We narrowed to 21,788 results for: his

1. pBEL2265

   Plasmid

   #175962

   Purpose

   For expresson of LetA-[LetB PLL3->6] operon from E. coli. N-terminal his tag on LetA, araBAD promoter

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

   Inserts

   LetA
   LetB
   

   Use

   Tags

   8xHis 2xQH and TEV cleavage site on LetA

   Expression

   Bacterial

   Mutation

   Δ347-361, replace with 702-716

   Promoter

   araBAD

   Available since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. ppwd1.473.646.W601A

   Plasmid

   #137660

   Purpose

   Expresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

   Depositor

   Tara Davis, Melissa Jurica

   Article

   Adams et al Biochem J. 2015 Jul 15;469(2):223-33. do

   Insert

   ppwd1 (PPWD1 Human)
   

   Use

   Tags

   (His)6-thrombin

   Expression

   Bacterial

   Mutation

   473-646-W601A

   Promoter

   Available since

   Aug. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHBT-Mb(GDx-Clo_L10)

   Plasmid

   #183406

   Purpose

   Expresses Monobody GDx-Clo_L10 in E. coli

   Depositor

   Shohei Koide

   Article

   Kermani et al Nat Commun. 2020 Nov 27;11(1):6064. doi:

   Insert

   His tag-Avi tag-TEV cleavage site-Mb(GDx Clo_L10)
   

   Use

   Tags

   His tag-Avi tag-TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Sc NybbR eRF3∆165N (H348E)

   Plasmid

   #175420

   Purpose

   Encodes His-MBP-TEV-ybbR-H348E eRF3∆165N

   Depositor

   Joseph Puglisi

   Article

   Lawson et al Science. 2021 Aug 20;373(6557):876-882.

   Insert

   eRF3 (SUP35 Budding Yeast)
   

   Use

   Tags

   His-MBP-TEV and ybbR

   Expression

   Bacterial

   Mutation

   ∆165N, H348E

   Promoter

   T7

   Available since

   Oct. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJCC_084 Cas12i1 D647A

   Plasmid

   #171671

   Purpose

   For bacterial expression of Cas12i1 D647A (RuvC-inhibiting mutation) with an N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

   Insert

   Cas12i1 D647A
   

   Use

   Tags

   10X His, TEV protease cleavage site, and maltose-…

   Expression

   Bacterial

   Mutation

   changed aspartate 647 to alanine

   Promoter

   Available since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPD187 Tri-AND Gate, 2 repeats

   Plasmid

   #138940

   Purpose

   EYFP reporter vector for the Logic 37 AND 43 AND 158 2 repeats promoter

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   Logic 37 AND 43 AND 158 2 repeats
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Logic 37 AND 43 AND 158 2 repeats

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPD186 Tri-AND Gate, 1 repeat

   Plasmid

   #138939

   Purpose

   EYFP reporter vector for the Logic 37 AND 43 AND 158 1 repeat promoter

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   Logic 37 AND 43 AND 158 1 repeat
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Logic 37 AND 43 AND 158 1 repeat

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPD129 AND Gate, 4 repeats

   Plasmid

   #138938

   Purpose

   EYFP reporter vector for the Logic 37 AND 158 NOT 43 4 repeats promoter

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   Logic 37 AND 158 NOT 43 4 repeats
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Logic 37 AND 158 NOT 43 4 repeats

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPD127 AND Gate, 2 repeats

   Plasmid

   #138936

   Purpose

   EYFP reporter vector for the Logic 37 AND 158 NOT 43 2 repeats promoter

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   Logic 37 AND 158 NOT 43 2 repeats
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Logic 37 AND 158 NOT 43 2 repeats

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPD126 AND Gate, 1 repeat

    Plasmid

    #138935

    Purpose

    EYFP reporter vector for the Logic 37 AND 158 NOT 43 1 repeat promoter

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    Logic 37 AND 158 NOT 43 1 repeat
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Logic 37 AND 158 NOT 43 1 repeat

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPD270 ZF43x6-Compact(overlapping ZF37)_EYFP

    Plasmid

    #138934

    Purpose

    EYFP reporter vector for the ZF43x6-Compact(overlapping ZF37) promoter

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF43x6-Compact(overlapping ZF37)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    ZF43x6-Compact(overlapping ZF37)

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPD173 ZF43(AAAA)i

    Plasmid

    #138833

    Purpose

    Constitutive expression of ZF43(AAAA)i under the CMV promoter.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF43(AAAA)i
    

    Use

    Synthetic Biology

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    R6A, R15A, R43A, R71A

    Promoter

    CMV

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPD389 VPR-ZF43(AAAA)

    Plasmid

    #138826

    Purpose

    Constitutive expression of VPR-ZF43(AAAA) under the CMV promoter.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    VPR-ZF43(AAAA)
    

    Use

    Synthetic Biology

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    R6A, R15A, R43A, R71A

    Promoter

    CMV

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPD388 VP64-ZF43(AAAA)

    Plasmid

    #138822

    Purpose

    Constitutive expression of VP64-ZF43(AAAA) under the CMV promoter.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    VP64-ZF43(AAAA)
    

    Use

    Synthetic Biology

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    R6A, R15A, R43A, R71A

    Promoter

    CMV

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPD113 VP16-ZF43(AAAA)

    Plasmid

    #138818

    Purpose

    Constitutive expression of VP16-ZF43(AAAA) under the CMV promoter.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    VP16-ZF43(AAAA)
    

