HA and Myr

Expression

Mammalian

Mutation

Myristoylation. Constitutively active allele.

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCDH-CRY2-mCh-superPLDx30

Plasmid

#188988

Purpose

A lentiviral plasmid encoding CRY2-mCh-superPLDx30 (an engineered PLD mutant with 30 times higher activity than the wild-type)

Depositor

Jeremy Baskin

Article

Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

Insert

PLD

Tags

CRY2 and mCherry

Expression

Mammalian

Mutation

K109R, P245A, G328S, G381V, G429D

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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mEos2-CD9-10

Plasmid

#57356

Purpose

Localization: Tetraspanin/Membrane, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

CD9 (CD9 Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pCR3.1 GSK3beta S9A HA

Plasmid

#15994

Depositor

Morris Birnbaum

Article

Summers et al J Biol Chem. 1999 Jun 18. 274(25):17934-

Insert

GSK3beta (GSK3B Human)

Tags

HA

Expression

Mammalian

Mutation

S9A: constitutively active

Available Since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only
pJSK418

Plasmid

#205131

Purpose

Expression of GFP-2xFYVE in Dictyostelium

Depositor

Jason King

Article

Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

Insert

2xFYVE (from human Hrs) (HGS Synthetic)

Use

Dictyostelium expression

Tags

EGFP

Available Since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV_tKiMBI-T2A-caMEK

Plasmid

#199578

Purpose

Expresses tKiMBI and caMEK in an AAV vector

Depositor

Michael Lin

Article

Wu et al ACS Cent Sci. 2023 Mar 20;9(4):719-732.

Inserts

ERK tdTomato-Kinase-Modulated Bioluminescent Indicator
constitutively active MEK

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-CIBN-CAAX

Plasmid

#188989

Purpose

A lentiviral plasmid encoding plasma membrane-tagged CIBN

Depositor

Jeremy Baskin

Article

Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

Insert

CIBN (CIB1 Mustard Weed)

Tags

CAAX

Expression

Mammalian

Promoter

CMV

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pET28_human-pro-IL-18_DeltaN24

Plasmid

#214327

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Tags

His-TEV

Expression

Bacterial

Mutation

Deletion of first 24 amino acids

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac_human-pro-IL-18

Plasmid

#214337

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_Pro-IL-36γ

Plasmid

#214345

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL36G (IL36G Human)

Tags

His-TEV and Myc

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pFUGW-ZipACR-EYFP

Plasmid

#88844

Purpose

Fast anion channelrhodopsin (current half-decay time 2-4 ms depending on voltage) for time-resolved inhibition of neuronal spiking

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

ZipACR-NotI-EYFP

Use

Lentiviral

Tags

EYFP

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pcdna3 Flag Jip2

Plasmid

#59027

Purpose

act as molecular scaffolds that mediate the activation of the JNK signaling pathway in vivo.

Depositor

Roger Davis

Article

Yasuda et al Mol Cell Biol. 1999 Oct;19(10):7245-54.

Insert

mitogen-activated protein kinase 8 interacting protein 2 (MAPK8IP2 )

Tags

Flag

Expression

Mammalian

Available Since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only
isoform PKC lambda (JCE590)

Plasmid

#89775

Purpose

yeast expression of mouse PKC lambda with yeast PKC1 promoter and ADH1 terminator

Depositor

Juan Carlos Igual

Article

Soriano-Carot et al Nucleic Acids Res. 2014 Jun;42(11):7084-

Insert

PKC lambda (Prkci Mouse)

Expression

Mammalian

Promoter

yeast PKC1 promoter (-600 to -1)

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNAIIIB cdc42 WT

Plasmid

#61635

Purpose

mammalian expression of cdc42 wt

Depositor

JS Gutkind

Article

Coso et al Cell. 1995 Jun 30;81(7):1137-46.

Insert

cdc42 (CDC42 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pPBbsr2-5102HRasCT(Picchu)

Plasmid

#209917

Purpose

A FRET biosensor for tyrosine kinase activity

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

5102HRasCT

Tags

YPet and nTurquoise-GL

Expression

Mammalian

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 TGFB1

Plasmid

#67080

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TGFB1 (TGFB1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 CALR

Plasmid

#67086

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

CALR (CALR Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pLHCX-Flag-mPKM2(K433E)

Plasmid

#42514

Depositor

Dimitrios Anastasiou

Article

Anastasiou et al Science. 2011 Dec 2;334(6060):1278-83. d

Insert

Pyruvate Kinase M2 Lys433->Glu

Use

Retroviral

Tags

Flag

Mutation

Changed lysine 433 to glutamate

Available Since

March 19, 2013

Availability

Academic Institutions and Nonprofits only
ROZA-XL

Plasmid

#64194

Purpose

Fluorescent reporter for ZAP-70 tyrosine kinase activity

Depositor

Annemarie Lellouch

Article

Cadra et al Biochem Biophys Res Commun. 2015 Feb 28.

Insert

ROZA-XL

Expression

Mammalian

Mutation

ROZA-XL

Promoter

CMV

Available Since

April 23, 2015

Availability

Academic Institutions and Nonprofits only
pENTR-Arl13b2

Plasmid

#40871

Depositor

Tamara Caspary

Article

Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

Insert

Arl13b (Arl13b Mouse)

Use

Gateway entry

Mutation

no stop codon

Available Since

Nov. 27, 2012

Availability

Academic Institutions and Nonprofits only