We narrowed to 12,680 results for: sequence

1. pFX-Lifeact-Sirius

   Plasmid

   #174475

   Purpose

   Express Sirius with actin-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   Sirius with actin-targeting sequence
   

   Use

   Tags

   Lifeact (N-terminal 17 amino acids from S. Cerevi…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPLN-hFluc-WT-eGFP-KDEL

   Plasmid

   #177727

   Purpose

   Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention and fused to eGFP at the C-terminus

   Depositor

   Erik Böttger

   Article

   Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

   Insert

   hFluc
   

   Use

   Tags

   KDEL, Prolactin Signal Sequence, and eGFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBS-V9

   Plasmid

   #173797

   Purpose

   pBS plasmid containing aadA gene with 16S rRNA promoter and psbA 3’ region, Silene noctiflora ClpP1 gene with the tobacco regulatory elements and flanking regions for tobacco plastome transformation

   Depositor

   Daniel Sloan

   Article

   Abdel-Ghany et al Plant Mol Biol. 2022 Feb;108(3):277-287.

   Inserts

   aadA
   ClpP1
   

   Use

   Tags

   Expression

   Bacterial and Plant

   Mutation

   Promoter

   Available since

   Sept. 15, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pcDNA3.1-Flag-stm2585-gp130

   Plasmid

   #174393

   Purpose

   Chimera of codon-optimized stm2585 (sarA/steE) and human gp130 sequence.

   Depositor

   Dennis Ko

   Article

   Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

   Insert

   sarA-IL6ST chimera
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   140-177 of sarA replaced with homologous IL6ST se…

   Promoter

   Available since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Ash1-5p

   Plasmid

   #169712

   Purpose

   Bacterial Expression of truncated yeast Ash1 including encoding amino acids 420-480 with phosphorylation sites 429 and 470 removed

   Depositor

   Tanja Mittag

   Article

   Martin et al J Am Chem Soc. 2016 Nov 30;138(47):15323

   Insert

   Ash1 (ASH1 Budding Yeast)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Truncation including encoding amino acids 420-480…

   Promoter

   T7

   Available since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMBP-HRV3C-AcrIF9

   Plasmid

   #141442

   Purpose

   Expression of AcrIF9 in E. coli, tagged with an HRV3C-cleavage fusion to Maltose Binding Protein.

   Depositor

   Blake Wiedenheft

   Article

   Hirschi et al Nat Commun. 2020 Jun 1;11(1):2730. doi:

   Insert

   AcrIF9
   

   Use

   Tags

   HRV3C cleavage site and Maltose Binding Protein

   Expression

   Bacterial

   Mutation

   Promoter

   ptac

   Available since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAT-9251

   Plasmid

   #124226

   Purpose

   Bacterial SpCas9-HF1 expression

   Depositor

   Ervin Welker

   Article

   Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

   Insert

   SpCas9-HF1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   TetR/TetA

   Available since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUPD2 linker-RDF (GB2892)

   Plasmid

   #160604

   Purpose

   RDF sequence for C-terminal fusion with PhiC31 recombinase, together with a 18-nt linker sequence

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

   Insert

   RDF
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAV-U6+27-Linear-2_PolyU(1)_HairpinDistance(10)nts_Tornado-Corn

   Plasmid

   #159501

   Purpose

   Tests for the impacts 1 uracil in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination Module:Linear-2_PolyU(1)_HairpinDistance(10)nts
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6-27

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAV-U6+27-Linear-2_PolyU(4)_HairpinDistance(10)nts_Tornado-Corn

    Plasmid

    #159502

    Purpose

    Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.

    Depositor

    Julius Lucks

    Article

    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

    Insert

    Termination Module:Linear-2_PolyU(4)_HairpinDistance(10)nts
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6-27

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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