We narrowed to 12,112 results for: LEA
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Plasmid#178734PurposeN-myristoylation signaling peptide of Src and the C-terminal fragment of G-protein coupled receptor kinase 2 (ct-GRK2)DepositorInsertGRK2 (GRK2 Bovine)
UseLentiviralTagsSrc 1-15Mutationonly amino acids 548-671PromoterUbiquitinAvailable sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SynI-FlpOn-CreOff-TdTomato
Plasmid#176275PurposeViral vector for expression of TdTomato in cells expressing Flp AND NOT Cre driven by the human Synapsin promoter.DepositorInserttdTomato
UseAAV and Cre/LoxExpressionMammalianPromoterhuman Synapsin IAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV_UdgX-APOBEC1-UdgX-nCas9
Plasmid#163555PurposeMammalian CG-to-GC base editingDepositorInsertUdgX-APOBEC1-UdgX-nCas9
UseCRISPRExpressionMammalianMutationnCas9 (D10A)Available sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pASPIre2
Plasmid#154843PurposeDerivative of pASPIre1 carrying a bxb1-sfGFP translational fusion in place of bxb1DepositorInsertBxb1-sfGFP fusion controlled by rhamnose-inducible promoter; attB/attP-flanked discriminator containing mCherry; rhaRS operon
ExpressionBacterialAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLRC1-NEK10p:NEK10-3XFLAG
Plasmid#137030PurposeLentiviral vector for expressing NEK10 under the control of the endogenous NEK10 promoterDepositorInsertNIMA-like kinase 10 (NEK10 Human)
UseLentiviralTags3XFLAGPromoterhuman NEK10 endogenous promoterAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_CtCHAD
Plasmid#127785PurposeExpresses CtCHAD in E. coli cellsDepositorInsertCtCHAD (CT0884 Chlorobium tepidum)
Tags6xHis tag + TEV cleavage siteExpressionBacterialMutationResidues 208-522 (CHAD domain)Available sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-V5-Smpd4
Plasmid#120237PurposeExpresses V5-tagged Smpd4 in lentiviral vectorDepositorAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC267
Plasmid#118887Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal HALO tagDepositorInsertNcoI-HIS6-MBP-3C-HALO-NotI-ccdB-AscI-stop-HindIII cassette
TagsHIS6-MBP, cleavable with 3C protease; HALO tagExpressionInsectPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
HS-aPKC-CAAX-GFP
Plasmid#105946Purposeheat shock inducible transgenesisDepositorAvailable sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
CFL1 (CFL1A-c002)
Plasmid#98237PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertCFL1A (CFL1 Human)
TagsHis-6-TEVExpressionBacterialMutationpfam00241:Cofilin_ADF 27-148PromoterT7Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQCXIX_sh_HOIP/RNF31_#6
Plasmid#75252Purposeretroviral shRNA vector against human HOIP/RNF31, expresses GFP for transduction controlDepositorAvailable sinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNB330
Plasmid#60147PurposeDisintegrator plasmid encoding MET17 promoter driven NanoLuc (Promega) with nuclear localization signal (NLS)DepositorInsertNLuc
TagsNuclear localization signal (NLS)ExpressionYeastPromoterMET17prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRVdG-N-P-M-nucBFP-EGFP-L
Plasmid#52485PurposeExpresses nuclear mTagBFP and EGFPDepositorInsertsnuclear mTagBFP
EGFP
UseRabies virusExpressionMammalianPromoterCMVAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-29b(+)_NSP4ss_SerM_NucA-myc
Plasmid#218191PurposeBacterial expression of a periplasmically expressed myc-tagged Serratia Marcescens NucA (Benzonase)DepositorInsertnucA
Tags2xmyc and NSP4 signal secretion peptideExpressionBacterialMutationcodon optimized for E. coli, residues 22-266PromoterT7Available sinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX601-AAV-CMV-NLS-ISDge10-NLS-3xHA-bGHpA;U6-BsaI-reRNA
Plasmid#205318PurposeAll-in-one AAV vector expressing ISDge10 TnpB and its reRNADepositorInserthumanized ISDge10 TnpB
UseAAVTags3 x HAExpressionMammalianAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-mNeongreen-DARPinOFF7
Plasmid#182415PurposeBacterial expression of Vesicle Nucleating peptide-mNeongreen-DARPinOFF7 fusionDepositorInsertVNp-mNeongreen-DARPinOFF7
TagsVesicle Nucleating peptide (VNp) & mNeongreenExpressionBacterialPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti-GFAP-CRY2PHR-P2A-CIBN-mCherry-VAMP2
Plasmid#178575PurposeLentiviral vector for GFAP::Opto-vTrapDepositorInsertCRY2PHR-P2A-CIBN-mCherry-VAMP2
UseLentiviralPromoterGFAPAvailable sinceApril 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-LacI-HIPK2
Plasmid#179269PurposeExpresses GFP-LacI-HIPK2 fusion proteinDepositorInserthomeodomain interacting protein kinase 2 (HIPK2 Human)
TagsGFP-LacIExpressionMammalianPromoterCMVAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SynI-FlpOn-CreOff-MCS-P2A-mCherry
Plasmid#176277PurposeViral vector for co-expression of a CDS of interest and mCherry in cells expressing Flp AND NOT Cre driven by a Synapsin promoter. Contains an in-frame P2A sequence and an MCS for cloning of the CDS.DepositorInsertMCS-P2A-mCherry
UseAAV and Cre/LoxExpressionMammalianPromoterhuman Synapsin IAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJCC_075 AsCas12a D908A
Plasmid#171668PurposeFor bacterial expression of AsCas12a D908A (RuvC-inactivating mutation) with an N-terminal His-MBP tagDepositorInsertAsCas12a D908A
Tags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationchanged aspartate 908 to alanineAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by