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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,214 results for: LEA

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Showing: 4001 - 4020 of 12214 results

  3. pCL1271

    Plasmid
    #217963
    Purpose
    Mammalian expression of Importin 5 (Imp5, IPO5) with an N-terminal eGFP fusion. Splice isoform 1.
    Depositor
    Andrew Bowman
    Publication
    Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Imp5 (IPO5 Human)
    Tags
    eGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    217963-plasmid-map-sequence-id-430217

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  6. pCAG-AsCas12a-Plus-mCherry

    Plasmid
    #191231
    Purpose
    To express the AsCas12a nuclease variant "AsCas12a-Plus" that enhanced the editing activity and specificity
    Depositor
    Zhili Rong
    Publication
    Huang et al BMC Biol. 2022 Apr 25;20(1):91. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AsCas12a-Plus-P2A-mCherry
    Use
    CRISPR
    Mutation
    E174R, R951K, R955A
    Available since
    May 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    191231-plasmid-map-sequence-id-392988

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  15. pOCC267

    Plasmid
    #118887
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal HALO tag
    Depositor
    Marc Bickle, PEPC MPI CBG
    Publication
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-MBP-3C-HALO-NotI-ccdB-AscI-stop-HindIII cassette
    Tags
    HIS6-MBP, cleavable with 3C protease; HALO tag
    Expression
    Insect
    Promoter
    polH
    Available since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118887-plasmid-map-sequence-id-232249

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  16. PH-dL5**

    Plasmid
    #96946
    Purpose
    PLC pleckstrin homology domain linked to FAP for CALI validation
    Depositor
    Marcel Bruchez
    Publication
    He et al Nat Methods. 2016 Mar;13(3):263-268. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Pleckstrin homology domain
    Tags
    dL5 and mCerulean
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    96946-plasmid-map-sequence-id-210240

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  18. CFL1 (CFL1A-c002)

    Plasmid
    #98237
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CFL1A (CFL1 Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    pfam00241:Cofilin_ADF 27-148
    Promoter
    T7
    Available since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98237-plasmid-map-sequence-id-193416

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Showing: 4001 - 4020 of 12214 results