We narrowed to 5,560 results for: chia

1. pEIAV-SIN6.1 CZW

   Plasmid

   #44172

   Depositor

   John Olsen

   Article

   McKay et al Gene Ther. 2006 Apr;13(8):715-24.

   Insert

   LacZ (lacZ E. coli)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   May 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDBD-R

   Plasmid

   #50571

   Purpose

   Double Ub R-ek Beta-gal construct for ubiquitin sandwich assay, for measurement of cotranslational protein degradation, use with 50572

   Depositor

   Alexander Varshavsky

   Article

   Turner et al Science. 2000 Sep 22;289(5487):2117-20.

   Insert

   DHFR-Ubiquitin-ArgBetaGal-Ubiquitin-DHFR-Ubiquitin
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   M1R (B-gal)

   Promoter

   Gal1

   Available since

   Feb. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pMOD4 galK-GT

   Plasmid

   #19183

   Depositor

   Al Fisher

   Article

   Zhang et al BMC Dev Biol. 2008 Dec 30;8(1):119.

   Insert

   galK (galK E.coli)
   

   Use

   Recombineering

   Tags

   50 nucleotides from N-term TAP, C-term TAP, and F…

   Expression

   Mutation

   Promoter

   Available since

   Jan. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDR-GW FLIPglu-170nΔ13V

   Plasmid

   #27999

   Depositor

   Wolf Frommer

   Article

   Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

   Insert

   FLIPglu-170nΔ13V
   

   Use

   Tags

   eCFP and eYFP

   Expression

   Yeast

   Mutation

   WT binding protein (no F16A)

   Promoter

   Available since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pDR-GW FLIPglu-30μΔ13V

   Plasmid

   #27998

   Depositor

   Wolf Frommer

   Article

   Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

   Insert

   FLIPglu-30μΔ13V
   

   Use

   Tags

   eCFP and eYFP

   Expression

   Yeast

   Mutation

   D154A in mglB

   Promoter

   Available since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pECT5

   Plasmid

   #75451

   Purpose

   Plasmid that serves as the PCR template to create genomic pdt5 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate

   Depositor

   James Collins

   Article

   Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

   Insert

   pdt5
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   none

   Available since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. tet operator

   Plasmid

   #8901

   Depositor

   Lynda Chin

   Article

   Chin et al Nature. 1999 Jul 29. 400(6743):468-72.

   Insert

   tet operator
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pDR-GW FLIPglu-2µΔ13V

   Plasmid

   #28000

   Depositor

   Wolf Frommer

   Article

   Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

   Insert

   FLIPglu-2µΔ13V
   

   Use

   Tags

   eCFP and eYFP

   Expression

   Yeast

   Mutation

   H152A

   Promoter

   Available since

   June 10, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pICXC3H

   Plasmid

   #49022

   Purpose

   FX cloning Pichia pastoris expression vector with AOX1 promoter and C-terminal 3C protease cleavage site and 10x His tag

   Depositor

   Raimund Dutzler, Eric Geertsma

   Article

   Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

   Type

   Empty backbone

   Use

   Tags

   3C protease site (PreScission site) and decaHis (…

   Expression

   Yeast

   Mutation

   Promoter

   AOX1

   Available since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAW209

    Plasmid

    #113188

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p3XFlag-YedK

    Plasmid

    #84567

    Purpose

    Expresses YedK in mammalian cells

    Depositor

    Douglas Feldman

    Article

    Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

    Insert

    YedK
    

    Use

    Tags

    3xFlag and C-Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJT103

    Plasmid

    #25087

    Depositor

    Christopher Voigt

    Article

    Tabor et al Cell. 2009 Jun 26. 137(7):1272-81.

    Insert

    PompC-lacZ
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMal-EcPhr

    Plasmid

    #136244

    Purpose

    Protein expression of E. coli Phr

    Depositor

    Aziz Sancar

    Article

    Selby et al Proc Natl Acad Sci U S A. 2006 Nov 21. 1

    Insert

    EcPhr
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    V115E- Please see depositor comments below

    Promoter

    Available since

    Jan. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFF746

    Plasmid

    #61451

    Purpose

    aTc-inducible SCRIBE_galK(ON)

    Depositor

    Timothy Lu

    Article

    Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

    Insert

    SCRIBE_galK(ON)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    pTetO

    Available since

    March 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBad-HPII TAG392

    Plasmid

    #105848

    Purpose

    expresses E. coli HPII catalase with TAG stop codon at site 392 and N-terminal 6x his tag

    Depositor

    Ryan Mehl

    Article

    Mehl et al 2020

    Insert

    hydroperoxidase II catalase
    

    Use

    Tags

    6x his tag

    Expression

    Bacterial

    Mutation

    TAG stop codon at site 392

    Promoter

    pBad/arabinose

    Available since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDL14

    Plasmid

    #17837

    Depositor

    Jay Keasling

    Article

    Lubertozzi et al Appl Microbiol Biotechnol. 2006 Oct . 72

    Insert

    gpdA promoter, lacZ, trpC
    

    Use

    Synthetic Biology; A. nidulans

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 28, 2008

    Availability

    Academic Institutions and Nonprofits only

17. pEG3a

    Plasmid

    #31398

    Depositor

    Kenn Gerdes

    Article

    Ebersbach et al Mol Microbiol. 2008 May . 68(3):720-35.

    Insert

    zapB (zapB E. coli)
    

    Use

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pEF/myc/ER FLIPglu-3.2mDelta13V

    Plasmid

    #18019

    Depositor

    Wolf Frommer

    Article

    Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

    Insert

    FLIPglu F16A S112A Delta13
    

    Use

    Tags

    ECFP and Venus

    Expression

    Mammalian

    Mutation

    F16A, S112A in binding protein

    Promoter

    Available since

    Feb. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pECT1

    Plasmid

    #75446

    Purpose

    Plasmid that serves as the PCR template to create genomic pdt1 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate

    Depositor

    James Collins

    Article

    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

    Insert

    pdt1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    none

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBad-HPII TAG415

    Plasmid

    #105847

    Purpose

    expresses E. coli HPII catalase with TAG stop codon at site 415 and N-terminal 6x his tag

    Depositor

    Ryan Mehl

    Article

    Mehl et al 2020

    Insert

    hydroperoxidase II catalase
    

    Use

    Tags

    6x his tag

    Expression

    Bacterial

    Mutation

    TAG stop codon at site 415

    Promoter

    pBad/arabinose

    Available since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge