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pTU-C-013

Plasmid

#124417

Purpose

Slot C, empty (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot C + terminator

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTU-A-010

Plasmid

#124418

Purpose

Slot A, contains mKO2-MarA (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot A with mKO2-MarA reporter + terminator + spacer

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTU-B-010

Plasmid

#124419

Purpose

Slot B, contains sfGFP-MarAn20 (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot B with sfGFP-MarAn20 reporter + terminator + spacer

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTU-C-010

Plasmid

#124420

Purpose

Slot C, contains RepA70-mKate2 (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot C with RepA70-mKate2 reporter + terminator

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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relA+_NS1

Plasmid

#100916

Purpose

expresses E.coli relA+ from the NS1 site in the S. elongatus PCC7942 genome

Depositor

Erin O'Shea

Article

Puszynska et al Cell Rep. 2017 Dec 12;21(11):3155-3165.

Insert

RelA fragment from E.coli

Use

Cyanobacteria cloning vector

Tags

FLAG

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pJet1.2 'rncS

Plasmid

#117129

Purpose

smc gene upstream homology region

Depositor

Stephan Gruber

Article

Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

Insert

'rncS

Use

Golden gate donor vector for gene targeting in ba…

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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puC19 preACT1(del6) AcAc

Plasmid

#111332

Purpose

T7 transcription

Depositor

Christine Guthrie

Article

Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

Insert

ACT1

Expression

Bacterial

Mutation

3' splice site AcAc

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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puC19 preACT1(WTdel6)

Plasmid

#111324

Purpose

for T7 transcription of wt actin pre-mRNA (del6)

Depositor

Christine Guthrie

Article

Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

Insert

ACT1

Expression

Bacterial

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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puC19 preACT1(AcAc)

Plasmid

#111327

Purpose

T7 transcription

Depositor

Christine Guthrie

Article

Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

Insert

ACT1

Expression

Bacterial

Mutation

3' splice site AcAc mutation in intron

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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puC19 preACT1(BrG)

Plasmid

#111326

Purpose

T7 transcription

Depositor

Christine Guthrie

Article

Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

Insert

ACT1

Expression

Bacterial

Mutation

branch site A to G mutation in intron

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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puC19 preACT1(BrC)

Plasmid

#111325

Purpose

T7 transcription

Depositor

Christine Guthrie

Article

Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

Insert

ACT1

Expression

Bacterial

Mutation

branch site A to C mutation in intron

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pTS36.6

Plasmid

#106264

Purpose

Expression of luc, ekivR, esyn1, mutated P2A

Depositor

Vera Meyer

Article

Schuetze et al Microb Cell Fact. 2017 Sep 25;16(1):162.

Insert

luc-P2A*-ekivR-P2A*-esyn1

Use

Luciferase and Synthetic Biology

Mutation

P2A: changed final proline to alanine

Promoter

Tet-On

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pJU225 Prp8-TAP

Plasmid

#111305

Purpose

Prp8-TAP

Depositor

Christine Guthrie

Article

Umen et al Genetics. 1996 Jun;143(2):723-39.

Insert

PRP8 (PRP8 Budding Yeast)

Expression

Yeast

Available Since

July 16, 2018

Availability

Academic Institutions and Nonprofits only

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pTS38.3

Plasmid

#106266

Purpose

Polycistronic expression of ekivR, esyn1, luc using P2A

Depositor

Vera Meyer

Article

Schuetze et al Microb Cell Fact. 2017 Sep 25;16(1):162.

Insert

ekivR-P2A-esyn1-P2A-luc

Use

Luciferase and Synthetic Biology

Promoter

Tet-On

Available Since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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pISH-Vmn1r168

Plasmid

#105995

Purpose

To prepare riboprobe for in situ hybridization

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Vmn1r168 (Vmn1r168 Mouse)

Use

To generate riboprobe

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pMST638_BB3_L_AF_hyg_KanOri_pyrG5

Plasmid

#90283

Purpose

BB3 recipient for homologous integration in pyrG locus with linker AF

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Expression

Bacterial

Available Since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

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FoRC.PGm

Plasmid

#84586

Purpose

Drosophila P-element transgenesis vector for cloning rescue transgenes. Cloning site is flanked by FRT sequences and contains constitutive GFP reporter.

Depositor

Francesca Pignoni

Article

Zhou et al Genesis. 2016 Oct 3. doi: 10.1002/dvg.22

Type

Empty backbone

Expression

Insect

Available Since

Dec. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pEZ04

Plasmid

#81127

Purpose

pACSA_J23109_tetR PEZ1_gfp

Depositor

Brian Pfleger

Article

Zess et al Biotechnol Bioeng. 2016 Feb;113(2):424-3

Insert

GFP

Promoter

PEZ1

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pEZ12

Plasmid

#81138

Purpose

pACSA_J23113_tetR PEZ4_gfp

Depositor

Brian Pfleger

Article

Zess et al Biotechnol Bioeng. 2016 Feb;113(2):424-3

Insert

GFP

Promoter

PEZ4

Available Since

Oct. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-myc

Plasmid

#67172

Purpose

CRISPaint universal donor for myc fusion tagging

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

myc

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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