    Use

    Synthetic Biology

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    R6A, R15A, R43A, R71A

    Promoter

    CMV

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBEL1514

    Plasmid

    #155147

    Purpose

    Expresses MlaFEDCB operon with MlaF C-terminus deleted [1-246], His-tag on MlaD

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

    Insert

    mlaF(1-246)-mlaE-[6xHis-2xQH-TEV-mlaD]-mlaCB operon
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    araBAD

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBad-CA TAG186

    Plasmid

    #105837

    Purpose

    Expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 186 and C-terminal 6x his tag

    Depositor

    Ryan Mehl

    Article

    Mehl et al 2020

    Insert

    human carbonic anhydrase II thermostable variant (CA2 Human)
    

    Use

    Tags

    6x his tag

    Expression

    Bacterial

    Mutation

    TAG stop codon at site 186

    Promoter

    pBad/arabinose

    Available since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBad-CAts TAG97

    Plasmid

    #105668

    Purpose

    expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 97 and C-terminal 6x his tag

    Depositor

    Ryan Mehl

    Article

    Mehl et al 2020

    Insert

    human carbonic anhydrase II thermostable variant (CA2 Human)
    

    Use

    Tags

    6x his tag

    Expression

    Bacterial

    Mutation

    TAG stop codon at site 97

    Promoter

    pBad/arabinose

    Available since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CMYA5/pDEST17

    Plasmid

    #98115

    Purpose

    Express CMYA5 (aa 4039 – 4069) with a N-terminal 6x-His tag in bacteria

    Depositor

    Rita Shiang

    Article

    Hsiung et al J Psychiatr Res. 2019 Apr;111:8-15. doi:

    Insert

    CMYA5 (CMYA5 Human)
    

    Use

    Tags

    6X-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 21, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. Syn3D71

    Plasmid

    #14680

    Depositor

    Thomas Weimbs

    Article

    Sharma et al J Cell Biol. 2006 Jun 19. 173(6):937-48.

    Insert

    Syntaxin3 (STX3 Human)
    

    Use

    Tags

    2xMyc-His

    Expression

    Mammalian

    Mutation

    Insert is cloned into a modified vector; aa 1-71 …

    Promoter

    Available since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

21. Syn3D96

    Plasmid

    #14681

    Depositor

    Thomas Weimbs

    Article

    Sharma et al J Cell Biol. 2006 Jun 19. 173(6):937-48.

    Insert

    Syntaxin3 (STX3 Human)
    

    Use

    Tags

    2xMyc-His

    Expression

    Mammalian

    Mutation

    Insert is cloned into a modified vector; aa 1-96 …

    Promoter

    Available since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

22. Syn3D119

    Plasmid

    #14682

    Depositor

    Thomas Weimbs

    Article

    Sharma et al J Cell Biol. 2006 Jun 19. 173(6):937-48.

    Insert

    Syntaxin3 (STX3 Human)
    

    Use

    Tags

    2xMyc-His

    Expression

    Mammalian

    Mutation

    Insert is cloned into a modified vector; aa 1-119…

    Promoter

    Available since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

23. Syn3F35A

    Plasmid

    #13402

    Depositor

    Thomas Weimbs

    Article

    Sharma et al J Cell Biol. 2006 Jun 19. 173(6):937-48.

    Insert

    Syntaxin3 (STX3 Human)
    

    Use

    Tags

    Myc-His

    Expression

    Mammalian

    Mutation

    Insert is cloned into a modified vector. F35 of s…

    Promoter

    Available since

    Nov. 9, 2006

    Availability

    Academic Institutions and Nonprofits only

24. Syn3F36A

    Plasmid

    #13403

    Depositor

    Thomas Weimbs

    Article

    Sharma et al J Cell Biol. 2006 Jun 19. 173(6):937-48.

    Insert

    Syntaxin3 (STX3 Human)
    

    Use

    Tags

    Myc-His

    Expression

    Mammalian

    Mutation

    Insert is cloned into a modified vector. F36 of s…

    Promoter

    Available since

    Nov. 9, 2006

    Availability

    Academic Institutions and Nonprofits only

25. pLN432 (FuGW-G14p-mKate2)

    Plasmid

    #105187

    Purpose

    Module 3 - GAL4 synthetic promoter( with fourteen binding sites) drives mKate2

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    G14p-mKate2
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET28b(+)-HP0377

    Plasmid

    #39334

    Depositor

    SeWon Suh

    Insert

    oxidoreductase
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

27. pPMS-2008

    Plasmid

    #232288

    Purpose

    Protein Expression

    Depositor

    Jeremy Nathans

    Type

    Empty backbone

    Use

    Tags

    8X His-Avi-1D4

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Human SHIP2

    Plasmid

    #124645

    Purpose

    Expresses HIS tagged human SHIP2 in COS-7/HEK cells

    Depositor

    Christophe Erneux

    Article

    Pesesse et al J Biol Chem. 2001 Jul 27;276(30):28348-5

    Insert

    INPPL1 (INPPL1 Human)
    

    Use

    Tags

    His Xpress (N-terminal)

    Expression

    Mammalian

    Mutation

    coding sequence starts at amino acid 18 as calcul…

    Promoter

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. MtPT4

    Plasmid

    #117096

    Purpose

    protein expression in yeast

    Depositor

    Geoffrey Chang

    Insert

    MtPT4
    

    Use

    Tags

    His

    Expression

    Yeast

    Mutation

    Promoter

    AOX

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET29b-Cwc25

    Plasmid

    #111341

    Purpose

    his tagged Cwc25

    Depositor

    Christine Guthrie

    Insert

    CWC25
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge