We narrowed to 8,159 results for: Dos;

1. pCMV-T7-AcrIIA16_Efa-NLS(SV40) (KAC459)

   Plasmid

   #134337

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AcrIIA16_Efa with C-terminal NLS (SV40)

   Depositor

   Joseph Bondy-Denomy, Benjamin Kleinstiver

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized AcrIIA16_Efa
   

   Use

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCPH2A.X_S139A-GS3

   Plasmid

   #204749

   Purpose

   Expression of Cas9 and human H2AX with the S139A mutation

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

   Insert

   H2AX S139A (H2AX Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   H2AX with the S139A mutation

   Promoter

   Available since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKESaPar

   Plasmid

   #211829

   Purpose

   Low copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.

   Depositor

   Anita Emmerstorfer-Augustin

   Article

   Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

   Insert

   eGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   Feb. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Human a4 nicotinic subunit crystallization construct

   Plasmid

   #85841

   Purpose

   pEZT-BM vector with alpha4 gene after CMV promoter, published crystallization construct

   Depositor

   Ryan Hibbs

   Article

   Morales-Perez et al Nature. 2016 Oct 3;538(7625):411-415. do

   Insert

   Human a4 nAChR subunit crystallization construct (CHRNA4 Human)
   

   Use

   Baculovirus (bacmam) production and mammalian exp…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGWB405-TIC21-sGFP

   Plasmid

   #213865

   Purpose

   Binary vector for the expression of sGFP flanked with an N-terminal chloroplas inner envelope membrane transit peptide in plants .

   Depositor

   Yutaka Kodama

   Article

   Ichikawa et al Plant Direct. 2022 Nov 15;6(11):e462. do

   Insert

   chloroplast inner envelope membrane transit peptide (TIC21 Mustard Weed)
   

   Use

   Tags

   Expression

   Bacterial and Plant

   Mutation

   Promoter

   Available since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. CMV PGK1-3xHA

   Plasmid

   #220913

   Purpose

   Expresses PGK1-3xHA

   Depositor

   Timothy Ryan

   Article

   Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

   Insert

   PGK1-3xHA (PGK1 Human)
   

   Use

   Affinity Reagent/ Antibody

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. TPST1-EGFP

   Plasmid

   #66617

   Purpose

   Encodes GFP fusion of TPST1 trans Golgi marker; (Tyrosyl protein sulfotransferase 2)

   Depositor

   David Stephens

   Article

   Spooner et al Biochem J. 2008 Sep 15;414(3):471-84. do

   Insert

   TPST1 (TPST1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pHH0103_TXN_Thioredoxin

   Plasmid

   #109778

   Purpose

   Protein expression and purification of TXN_Thioredoxin

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   TXN_Thioredoxin (TXN Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-T7-AcrIIA16_Lmo-NLS(SV40) (KAC508)

   Plasmid

   #134332

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AcrIIA16_Lmo with C-terminal NLS (SV40)

   Depositor

   Joseph Bondy-Denomy, Benjamin Kleinstiver

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized AcrIIA16_Lmo
   

   Use

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-T7-AcrIIC1-NLS(SV40) (KAC208)

    Plasmid

    #134340

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIC1 with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized AcrIIC1
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCS2-mCherry-Cenexin

    Plasmid

    #186435

    Purpose

    Expresses mCherry Cenexin in mammalian cells and zebrafish embryos

    Depositor

    Heidi Hehnly

    Article

    Aljiboury et al Mol Biol Cell. 2022 Aug 1;33(9):br14. do

    Insert

    Cenexin (ODF2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. EGFP-BAF_L58R

    Plasmid

    #101776

    Purpose

    Expresses EGFP tagged mutant BAF (L58R) in human cells

    Depositor

    Daniel Gerlich

    Article

    Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

    Insert

    BAF (BANF1) (BANF1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    L58R mutation

    Promoter

    EF1a

    Available since

    Jan. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. mCherry-iLIDx6

    Plasmid

    #103779

    Purpose

    expresses iPOLYMER component in mammalian cells

    Depositor

    Takanari Inoue

    Article

    Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

    Insert

    mCherry-iLIDx6
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEGFP-C1 RPEL1-EGFP-3XNLS

    Plasmid

    #58469

    Purpose

    Expresses a nuclear-targeted monomeric actin reporter, consisting of the RPEL1 domain from MAL/MRTF, on a CMV promoter

    Depositor

    Dyche Mullins

    Article

    Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do

    Inserts

    MRTF-A RPEL1 (Mrtfa Synthetic, Mouse)
    3X NLS with 3X stop codons
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. mVenus-IQ6 R6A

    Plasmid

    #198202

    Purpose

    FRET acceptor: Mutated IQ6 domain of Moysin Va tagged with N-terminal mVenus (lower binding affinity)

    Depositor

    Christian Wahl-Schott

    Article

    Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

    Insert

    MYO5A IQ domain (Myo5a Mouse)
    

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    R6A

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. Human b2 nicotinic subunit crystallization construct

    Plasmid

    #85842

    Purpose

    pEZT-BM vector with beta2 gene after CMV promoter, published crystallization construct

    Depositor

    Ryan Hibbs

    Article

    Morales-Perez et al Nature. 2016 Oct 3;538(7625):411-415. do

    Insert

    Human b2 nAChR subunit crystallization construct (CHRNB2 Human)
    

    Use

    Baculovirus (bacmam) production and mammalian exp…

    Tags

    C-terminal StrepII tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX335-EN475

    Plasmid

    #86231

    Purpose

    For transient expression of spCas9-nickase and one sgRNA targeting the mouse CTCF locus. Use together with pX335-EN477.

    Depositor

    Benoit Bruneau

    Article

    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

    Insert

    spCas9-nickase and sgRNA against mouse CTCF STOP Codon
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. YFP-SspBx6

    Plasmid

    #103778

    Purpose

    expression of iPOLYMER component in mammalian cells

    Depositor

    Takanari Inoue

    Article

    Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

    Insert

    YFP-SspBx6
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. 2xHP1bCD_W42A-IFP2.0C

    Plasmid

    #215751

    Purpose

    Expresses binding-deficient W42A mutant of 2xHP1beta chromo domain fused to C-terminal part of IFP2.0 as negative control for H3K9me2/3 detection in BiAD sensors

    Depositor

    Albert Jeltsch

    Article

    Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

    Insert

    2xHP1bCD_W42A-IFP2.0C
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pX335-EN477

    Plasmid

    #86232

    Purpose

    For transient expression of spCas9-nickase and one sgRNA targeting the mouse CTCF locus. Use together with pX335-EN475.

    Depositor

    Benoit Bruneau

    Article

    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

    Insert

    spCas9-nickase and sgRNA against mouse CTCF STOP Codon
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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